Genetic diversity of Plasmodium vivax malaria in China and Myanmar

Zhong, Daibin; Bonizzoni, Mariangela; Zhou, Guofa; Wang, Guangze; Chen, Bin; Vardo-Zalik, Anne M.; Yan, Guiyun; Zheng, Bin

doi:10.1016/j.meegid.2011.05.009

Cited by 29 publications

(39 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Seven patterns were detected in the pvmsp-3α gene after digestion of the PCR products with HhaI , with the most frequent allele variant being PH1 subtype. The results were consistent with those reported from other regions of the world, including those from Myanmar (n = 14) [7], Iran (n = 6) [14], Colombia (n = 9) [22], French Guiana (n = 11) [23], Thailand (n = 6) [28], Venezuela (n = 9) [29], and Peru (n = 17) [30]. However, outside Anhui province, the allele PH3, the second most common one in the present study, was the most prevalent in other areas of China including Hainan and Yunnan provinces, and also in Myanmar [7].…”

Section: Discussionsupporting

confidence: 92%

Genetic diversity of Plasmodium vivax population in Anhui province of China

Huang

et al. 2014

Malar J

View full text Add to dashboard Cite

BackgroundAlthough the numbers of malaria cases in China have been declining in recent years, outbreaks of Plasmodium vivax malaria were still being reported in rural areas south of the Yellow River. To better understand the transmission dynamics of P. vivax parasites in China, the extent of genetic diversity of P. vivax populations circulating in Bozhou of Anhui province of China were investigated using three polymorphic genetic markers: merozoite surface proteins 1 and 3α (pvmsp-1 and pvmsp-3α) and circumsporozoite protein (pvcsp).MethodsForty-five P. vivax clinical isolates from Bouzhou of Anhui province were collected from 2009 to 2010 and were analysed using PCR/RFLP or DNA sequencing.ResultsSeven and six distinct allelic variants were identified using PCR/RFLP analysis of pvmsp-3α with HhaI and AluI, respectively. DNA sequence analysis of pvmsp-1 (variable block 5) revealed that there were Sal-I and recombinant types but not Belem type, and seven distinct allelic variants in pvmsp-1 were detected, with recombinant subtype 2 (R2) being predominant (66.7%). All the isolates carried pvcsp with VK210 type but not VK247 or P. vivax-like types in the samples. Sequence analysis of pvcsp gene revealed 12 distinct allelic variants, with VK210-1 being predominant (41.5%).ConclusionsThe present data indicate that there is some degree of genetic diversity among P. vivax populations in Anhui province of China. The genetic data obtained may assist in the surveillance of P. vivax infection in endemic areas or in tracking potential future disease outbreak.

show abstract

Section: Discussionsupporting

confidence: 92%

Genetic diversity of Plasmodium vivax population in Anhui province of China

Huang

et al. 2014

Malar J

View full text Add to dashboard Cite

show abstract

“…The reasons for the serious malaria epidemics in Yunnan Province may include its muggy tropical and subtropical climate, especially in tropical valleys [8, 9], and social factors such as the international movement of individuals to and from Laos, Myanmar and Vietnam. The trans-national population movement has been a major obstacle of effective malaria prevention and repetitive epidemics [10, 11]. …”

Section: Introductionmentioning

confidence: 99%

Spatiotemporal epidemic characteristics and risk factor analysis of malaria in Yunnan Province, China

Yang

Wang

et al. 2017

BMC Public Health

View full text Add to dashboard Cite

BackgroundMalaria remains an important public health concern in China and is particularly serious in Yunnan, a China’s provincial region of high malaria burden with an incidence of 1.79/105 in 2012. This study aims to examine the epidemiologic profile and spatiotemporal aspects of epidemics of malaria, and to examine risk factors which may influence malaria epidemics in Yunnan Province.MethodsThe data of malaria cases in 2012 in 125 counties of Yunnan Province was used in this research. The epidemical characteristics of cases were revealed, and time and space clusters of malaria were detected by applying scan statistics method. In addition, we applied the geographically weighted regression (GWR) model in identifying underlying risk factors.ResultsThere was a total of 821 cases of malaria, and male patients accounted for 83.9% (689) of the total cases. The incidence in the group aged 20–30 years was the highest, at 3.00/105. The majority (84.1%) of malaria cases occurred in farmers and migrant workers, according to occupation statistics. On a space-time basis, epidemics of malaria of varying severity occurred in the summer and autumn months, and the high risk regions were mainly distributed in the southwest counties. Annual average temperature, annual cumulative rainfall, rice yield per square kilometer and proportion of rural employees mainly showed a positive association with the malaria incidence rate, according to the GWR model.ConclusionsMalaria continues to be one of serious public health issues in Yunnan Province, especially in border counties in southwestern Yunnan. Temperature, precipitation, rice cultivation and proportion of rural employees were positively associated with malaria incidence. Individuals, and disease prevention and control departments, should implement more stringent preventative strategies in locations with hot and humid environmental conditions to control malaria.

show abstract

“…7,8 Previous studies have shown high genetic diversity but limited MOI among P. vivax infections in Southeast Asia. [9][10][11][12] However, the genotyping methods used in these studies are often limited in their ability to detect minority clones, leading to an underestimation of MOI. 8 The heteroduplex tracking assay (HTA) is a genotyping technique that has proven adept at uncovering minority variants within malaria infections and in infections with other agents.…”

Section: Introductionmentioning

confidence: 99%

Plasmodium vivax Isolates from Cambodia and Thailand Show High Genetic Complexity and Distinct Patterns of P. vivax Multidrug Resistance Gene 1 (pvmdr1) Polymorphisms

Lin¹,

Patel²,

Kharabora³

et al. 2013

The American Society of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

Abstract. Plasmodium vivax accounts for an increasing fraction of malaria infections in Thailand and Cambodia. We compared P. vivax genetic complexity and antimalarial resistance patterns in the two countries. Use of a heteroduplex tracking assay targeting the merozoite surface protein 1 gene revealed that vivax infections in both countries are frequently polyclonal (84%), with parasites that are highly diverse (H E = 0.86) but closely related (G ST = 0.18). Following a history of different drug policies in Thailand and Cambodia, distinct patterns of antimalarial resistance have emerged: most Cambodian isolates harbor the P. vivax multidrug resistance gene 1 ( pvmdr1) 976F mutation associated with chloroquine resistance (89% versus 8%, P 0.001), whereas Thai isolates more often display increased pvmdr1 copy number (39% versus 4%, P 0.001). Finally, genotyping of paired isolates from individuals suspected of suffering relapse supports a complex scheme of relapse whereby recurrence of multiple identical variants is sometimes accompanied by the appearance of novel variants.

show abstract

Genetic diversity of Plasmodium vivax malaria in China and Myanmar

Cited by 29 publications

References 47 publications

Genetic diversity of Plasmodium vivax population in Anhui province of China

Genetic diversity of Plasmodium vivax population in Anhui province of China

Spatiotemporal epidemic characteristics and risk factor analysis of malaria in Yunnan Province, China

Plasmodium vivax Isolates from Cambodia and Thailand Show High Genetic Complexity and Distinct Patterns of P. vivax Multidrug Resistance Gene 1 (pvmdr1) Polymorphisms

Contact Info

Product

Resources

About