Genetic diversity of Macrophomina phaseolina isolates from certain agro-climatic regions of India by using RAPD markers

Babu, Bandamaravuri Kishore; Reddy, S. S.; Yadav, Mukesh; Sukumar, M.; Mishra, Vijendra; Saxena, Anil Kumar; Arora, Dilip K.

doi:10.1007/s12088-010-0033-x

Cited by 24 publications

(15 citation statements)

References 13 publications

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“…In agreement with our result, a lot of molecular studies showed a high level of polymorphism in this fungus when isolates from different hosts or geographical origins were compared using different molecular tools Archives of Phytopathology and Plant Protection 971 (Jones et al 1998;Vandemark et al 2000;Mayek-Perez et al 2001;Su et al 2001;Almeida et al 2003). Other recent studies have demonstrated the genetic diversity among M. phaseolina isolates (Jana et al 2005a(Jana et al , 2005bBeas-Fernandez et al 2006;Das et al 2006;Reyes-Franco et al 2006;Aboshosha et al 2007;Omar et al 2007;Rajkumar and Kuruvinashetti 2007;Purkayastha et al 2008;Aghakhani and Dubey 2009;Babu et al 2010;Baird et al 2010;Saleh et al 2010). The high genetic diversity of this pleomorphic fungal species is reflected not only in the isolates from distinct hosts and geographical origins but also within the isolates collected from a single host or geographical origin.…”

Section: Discussionsupporting

confidence: 89%

“…Molecular markers are the powerful tools for assessing genetic variation and elucidating genetic relationships within and among species (Chakravarthi and Naravaneni 2006). Molecular methods used for differentiating M. phaseolina populations have included restriction fragment length polymorphism of ribosomal DNA-Internal Transcribed Spacer (rDNA-ITS) regions (Su et al 2001;Almeida et al 2003;Purkayastha et al 2006;Aghakhani and Dubey 2009), random amplified polymorphic DNA (Pecina-Quintero et al 2001;Su et al 2001;Almeida et al 2003Almeida et al , 2008Jana et al 2003;Das et al 2006;Meena et al 2006;Purkayastha et al 2006;Aboshosha et al 2007;Omar et al 2007; Rajkumar and Kuruvinashetti 2007;Aghakhani and Dubey 2009;Zade et al 2009;Babu et al 2010), amplified fragment length polymorphism (AFLP; Vandemark et al 2000;Mayek-Perez et al 2001;Pecina-Quintero et al 2001;Reyes-Franco et al 2006;Brooker et al 2008;Saleh et al 2010), universal rice primer polymerase chain reaction (PCR; Jana et al 2005b), inter-simple sequence repeats (ISSRs; Jana et al 2005a;Purkayastha et al 2008), repetitive sequence-based PCR (Purkayastha et al 2008) and simple sequence repeat (SSR; Baird et al 2010). ISSR technique is a PCR-based method, which uses primers designed based on the microsatellite sequences, usually 16-to 25-bp long, and amplifies the flanking regions of microsatellites which appear as multiple genomic loci (Reddy et al 2002).…”

Section: Introductionmentioning

confidence: 99%

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Intraspecies diversity ofMacrophomina phaseolinain Iran

Mahdizadeh

Safaie

Goltapeh

et al. 2012

Archives Of Phytopathology And Plant Protection

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To study the pathogenic and genetic diversity of the Macrophomina phaseolina in Iran, 52 isolates of the fungus were isolated from 24 host plants across the 14 Iranian provinces. All isolates were confirmed to the species based on the species-specific primers. The aggressiveness of M. phaseolina isolates was evaluated on the common bean. Based on the pathogenicity tests, M. phaseolina isolates from the different hosts displayed different levels of aggressiveness on the common beans. The results showed that there was significant variation in the aggressiveness of the pathogen; however, there was no distinct pattern of differentiation based on the host or geographical origin linked to the virulence of the isolates, as frequently the isolates from the same host or geographical origin had different levels of aggressiveness. Inter-simple sequence repeat (ISSR) markers were used to assess the genetic diversity of the fungus. The unweighted pair-group method, using arithmetic mean clustering of data, showed that isolates did not clearly differentiate to the specific group according to the host or geographical origins; however, usually the isolates from the same host or the same geographical origin tend to group nearly. Our results did not show a correlation between the genetic diversity based on the ISSR and pathogenic patterns on common bean in the greenhouse. Similar to the M. phaseolina populations in the other countries, the Iranian isolates were highly diverse based on the pathogenic and genotypic characteristics.

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Section: Discussionsupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Intraspecies diversity ofMacrophomina phaseolinain Iran

Mahdizadeh

Safaie

Goltapeh

et al. 2012

Archives Of Phytopathology And Plant Protection

View full text Add to dashboard Cite

show abstract

“…A number of molecular studies elsewhere have shown a high level of polymorphism in this fungus when isolates from different host or geographical origins were compared using different molecular tools (Almeida et al, 2003;Jones et al, 1998;Mayek-Perez et al, 2001;Su et al, 2001;Vandemark et al, 2000). Other recent studies have demonstrated the genetic diversity among M. phaseolina isolates (Aboshosha et al, 2007;Aghakhani and Dubey, 2009;Babu et al, 2010;Baird et al, 2010;Beas-Fernandez et al, 2006;Das et al, 2006;Jana et al, 2005a;Jana et al, 2005b;Omar et al, 2007;Purkayastha et al, 2008;Rajkumar and Kuruvinashetti, 2007;Reyes-Franco et al, 2006;Saleh et al, 2010). The high genetic diversity of this pleomorphic fungal species is reflected not only in isolates from distinct hosts and geographical origins but also within isolates collected from a single host or geographical origin.…”

Section: Resultsmentioning

confidence: 99%

“…Different molecular methods have been used for differentiating M. phaseolina populations including Restriction Fragment Length Polymorphism (RFLP) of rDNA-ITS regions (Aghakhani and Dubey, 2009;Almeida et al, 2003;Purkayastha et al, 2006;Su et al, 2001), Random Amplified Polymorphic DNA (RAPD) (Aboshosha et al, 2007;Aghakhani and Dubey, 2009;Almeida et al, 2003;Almeida et al, 2008;Babu et al, 2010;Das et al, 2006;Jana et al, 2003;Omar et al, 2007;Purkayastha et al, Rajkumar and Kuruvinashetti, 2007;Su et al, 2001;Zade et al, 2009), Amplified Fragment Length Polymorphism (AFLP) (Brooker et al, 2008;Mayek-Perez et al, 2001;Reyes-Franco et al, 2006;Saleh et al, 2010;Vandemark et al, 2000), Universal Rice Primer PCR (URP-PCR) (Jana et al, 2005b), Inter simple sequence repeats (ISSR) (Jana et al, 2005a;Purkayastha et al, 2008), Repetitive SequenceBased Polymerase Chain Reaction (Rep-PCR) (Purkayastha et al, 2008) and SSR (Baird et al, 2010). Inter simple sequence repeat (ISSR) markers are powerful tools which can be utilized to access the variation in the flanking regions of microsatellite loci that are dispersed throughout all genomes (Zietkiewicz et al, 1994).…”

mentioning

confidence: 99%

Diversity of Macrophomina phaseolina Based on Morphological and Genotypic Characteristics in Iran

Mahdizadeh¹,

Safaie²,

Goltapeh³

2011

The Plant Pathology Journal

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Fifty two Macrophomina phaseolina isolates were recovered from 24 host plant species through the 14 Iranian provinces. All isolates were confirmed to species using species-specific primers. The colony characteristics of each isolate were recorded, including chlorate phenotype, relative growth rate at 30°C and 37°C, average size of microsclerotia, and time to microsclerotia formation. The feathery colony phenotype was the most common (63.7%) on the chlorate selective medium and represented the chlorate sensitive phenotype of the Iranian Macrophomina phaseolina population. Meantime, inter simple sequence repeats (ISSR) Markers were used to assess the genetic diversity of the fungus. Unweighted pair-group method using arithmetic means (UPGMA) clustering of data showed that isolates did not clearly differentiate to the specific group according to the host or geographical origins, however, usually the isolates from the same host or the same geographic origin tend to group nearly. Our results did not show a correlation between the genetic diversity based on the ISSR and phenotypic characteristics. Similar to the M. phaseolina populations in the other countries, the Iranian isolates were highly diverse based on the phenotypic and the genotypic characteristics investigated and needs more studies using neutral molecular tools to get a deeper insight into this complex species.

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“…However, variability among populations of M. phaseolina may result in such resistance being short‐lived (Hernández‐Delgado et al ., ; Gupta et al ., ; Iqbal & Mukhtar, ). The high genetic diversity exhibited by M. phaseolina is reflected not only in the numbers of genetically unique isolates obtained from a single host plant or a geographical location, but also from distinct hosts and geographical origins (Babu et al ., ; Sarr et al ., ). In addition, isolates from a single host species may vary in pathogenicity on different cultivars of that species, suggesting the existence of distinct physiological races of M. phaseolina (Sulaiman & Patil, ; Mahdizadeh et al ., ).…”

Section: Introductionmentioning

confidence: 96%

Heterokaryosis and diploid formation among Brazilian isolates of Macrophomina phaseolina

et al. 2018

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Heterokaryosis is the association of genetically distinct nuclei in a common hyphal cytoplasm, and is a process involved in the generation of fungal variation. The fusion of the distinct nuclei within a heterokaryotic hypha produces a heterozygous diploid nucleus and is one of the stages of the parasexual cycle. A heterokaryon's viability depends on the isolates’ genetic constitution with regard to loci called het (heterokaryon incompatibility) or vic (vegetative incompatibility). The current study evaluates for the first time the diversity of vegetative compatibility reactions in isolates of Macrophomina phaseolina from different hosts. Complementary nit (nitrate non‐utilizing) mutants of each isolate were obtained and paired in all possible combinations. Isolates were classified in vegetative compatibility groups (VCG) according to their ability to form viable heterokaryons. Ten VCGs were identified, two of them containing two isolates, and the remainder containing a single isolate. When grown on basal medium, heterokaryons produced both (i) auxotrophic segregants exhibiting the same phenotype as the paired mutants; and (ii) a fast‐growing sector, characterized as a heterozygous diploid sector, named D653, which showed a nit+ phenotype with a growth rate similar to the original wild isolate. When grown in the presence of benomyl, a haploidizing agent, D653 produced auxotrophic haploid segregants exhibiting the nit phenotypes of the crossed mutants. The results demonstrate for the first time the ability of M. phaseolina isolates to form viable heterokaryons and heterozygous diploid nuclei, suggesting that the parasexual cycle may be an alternative source of genetic variability in this species.

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Genetic diversity of Macrophomina phaseolina isolates from certain agro-climatic regions of India by using RAPD markers

Cited by 24 publications

References 13 publications

Intraspecies diversity ofMacrophomina phaseolinain Iran

Intraspecies diversity ofMacrophomina phaseolinain Iran

Diversity of Macrophomina phaseolina Based on Morphological and Genotypic Characteristics in Iran

Heterokaryosis and diploid formation among Brazilian isolates of Macrophomina phaseolina

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