Genetic Diversity of Local Greek and Bulgarian Grapevine (Vitis Vinifera L.) Varieties

Papapetrou, Maria; Loukovitis, Dimitrios; Papadopoulos, O.; Kazlari, Zoi; Peristeraki, Anastasia; Arsenova, Slavina; Bardarova, Desislava; Doncheva, Desislava; Theocharis, Serafeim; Karagiannidis, Constantinos; Koundouras, Stéfanos; Γιαννακούλα, Αναστασία; Aggelopoulos, Stamatis; Chatziplis, Dimitrios

doi:10.3390/d12070273

Cited by 5 publications

(2 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The standard set of 9 microsatellite loci has been developed for DNA fingerprinting of grape ge notypes (Bowers et al, 1999;This et al, 2004;VIVC, 2021). The use of DNA markers in order to identify grape varieties and study their polymorphism made it possible to clarify many questions regarding homonym and synonym varieties, as well as to determine the most genetically similar and dis tant genotypes (Crespan, Milani, 2001;Fossati et al, 2001;Vokurka et al, 2003;Santiago et al, 2005;MorenoSanz et al, 2008;Cipriani et al, 2010;Goryslavets et al, 2015;Raimondi et al, 2015;Mandić et al, 2019;Papapetrou et al, 2020;Pastore et al, 2020). For example, using a genetic analysis of 35 autochthonous varieties of Bosnia and Herze govina at 9 standard microsatellite loci, several synonyms and homonyms were identified.…”

Section: Introductionmentioning

confidence: 99%

Genetic polymorphism of local Abkhazian grape cultivars

et al. 2022

View full text Add to dashboard Cite

Local grape cultivars from different countries of the world are an important part of the gene pool of this culture. Of particular interest are the genotypes of the most ancient regions of viticulture. The territories of the subtropical zone of Georgia and the central part of Abkhazia belong to one of the centers of origin of the cultural grapevine. The purpose of the work was to genotype native Abkhazian grape cultivars, to study their genetic diversity based on DNA profiling data and to compare them with the genotypes of local varieties of other viticultural regions. Samples of plants were taken on the territory of the Republic of Abkhazia in private farmsteads and in the collection of the agricultural firm “Vina i Vody Abkhazii“ (“Wines and Waters of Abkhazia”). The genotyping of the Abkhazian cultivars Avasirhva, Agbizh, Azhapsh, Azhizhkvakva, Azhikvaca, Atvizh, Atyrkuazh, Achkykazh, Kachich was carried out using 14 DNA markers, 9 of which are standard microsatellite markers recommended for the identification of grape varieties. To improve our knowledge about the sizes of the identified alleles, we used the DNA of grape cultivars with a known allelic composition at the analyzed loci. Statistical analysis of the data showed that the observed heterozygosity for the analyzed loci exceeded expected values, which indicates a genetic polymorphism of the studied sample of varieties. Evaluation of genetic similarity within the analyzed group based on the results of genotyping at 14 loci showed that the cultivars Kachich and Azhapsh differed from the other Abkhazian varieties. The obtained DNA profiles of the Abkhazian cultivars were checked for compliance with DNA-fingerprints of grape varieties in the Vitis International Variety Catalogue. The Georgian varieties Azhizhkvakva and Tsitska turned out to be synonyms according to DNA profiles, two varieties from the Database (Italian Albana bianca and Georgian Ojaleshi) have differences in DNA-fingerprints from the varieties Atyrkuazh and Azhikvatsa only in one allele, respectively. When comparing the identified Abkhazian grape genotypes, their difference from the sample of Dagestan, Don, Greek, Turkish, Italian, Spanish, and French varieties and genetic similarity with the genotypes of Georgian grapes were shown.

show abstract

Section: Introductionmentioning

confidence: 99%

Genetic polymorphism of local Abkhazian grape cultivars

et al. 2022

View full text Add to dashboard Cite

show abstract

“…The study of the gene pool of grapevines in Abkhazia is important both for the purpose of expanding knowledge about native forms of grapes in various regions, and for searching for sources of valuable traits. Currently, the study of the gene pool is carried out using methods of molecular genetic analysis [2][3][4][5][6][7][8][9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Study of the unknown vine genotype found in Abkhazia

et al. 2020

View full text Add to dashboard Cite

The territory of Abkhazia belongs to the region of origin of the cultivated grapevine. Wild forms, intermediate forms between wild and cultivated grapevines and a variety of local native cultivars grow here. In the one of the private yards, a grapevine was found, the name and exact origin of which is not known. The vine showed no signs of fungal diseases in the humid conditions of the region during the absence of chemical treatment. The grape harvest was used to make red wine. DNA-fingerprinting of the unknown genotype was performed to determine its origin. Analysis of the identified DNA profile for 9 SSR loci in the international VIVC database did not reveal coincides with any known cultivar. The methods of Principal coordinates analysis and Bayesian analysis were used to compare the unknown genotype with the genotypes of Vitis vinifera L of several geographic groups and varieties - interspecific hybrids. The closest genotype to the unknown form is the variety Isabella (V. labrusca L. × V. vinifera L).

show abstract

Diversity and association mapping assessment of an untouched native grapevine genetic resource by iPBS retrotransposon markers

Güler

Karadeniz

Özer

et al. 2023

Genet Resour Crop Evol

View full text Add to dashboard Cite

Grape is one of the most produced horticultural crops, being one of the oldest domesticated plants that undergo genetic differentiation due to the natural crossing, bud mutations, and the effect of the continuously changing demand for wine types and table grapes. Recognition and discrimination of autochthonous varieties are the rst steps for breeding studies. In this study, an autochthonous grapevine population was recognized by iPBS (Inter primer binding sites) retrotransposon markers and the genetic relationships with some other varieties from Turkey and Europe. The trait-associated loci were determined by GLM (general linear model) and MLM (mixed linear model) analysis. Eight iPBS markers generated 136 loci, of which 106 were polymorphic. The neighbor-joining method clustered the genotypes and standard varieties into three main and seven subclusters. Structure analysis also grouped the grapes into seven populations. Molecular variance analysis showed that the variability occurred mainly among individuals. In the association mapping, 36 loci were correlated with quantitative traits in GLM, whereas 21 loci were correlated in MLM. A remarkable diversity among the autochthonous grape population, even among the same-named individuals, was determined by diversity assessments. Distinct genotypes with desired characteristics will be valuable in breeding studies. The loci determined by both mapping approaches might help to create functional markers to select genotypes with the desired traits.

show abstract

Genetic Diversity of Local Greek and Bulgarian Grapevine (Vitis Vinifera L.) Varieties

Cited by 5 publications

References 22 publications

Genetic polymorphism of local Abkhazian grape cultivars

Genetic polymorphism of local Abkhazian grape cultivars

Study of the unknown vine genotype found in Abkhazia

Diversity and association mapping assessment of an untouched native grapevine genetic resource by iPBS retrotransposon markers

Contact Info

Product

Resources

About