Genetic diversity of Microcystis cyanophages in two different freshwater environments

Nakamura, Ginji; Kimura, Shigeko; Sako, Yoshihiko; Yoshida, Takashi

doi:10.1007/s00203-014-0980-4

Cited by 12 publications

(10 citation statements)

References 39 publications

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“…Following the identification of NblA as a pivotal component of PBS degradation in S. elongatus , involvement of NblA in the decomposition of PBSs in a red alga (Kawakami et al, 2009) and in diverse cyanobacteria was reported (reviewed in Schwarz and Forchhammer, 2005). Interestingly, genes encoding NblA homologues were also identified in cyanophages, supporting the hypothesis that PBS degradation driven by phage-encoded NblA provides nutrients and is thus advantageous for the phage lifecycle (Yoshida et al, 2008;Gao et al, 2012;Yoshida-Takashima et al, 2012;Nakamura et al, 2014;Ou et al, 2015;Voorhies et al, 2016).…”

Section: Pbs Disassembly and Degradationmentioning

confidence: 86%

Nitrogen chlorosis in unicellular cyanobacteria – a developmental program for surviving nitrogen deprivation

Forchhammer

Schwarz

2018

Environmental Microbiology

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Summary Cyanobacteria evolved sophisticated mechanisms allowing them to cope with environmental depletion of combined nitrogen. Here, we describe progress in understanding the processes involved in acclimation of nondiazotrophic cyanobacteria to nitrogen shortage, known as nitrogen chlorosis. The process includes immediate metabolic changes and degradation of light harvesting complexes as well as long‐term acclimation responses. Consequently, quiescent cells substantially differing from vegetative cells are obtained. Thus, the process leading to these considerable metabolic and morphological changes is referred to as a developmental program. Current understanding of the relevant regulatory processes depicts an intricate mechanism involving modulation of transcription activators by proteinaceous interacting components, as well as by small metabolites reporting the energy status and carbon–nitrogen balance of the cell. In addition, we describe in detail the quiescent state characterizing cells under prolonged starvation and the process of recovery from this dormant chlorotic state. Accumulated data provide an in depth understanding of the mechanisms accompanying the cycling of cyanobacterial cells between vegetative growth, the quiescent‐state and the recovery program, allowing them to regain proliferative growth upon nutrient replenishment.

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Section: Pbs Disassembly and Degradationmentioning

confidence: 86%

Nitrogen chlorosis in unicellular cyanobacteria – a developmental program for surviving nitrogen deprivation

Forchhammer

Schwarz

2018

Environmental Microbiology

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“…Additionally, the interaction of NblA with phycobilisomes attached to thylakoid membranes supports a role for NblA in the sequential disassembly of this large pigment complex (Sendersky et al ., , ). Genes encoding homologues of NblA were identified in several cyanophages – a finding suggesting that stimulation of phycobilisome degradation during infection is beneficial for the phage lifecycle (Yoshida et al ., ; Gao et al ., ; Yoshida‐Takashima et al ., ; Nakamura et al ., ; Ou et al ., ; Voorhies et al ., ).…”

Section: Introductionmentioning

confidence: 98%

Decomposition of cyanobacterial light harvesting complexes: NblA‐dependent role of the bilin lyase homolog NblB

2018

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Phycobilisomes, the macromolecular light harvesting complexes of cyanobacteria are degraded under nutrient-limiting conditions. This crucial response is required to adjust light excitation to the metabolic status and avoid damage by excess excitation. Phycobilisomes are comprised of phycobiliproteins, apo-proteins that covalently bind bilin chromophores. In the cyanobacterium Synechococcus elongatus, the phycobiliproteins allophycocyanin and phycocyanin comprise the core and the rods of the phycobilisome, respectively. Previously, NblB was identified as an essential component required for phycocyanin degradation under nutrient starvation. This protein is homologous to bilin-lyases, enzymes that catalyze the covalent attachment of bilins to apo-proteins. However, the nblB-inactivated strain is not impaired in phycobiliprotein synthesis, but rather is characterized by aberrant phycocyanin degradation. Here, using a phycocyanin-deficient strain, we demonstrate that NblB is required for degradation of the core pigment, allophycocyanin. Furthermore, we show that the protein NblB is expressed under nutrient sufficient conditions, but during nitrogen starvation its level decreases about two-fold. This finding is in contrast to an additional component essential for degradation, NblA, the expression of which is highly induced under starvation. We further identified NblB residues required for phycocyanin degradation in vivo. Finally, we demonstrate phycocyanin degradation in a cell-free system, thereby providing support for the suggestion that NblB directly mediates pigment degradation by chromophore detachment. The dependence of NblB function on NblA revealed using this system, together with the results indicating presence of NblB under nutrient sufficient conditions, suggests a rapid mechanism for induction of pigment degradation, which requires only the expression of NblA.

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“…Molecular genomic analysis of this strain led to the identification of the g91 gene, which was characterized as a viral sheath protein-encoding gene [12,13,19,26,33,34].…”

Section: Introductionmentioning

confidence: 99%

Cyanophages Infection of Microcystis Bloom in Lowland Dam Reservoir of Sulejów, Poland

et al. 2015

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An increased incidence of cyanobacterial blooms, which are largely composed of toxigenic cyanobacteria from the Microcystis genus, leads to a disruption of aquatic ecosystems worldwide. Therefore, a better understanding of the impact of environmental parameters on the development and collapse of blooms is important. The objectives of the present study were as follows: (1) to investigate the presence and identity of Microcystis-specific cyanophages capable of cyanobacterial cell lysis in a lowland dam reservoir in Central Europe; (2) to investigate Microcystis sensitivity to phage infections with regard to toxic genotypes; and (3) to identify key abiotic parameters influencing phage infections during the summer seasons between 2009 and 2013. Sequencing analysis of selected g91 gene amplification products confirmed that the identified cyanophages belonged to the family Myoviridae (95 % homology). Cyanophages and Microcystis hosts, including toxic genotypes, were positively correlated in 4 of the 5 years analyzed (r = 0.67-0.82). The average percentage of infected Microcystis cells varied between 0.1 and 32 %, and no particular sensitivity of the phages to toxigenic genotypes was recorded. The highest number of cyanophages (>10(4) gene copy number per microliter) was observed in the period preceded by the following: an increase of the water retention time, growth of the water temperature, optimum nutrient concentrations, and the predomination of Microcystis bloom.

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Genetic diversity of Microcystis cyanophages in two different freshwater environments

Cited by 12 publications

References 39 publications

Nitrogen chlorosis in unicellular cyanobacteria – a developmental program for surviving nitrogen deprivation

Nitrogen chlorosis in unicellular cyanobacteria – a developmental program for surviving nitrogen deprivation

Decomposition of cyanobacterial light harvesting complexes: NblA‐dependent role of the bilin lyase homolog NblB

Cyanophages Infection of Microcystis Bloom in Lowland Dam Reservoir of Sulejów, Poland

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