Genetic diversity of norovirus in outbreaks of gastroenteritis in Seoul

Kim, Eun-Jeung; Cheon, Doo‐Sung; Jeong, Hae-Sook; Park, Sanghun; Kim, Chang-Kyu; Choi, Seongseon; Oh, Seah; Cho, Seokju; Han, Ki-Young; Kim, Minyoung

doi:10.1007/s10068-010-0153-4

Cited by 3 publications

(1 citation statement)

References 18 publications

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“…RNA from NoV GII-positive specimens was analyzed by RT-PCR using a semi-nested GII-specific primer set (GIIF1M/GIIR1M for a first-round PCR and GIIF3M/GIIR1M for a second-round PCR) to amplify the capsid gene ( Table 1 ). RT-PCR was performed using a One-Step RT-PCR Kit (Qiagen) under previously described amplification conditions [ 19 , 20 ]. PCR products were purified using a QIAquick PCR Purification Kit (Qiagen).…”

Section: Methodsmentioning

confidence: 99%

Emergence of Norovirus GII.17 Variants among Children with Acute Gastroenteritis in South Korea

et al. 2016

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Of 1,050 fecal specimens collected from January 2013 to August 2015 from children with acute gastroenteritis, 149 (14.2%) were found to be positive for norovirus. Norovirus GII was the most predominant genogroup (98.65%; 147 of 149). The genotypes detected in this study were GI (2; 1.3%), GII.Pe-GII.4 (109; 73.1%), GII.P17-GII.17 (16; 10.7%), GII.P12-GII.3 (8; 5.4%), GII.P12-GII.12 (8; 5.4%), GII.P4-GII.4 (5; 3.4%), and the recombinant GII.Pe-GII.17 (1; 0.7%). Of these, the novel GII.17 strain was the second most predominant, and the number of affected children appeared to continuously increase over time (2013 [2; 4.4%], 2014 [4; 9.3%], and 2015 [10; 16.4%]). Phylogenetic analysis of the full genome and ORF1, ORF2, and ORF3 nucleotide sequences showed that GII.17 was grouped in cluster III with other strains isolated from 2013 to 2015 and had a different evolutionary history from strains collected in 1978 to 2002 and 2005 to 2009 formed clusters I and II. However, the phylogenetic trees also showed that cluster III was divided into subclusters IIIa (CAU-55 and CAU-85) and IIIb (Kawasaki 2014) (CAU-193, CAU-265, CAU-267, CAU-283, and CAU-289). Comparative analysis of the VP1 capsid protein using 15 complete amino acid sequences from noroviruses isolated from 1978 to 2015 showed 99 amino acid changes. These results could be helpful for epidemiological studies to understand circulating norovirus genotypes in population.

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Section: Methodsmentioning

confidence: 99%

Emergence of Norovirus GII.17 Variants among Children with Acute Gastroenteritis in South Korea

et al. 2016

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Genetic diversity of noroviruses detected in oysters in Jinhae Bay, Korea

Shin

et al. 2013

Food Sci Biotechnol

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Evolutionary Phylodynamics of Korean Noroviruses Reveals a Novel GII.2/GII.10 Recombination Event

2014

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Viral gastroenteritis is the most common causal agent of public health problems worldwide. Noroviruses cause nonbacterial acute gastroenteritis in humans of all ages. In this study, we investigated the occurrence of norovirus infection in children with acute gastroenteritis admitted to university hospitals in South Korea. We also analyzed the genetic diversity of the viruses and identified novel recombination events among the identified viral strains. Of 502 children with acute gastroenteritis admitted to our three hospitals between January 2011 and March 2012, genotyping of human noroviruses was performed in 171 (34%) norovirus-positive samples. Of these samples, 170 (99.5%) were in genogroup II (GII), while only one (0.5%) was in genogroup I (GI). The most common GII strain was the GII.4-2006b variant (n = 96, 56.5%), followed by GII.6 (n = 23, 13.5%), GII.12 (n = 22, 12.9%), GII.3 (n = 20, 11.8%), GII.2 (n = 6, 3.5%), GII.b (n = 2, 1.2%), and GII.10 (n = 1, 0.6%). Potential recombination events (polymerase/capsid) were detected in 39 GII strains (22.9%), and the most frequent genotypes were GII.4/GII.12 (n = 12, 30.8%), GII.4/GII.6 (n = 12, 30.8%), GII.4/GII.3 (n = 8, 20.5%), GII.b/GII.3 (n = 3, 7.7%), GII.16/GII.2 (n = 2, 5.1%), GII.4/GII.2 (n = 1, 2.6%), and GII.2/GII.10 (n = 1, 2.6%). For the first time, a novel GII.2/GII.10 recombination was detected; we also identified the GII.16/GII.2 strain for the first time in South Korea. Our data provided important insights into new recombination events, which may prove valuable for predicting the emergence of circulating norovirus strains with global epidemic potential.

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Genetic diversity of norovirus in outbreaks of gastroenteritis in Seoul

Cited by 3 publications

References 18 publications

Emergence of Norovirus GII.17 Variants among Children with Acute Gastroenteritis in South Korea

Emergence of Norovirus GII.17 Variants among Children with Acute Gastroenteritis in South Korea

Genetic diversity of noroviruses detected in oysters in Jinhae Bay, Korea

Evolutionary Phylodynamics of Korean Noroviruses Reveals a Novel GII.2/GII.10 Recombination Event

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