Genetic diversity of plasmodium falciparum isolates in Minna, North Central Nigeria inferred by PCR genotyping of Merozoite surface protein 1 and 2

Hadijah, Usman-Yamman; Innocent, Omalu Cj; Abdulkadir, A.; Abolarinwa, S.O.; Samuel, E.; Chidiebere, Otuu A

doi:10.1016/j.meegid.2021.105143

Cited by 13 publications

(22 citation statements)

References 27 publications

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“…Another limitation is the small sample size that was found positive for the FC27 gene. In general, infection complexity was moderate to high in the North-West and North-Central in msp2 [15,24,31]. In the South-West, however, the results were mixed, with numerous regions reporting extremely low and very high MOI [25,32,34].…”

Section: Resultsmentioning

confidence: 99%

“…In Niger State, in North-Central Nigeria, Usman-Yamman et al [15] found that msp1 was the most common allele among symptomatic patients, with MAD20 and FC27 as the most common msp1 and msp2 alleles, respectively (Table 1). Other findings in the North-West and North-Central regions contradict this [19,23].…”

Section: Resultsmentioning

confidence: 99%

“…The incidence of RO33 was likewise determined to be mostly a southern phenomenon, with no or few reports in the country's northern regions. Notably, research in the north only found polymorphism RO33 in the presence of other alleles [15], whereas reports from the south found a mix of monomorphic and polymorphic RO33 in single and complex infections [25].…”

Section: Resultsmentioning

confidence: 99%

“…MSP-2 was detected in 31 (64.58%) of the blood samples, with both FC27 and 3D7 allelic types being present. Using MSP 1 and 2 molecular markers, Usman-Yamman et al [15] investigated the genetic diversity among newborns between the ages of 6 months and 17 years. MSP 1's dominant allele was MAD 20, while MSP 2's dominant allele was FC-27.…”

Section: Resultsmentioning

confidence: 99%

“…Several studies have found that the genetic diversity of this parasite varies depending on a variety of parameters, including the degree of infection, the age of the infected persons, and their geographic location [13][14][15][16]. In malaria-endemic countries like Nigeria, where transmission rates are higher, this diversity is greater [17].…”

Section: Role Of Merozoites and Merozoite Surface Proteins (Msps) In ...mentioning

confidence: 99%

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Genetic diversity of Plasmodium falciparum isolates in Nigeria. A review

Opute

Akinkunmi

Funsho

et al. 2022

Egypt J Med Hum Genet

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Background The complexity of infection in malaria-endemic areas is exacerbated by the presence of genetically diverse Plasmodium falciparum strains. There is a risk that more virulent or drug-resistant versions of the disease may arise. Therefore, we reviewed most reported molecular markers that have been detailed to date in Nigeria. Main body of the abstract In this review, we have summarized the genetic diversity of P. falciparum in Nigeria using the two well-reported genes (msp1 and msp2) as genetic diversity biomarkers. The review includes the findings obtained from research conducted in all major geopolitical regions of the country. We found that MSP-2 infection complexity is generally moderate to high in the North-central region. However, in the South-West, there were several regions where the multiplicity of infection (MOI) was either low or extremely high. Conclusion Understanding how Nigeria's malaria situation fits into various reports on P. falciparum genetic variation can improve treatment and immunization options. This review will be helpful for future treatment strategies that would be tailored to the specific needs of Nigeria's malaria-endemic populations.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Role Of Merozoites and Merozoite Surface Proteins (Msps) In ...mentioning

confidence: 99%

See 3 more Smart Citations

Genetic diversity of Plasmodium falciparum isolates in Nigeria. A review

Opute

Akinkunmi

Funsho

et al. 2022

Egypt J Med Hum Genet

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Molecular markers for malaria genetic epidemiology: progress and pitfalls

Ruybal-Pesántez,

McCann,

Vibin

et al. 2024

Trends in Parasitology

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Plasmodium falciparum Merozoite Surface Proteins Polymorphisms and Treatment Outcomes among Patients with Uncomplicated Malaria in Mwanza, Tanzania

Marwa

Lyimo

Konje

et al. 2022

Journal of Tropical Medicine

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Background. The severity of malaria infection depends on the host, parasite and environmental factors. Merozoite surface protein (msp) diversity determines transmission dynamics, P. falciparum immunity evasion, and pathogenesis or virulence. There is limited updated information on P. falciparum msp polymorphisms and their impact on artemether-lumefantrine treatment outcomes in Tanzania. Therefore, this study is aimed at examining msp genetic diversity and multiplicity of infection (MOI) among P. falciparum malaria patients. The influence of MOI on peripheral parasite clearance and adequate clinical and parasitological response (ACPR) was also assessed. Methods. Parasite DNA was extracted from dried blood spots according to the manufacture’s protocol. Primary and nested PCR were performed. The PCR products for both the block 2 region of msp1 and the block 3 regions of msp2 genes and their specific allelic families were visualized on a 2.5% agarose gel. Results. The majority of the isolates, 58/102 (58.8%) for msp1 and 69/115 (60.1%) for msp2, harboured more than one parasite genotypes. For the msp1 gene, K1 was the predominant allele observed (75.64%), whereas RO33 occurred at the lowest frequency (43.6%). For the msp2 gene, the 3D7 allele was observed at a higher frequency (81.7%) than the FC27 allele (76.9%). The MOIs were 2.44 for msp1 and 2.27 for msp2 ( p = 0.669 ). A significant correlation between age and multiplicity of infection (MOI) for msp1 or MOI for msp2 was not established in this study (rho = 0.074, p = 0.521 and rho = −0.129, p = 0.261 , respectively). Similarly, there was no positive correlation between parasite density at day 1 and MOI for both msp1 (rho = 0.113, p = 0.244 ) and msp2 (rho = 0.043, p = 0.712 ). The association between MOI and ACPR was not observed for either msp1 or mps2 ( p = 0.776 and 0.296, respectively). Conclusions. This study reports high polyclonal infections, MOI and allelic frequencies for both msp1 and msp2. There was a lack of correlation between MOI and ACPR. However, a borderline significant correlation was observed between day 2 parasitaemia and MOI.

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Genetic diversity of plasmodium falciparum isolates in Minna, North Central Nigeria inferred by PCR genotyping of Merozoite surface protein 1 and 2

Cited by 13 publications

References 27 publications

Genetic diversity of Plasmodium falciparum isolates in Nigeria. A review

Genetic diversity of Plasmodium falciparum isolates in Nigeria. A review

Molecular markers for malaria genetic epidemiology: progress and pitfalls

Plasmodium falciparum Merozoite Surface Proteins Polymorphisms and Treatment Outcomes among Patients with Uncomplicated Malaria in Mwanza, Tanzania

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