Genetic diversity of <i>Plasmodium falciparum</i> field isolates from south western Nigeria

Abstract

Background: Plasmodium falciparum the main causative agent of malaria is an important public health vector. With the use of PCR, its genetic diversity has been extensively studied with dearth information from Nigeria.

Genetic Diversity and Complexity of Plasmodium Falciparum Infections in Lagos, Nigeria

“…However, the parasite populations in low transmission areas have limited genetic diversity and most infections are monoclonal [26,27], hence compromising the suitability of antigenic markers as genotyping tools for drug efficacy tracking. In agreement with Oyedeji et al [23], we observed a limited number of parasite genotypes and low multiplicity of P. falciparum infections compared with observations from neighbouring towns [22]. This heterogeneity in P. falciparum genetic characteristics also has an important implication on the identification of precise target molecules for rational vaccine design.…”

“…Contrary to earlier reports from neighbouring states, Oyo [21] and Ogun [22], our investigation has revealed a predominant distribution of msp 1 locus alleles belonging to the K1 family in the Lagos population of P. falciparum. Also, in contrast with reports from the north-central region of the country [23], the proportion of the parasite isolates possessing msp 2 alleles belonging to FC27 family was higher than those with 3D7, pointing to spatial dynamics in the genetic profile of P. falciparum populations in the country.…”

Efficiency of histidine rich protein II-based rapid diagnostic tests for monitoring malaria transmission intensities in an endemic area

“…Hence, HRP-2 RDTs could serve as a tool for mapping intensities of transmission in untreated population, because HRP-2 protein can be detected both in asexual parasites and from young gametocytes of P. falciparum. In P. falciparum infected red blood cells, merozoite surface protein 2 (msp 2) is a highly expressed; a study has reported the diversity of falciparum infections in this area, thus can reliably be used to confirm P. falciparum infections [17]. RDTs can solve problems of misdiagnosis at low parasitemia, and missed detection by microscopy which can be detected through parasite antigens [7,9,18].…”

Allelic diversity of merozoite surface protein-1&nbsp;(MSP 1) and MSP 2 genes, and clinical manifestations of Plasmodium falciparum malaria cases in Aceh, Indonesia

“…for the MSP1 and MSP2 respectively. This nding is slightly higher than the MOI data from Myanmar [17] and Southwest Paci c [18] but lower than Thailand, Kenya and Burkina Faso [19][20][21][22]. The difference in MOI can be attributed to several factors such as differences in geographical areas, intensity of malaria transmission, and difference in age of study population and mean parasite density in the study population [23][24][25].…”