Genetic Diversity of Magra Sheep from India Using Microsatellite Analysis

Arora, Reena; Bhatia, Sabhyata

doi:10.5713/ajas.2006.938

Cited by 36 publications

(42 citation statements)

References 16 publications

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“…However, a higher number of alleles for each locus showed that all the markers used were appropriate to analyze diversity in studied breeds. The level of variation depicted by number of alleles at each locus was similar to earlier reports on sheep breeds (Arora and Bhatia, 2006;Jakaria et al, 2012;Hepsibha et al, 2013;Crispim et al, 2014). Among the types, the mean of PIC ranged from 0.72 in LRB-PAK to 0.88 in ARB, and Shannon information index ranged from 1.58 in KER-PAK to 2.27 in ARB.…”

Section: Discussionsupporting

confidence: 72%

Using microsatellite markers to analyze genetic diversity in 14 sheep types in Iran

2017

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Abstract. Investigation of genetic relationship among populations has been traditionally based on the analysis of allele frequencies at different loci. The prime objective of this research was to measure the genetic polymorphism of five microsatellite markers (McMA2, BM6444, McMA26, HSC, and OarHH35) and study genetic diversity of 14 sheep types in Iran. Genomic DNA was extracted from blood samples of 565 individuals using an optimized salting-out DNA extraction procedure. The polymerase chain reaction (PCR) was successfully performed with the specific primers. Some locus-population combinations were not at Hardy-Weinberg equilibrium (P < 0.05). The microsatellite analysis revealed high allelic and gene diversity in all 14 breeds. Pakistani and Arabi breeds showed the highest mean number of alleles (11.8 and 11 respectively), while the highest value for polymorphic information content was observed for the Arabi breed (0.88). A UPGMA (unweighted pair group method with arithmetic mean) dendrogram based on the Nei's standard genetic distance among studied breeds showed a separate cluster for Arabi and Pakistani breeds and another cluster for other breeds. The Shannon index (H0) for McMA2, BM6444, McMA26, HSC, and OarHH35 was 2.31, 2.17, 2.27, 2.04 and 2.18, respectively, and polymorphic information content (PIC) values were 0.88, 0.92, 0.87, 0.84, and 0.86 for McMA2, BM6444, McMA26, HSC, and OarHH35, respectively. The high degree of variability demonstrated within the studied sheep types implies that these populations are rich reservoirs of genetic diversity that must be preserved.

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Section: Discussionsupporting

confidence: 72%

Using microsatellite markers to analyze genetic diversity in 14 sheep types in Iran

2017

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“…In the present study all markers were highly informative (PIC>0.5; Arora et al, 2006). These observations suggested the additional utility of these markers for population assignment (Arora et al, 2010) in indigenous sheep and gene mapping (Kayang et al, 2002).…”

Section: Discussionmentioning

confidence: 57%

Genetic characterization of Colombian indigenous sheep

Ocampo

Martínez

Rocha

et al. 2017

Rev Colomb Cienc Pecu

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Background: indigenous breeds are important for poor farmers because of their natural selection against harsh environments and adaptation to regional conditions. However, inbreeding of indigenous sheep populations has increased in Colombia due to indiscriminate cross-breeding with foreign animals and lack of reproductive controls, with subsequent loss in productivity, which poses a great risk for the conservation of valuable genes. Objective: to determine the genetic diversity in Colombian indigenous sheep by using a panel of 10 microsatellite molecular markers. Methods: blood samples from 362 individuals from 43 farms in 11 Colombian provinces were genotyped and analyzed for a panel of 10 microsatellite markers. Results: a total of 134 alleles were found (13.4 alleles/locus on average) with a range of observed and expected heterozygosity of 0.428 to 0.831 and 0.615 to 0.855, respectively, and 0.742 polymorphic information content (PIC). The average Wright F-statistics (F IS ) of the breeds was 0.107, suggesting moderate levels of inbreeding. Colombian sheep showed a low level of genetic differentiation among breeds (F ST = 0.054) and STRUCTURE analysis showed complex patterns of admixture in the breeds. Conclusion: overall, Colombian sheep have high genetic variability, which is very important for future conservation programs and genetic improvement.

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“…Microsatellites have been widely used to characterize sheep breeds of several countries (Baumung et al 2006;Peter et al 2007;Cinkulov et al 2008;Legaz et al 2008;Dalvit et al 2008;Alvarez et al 2009). Microsatellite analysis of the Nali, Chokla , Marwari (Singh et al 2007) and Magra (Arora and Bhatia 2006) breeds has been reported previously based on genotyping by silver staining methods, but a comparative study of all six breeds has not yet been described. Their unique characteristics and dwindling numbers make an analysis of their genetic diversity and relationships urgent.…”

Section: Introductionmentioning

confidence: 95%

Genetic Variation and Relationship of Six Indian Sheep Breeds Adapted to the Northwestern Arid Zone of Rajasthan

2011

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This study illustrates the genetic diversity and relationships within and among six Indian sheep breeds of the northwestern arid region of Rajasthan, based on microsatellite markers. The range of allele diversity was 7.72-9.56, and gene diversity was 0.686-0.766, revealing that these breeds possessed substantial amounts of genetic diversity. Positive F (IS) values suggested a deficit of heterozygotes in all six breeds. Despite the declining status of the Marwari, Chokla, Jaisalmeri, Magra and Pugal breeds, an absence of a recent genetic bottleneck was evident from the data. The genetic differentiation estimate (F (ST) = 6.1%) suggested low levels of differentiation between the breeds. Genetic distance estimates revealed a close relationship between the Magra-Pugal and Nali-Jaisalmeri breed pairs. This information forms a framework for designing genetic management and conservation programs for these valuable ovine breeds.

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Genetic Diversity of Magra Sheep from India Using Microsatellite Analysis

Cited by 36 publications

References 16 publications

Using microsatellite markers to analyze genetic diversity in 14 sheep types in Iran

Using microsatellite markers to analyze genetic diversity in 14 sheep types in Iran

Genetic characterization of Colombian indigenous sheep

Genetic Variation and Relationship of Six Indian Sheep Breeds Adapted to the Northwestern Arid Zone of Rajasthan

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