Genetic diversity of Plasmodium falciparum in human malaria cases in Mali

Nabet, Cécile; Doumbo, Safiatou; Jeddi, Fakhri; Konaté, Salimata; Manciulli, Tommaso; Fofana, Bakary; L’Ollivier, Coralie; Camara, Aminata; Moore, Sandra; Ranque, Stéphane; Théra, Mahamadou A.; Doumbo, Ogobara K.; Piarroux, Renaud

doi:10.1186/s12936-016-1397-0

Cited by 42 publications

(62 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This variability in parasite population genetics observed between studies sites is consistent with the difference in malaria incidence in these two regions [2]. These ndings suggest that the genetic diversity of P. falciparum is greater in high malaria transmission areas and decreases when transmission regresses [2,4]. The neighbour-joining tree of different allele types revealed two parasite clusters in the South as in the North and many clones sharing same patterns in amino acid level showing parental link between strains.…”

Section: Discussionsupporting

confidence: 79%

“…A better understanding of malaria epidemiology could be helpful for disease control and prevention. The extent of genetic diversity and multiplicity of infection (MOI) are essential to understand malaria epidemiological patterns, providing insight into the dynamics of malaria transmission, human exposure to mosquito bites, acquisition of malaria immunity [2][3][4][5] and assessing of malaria control interventions [6][7][8].…”

mentioning

confidence: 99%

See 1 more Smart Citation

Molecular Epidemiology of Plasmodium Falciparum by Multiplexed Amplicon Deep Sequencing in Senegal

Ndiaye

Gaye

et al. 2020

Preprint

View full text Add to dashboard Cite

Background Molecular epidemiology can provide important information regarding the genetic diversity and transmission of Plasmodium falciparum, which can assist in designing and monitoring elimination efforts. However, malaria molecular epidemiology including understanding the genetic diversity of the parasite and performing molecular surveillance of transmission has been poorly documented in Senegal. Next Generation Sequencing (NGS) offers a practical, fast and high-throughput approach to understand malaria population genetics. This study aims to unravel the population structure of P. falciparum and to estimate the allelic diversity, multiplicity of infection (MOI), and evolutionary patterns of the malaria parasite using the NGS platform. Methods Multiplex amplicon deep sequencing of merozoite surface protein 1 (PfMSP1) and merozoite surface protein 2 (PfMSP2) genes in fifty-three P. falciparum isolates from two epidemiologically different areas in the South and North of Senegal, was carried out. Results A total of 76 Pfmsp1 and 116 Pfmsp2 clones were identified and 135 different alleles were found, 56 and 79 belonged to the pfmsp1 and pfmsp2 genes, respectively. K1 and IC3D7 allelic families were most predominant in both sites. The local haplotype diversity (Hd) and nucleotide diversity (π) were higher in the South than in the North for both genes. For pfmsp1, a high positive Tajima’s D (TD) value was observed in the South (D = 2.0453) while negative TD value was recorded in the North (D=-1.46045) and F-Statistic (Fst) was 0.19505. For pfmsp2, non-directional selection was found with a highly positive TD test in both areas and Fst was 0.02111. The mean MOI for both genes was 3.07 and 1.76 for the South and the North, respectively, with a statistically significant difference between areas (p = 0.001). Conclusion This study revealed a high genetic diversity of pfmsp1 and pfmsp2 genes and low genetic differentiation in P. falciparum population in Senegal. The MOI means were significantly different between the Southern and Northern areas. Findings also showed that multiplexed amplicon deep sequencing is a useful technique to investigate genetic diversity and molecular epidemiology of P. falciparum infections.

show abstract

Section: Discussionsupporting

confidence: 79%

mentioning

confidence: 99%

Molecular Epidemiology of Plasmodium Falciparum by Multiplexed Amplicon Deep Sequencing in Senegal

Ndiaye

Gaye

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…This evidence was generated by genotyping two-thirds of all documented, symptomatic and asymptomatic P. falciparum infections in the country over the span of two years, thus represents a comprehensive picture of the parasite population. The genetic diversity and large effective population size reveal a P. falciparum population similar to those observed among high transmission regions of sub-Saharan Africa [14][15][16][17]42]. Importantly, imported and local cases had similar levels of population-level diversity, and though MOI and F WS were higher among imported cases, the magnitude of these differences did not allow for accurate discrimination between these two groups.…”

Section: Discussionmentioning

confidence: 88%

“…Thailand [20], Vietnam [37], Honduras [38], and Indonesia [39]) and was more comparable to that of high transmission countries (e.g. Guinea [16], The Gambia [14], and Mali [15]) ( Table 3).…”

Section: Genetic Diversity Of Parasites In Eswatini Is Similar To Higmentioning

confidence: 85%

“…This "genomic thermometer" framework has been based on numerous studies that have shown that P. falciparum parasite populations generally have higher population and within-host diversity (i.e. higher multiplicity of infection or MOI) in high transmission settings of sub-Saharan Africa than in lower transmission settings such as Southeast Asia and Latin America [8,11,[14][15][16][17][18][19][20][21][22]. However, studies of P. falciparum parasite diversity in low transmission settings of sub-Saharan Africa are limited, and in these settings, importation of malaria from neighboring, high transmission countries may complicate the relationship between transmission intensity and genetic diversity.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

High genetic diversity of Plasmodium falciparum in the low transmission setting of the Kingdom of Eswatini

Roh

Tessema

Murphy

et al. 2019

Preprint

View full text Add to dashboard Cite

show abstract

Plasmodium falciparum genetic diversity; implications for malaria control in Ethiopia: Systematic review and meta‐analysis

Abriham,

Belew,

Baffa

et al. 2024

Health Science Reports

View full text Add to dashboard Cite

BackgroundIn malaria endemic regions, Plasmodium falciparum infection is characterized by variable genetic diversity at different settings. The parasite's various forms are found at varied frequency in different geographic areas. Understanding malaria parasite diversity and transmission is vital to evaluate control interventions. The aim of this study was under taken to determine the status of P. falciparum genetic diversity and MOI in different regions of Ethiopia.MethodsRelevant publications were identified from electronic databases such as; PubMed, EMBASE, Google scholar and Google. Besides, an online search was done using the above databases for all articles published in English on genetic diversity of P. falciparum in Ethiopia. STATA software was used for data analysis. The pooled estimates were calculated using random effect model. The summary estimates were presented using forest plots and tables.ResultsA total of 11 studies were included in the systematic review. However, only 8, 10 and 2 studies were included for Pfmsp‐1, Pfmsp‐2 and glurp gene meta‐analysis, respectively. However, the meta‐analysis result showed that the pooled prevalence of Pfmsp‐1, msp‐2 and glurp gene were 84% for both msp‐1/2% and 51%, respectively. The pooled prevalence of msp‐1 gene was higher in Amhara followed by Oromia region and lower in SNNPR while, for msp‐2 gene the pooled prevalence was higher in Benshangul gumez region. Among the allelic family of msp‐1 and msp‐2 genes, MAD20 (34%) and FC27 (44%) were the most predominant respectively.ConclusionBased on the review, there is evidence of the presence of high genetic diversity of P. falciparum parasites in Ethiopia, suggesting that malaria transmission remain high and that strengthened control efforts are needed. The approaches and methods used for investigation of diversified parasites have similarity between studies and should use advanced molecular techniques, like microsatellite, to assess the genetic diversity of P. falciparum for better results.

show abstract

Genetic diversity of Plasmodium falciparum in human malaria cases in Mali

Cited by 42 publications

References 34 publications

Molecular Epidemiology of Plasmodium Falciparum by Multiplexed Amplicon Deep Sequencing in Senegal

Molecular Epidemiology of Plasmodium Falciparum by Multiplexed Amplicon Deep Sequencing in Senegal

High genetic diversity of Plasmodium falciparum in the low transmission setting of the Kingdom of Eswatini

Plasmodium falciparum genetic diversity; implications for malaria control in Ethiopia: Systematic review and meta‐analysis

Contact Info

Product

Resources

About