Genetic diversity of Plasmodium vivax in Kolkata, India

Kim, Jung-Ryong; Imwong, Mallika; Nandy, Abhishek; Chotivanich, Kesinee; Nontprasert, Apichart; Tonomsing, Naowarat; Maji, Ardhendu Kumar; Addy, Manjulika; Day, Nick; White, Nicholas J.; Pukrittayakamee, Sasithon

doi:10.1186/1475-2875-5-71

Cited by 81 publications

(57 citation statements)

References 40 publications

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“…For example, in 90 P. vivax isolates from Thailand, Cui et al (2003b) found a multiple-clone infection rate of 25.6% using the CSP gene alone, 19.3% using MSP-3α alone and 35.6% when the two markers were combined. A similar trend was observed in the analysis of 141 isolates from India described by Kim et al (2006).…”

Section: Varying Detection Methodssupporting

confidence: 65%

A closer look at multiple-clone Plasmodium vivax infections: detection methods, prevalence and consequences

Havryliuk

Ferreira

2009

Mem. Inst. Oswaldo Cruz

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Section: Varying Detection Methodssupporting

confidence: 65%

A closer look at multiple-clone Plasmodium vivax infections: detection methods, prevalence and consequences

Havryliuk

Ferreira

2009

Mem. Inst. Oswaldo Cruz

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“…As a consequence, the number of informative, alignable sites for determining the genetic distance and diversity among isolates was much less than the gene length for most isolates (Table 1). As observed before (e.g., [10,18]), there were numerous sequences with large (over 300 bp) deletion mutations relative to the Salvador-1 reference strain in the N-terminal half of the gene. However, rather than being due to a single, shared deletion event, such shorter alleles were the result of multiple combinations of indels.…”

Section: Resultssupporting

confidence: 63%

Merozoite surface protein-3 alpha as a genetic marker for epidemiologic studies in Plasmodium vivax: a cautionary note

Rice

Acosta

Pacheco

et al. 2013

Malar J

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BackgroundPlasmodium vivax is the most widespread of the human malaria parasites in terms of geography, and is thought to present unique challenges to local efforts aimed at control and elimination. Parasite molecular markers can provide much needed data on P. vivax populations, but few such markers have been critically evaluated. One marker that has seen extensive use is the gene encoding merozoite surface protein 3-alpha (MSP-3α), a blood-stage antigen known to be highly variable among P. vivax isolates. Here, a sample of complete msp-3α gene sequences is analysed in order to assess its utility as a molecular marker for epidemiologic investigations.MethodsAmplification, cloning and sequencing of additional P. vivax isolates from different geographic locations, including a set of Venezuelan field isolates (n = 10), yielded a sample of 48 complete msp-3α coding sequences. Characterization of standard population genetic measures of diversity, phylogenetic analysis, and tests for recombination were performed. This allowed comparisons to patterns inferred from the in silico simulation of a polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) protocol used widely.ResultsThe larger sample of MSP-3α diversity revealed incongruence between the observed levels of nucleotide polymorphism, which were high in all populations, and the pattern of PCR-RFLP haplotype diversity. Indeed, PCR-RFLP haplotypes were not informative of a population’s genetic diversity and identical haplotypes could be produced from analogous bands in the commonly used protocol. Evidence of frequent and variable insertion-deletion mutations and recurrent recombination between MSP-3α haplotypes complicated the inference of genetic diversity patterns and reduced the phylogenetic signal.ConclusionsThe genetic diversity of P. vivax msp-3α involves intragenic recombination events. Whereas the high genetic diversity of msp-3α makes it a promising marker for some epidemiological applications, the ability of msp-3α PCR-RFLP analysis to accurately track parasites is limited. Local studies of the circulating alleles are needed before implementing PCR-RFLP approaches. Furthermore, evidence from the global sample analysed here suggests such msp-3α PCR-RFLP methods are not suitable for broad geographic studies or tracking parasite populations for an extended period of time.

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“…Furthermore, the vk247 genotype displays high identity at the carboxyl end to the csp sequence reported for Plasmodium cynomolgi (Gen Bank AB524342.1). This contrasts with the higher rat diversity of vk210 than vk247 CRRs, possibly related to the higher worldwide prevalence of vk210 than vk247 [14,15,20,30,47,58,70-74]. In Southern Mexico vk247 became more prevalent from 1997 onwards [19,28].…”

Section: Discussionmentioning

confidence: 99%

“…However, more extensive genome sequencing analysis is required for a better insight into parasite evolution and its dispersal into Meso and South America. The marked genetic structure determined in LA regions, but the microheterogeneity [11,64,67,74,75] revealed the presence of scenarios epidemiologically diverse. P. vivax genetics and biological characteristics, host immunity and local vectors may contribute to their different patterns of demographic expansion favoured or limited by the eco-epidemiological conditions.…”

Section: Discussionmentioning

confidence: 99%

Molecular epidemiology of Plasmodium vivax in Latin America: polymorphism and evolutionary relationships of the circumsporozoite gene

González-Cerón

Martínez-Barnetche

Montero‐Solis

et al. 2013

Malar J

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BackgroundThe origins and dispersal of Plasmodium vivax to its current worldwide distribution remains controversial. Although progress on P. vivax genetics and genomics has been achieved worldwide, information concerning New World parasites remains fragmented and largely incomplete. More information on the genetic diversity in Latin America (LA) is needed to better explain current patterns of parasite dispersion and evolution.MethodsPlasmodium vivax circumsporozoite protein gene polymorphism was investigated using polymerase chain reaction amplification and restriction fragment length polymorphism (PCR-RFLP), and Sanger sequencing in isolates from the Pacific Ocean coast of Mexico, Nicaragua, and Peru. In conjunction with worldwide sequences retrieved from the Genbank, mismatch distribution analysis of central repeat region (CRR), frequency estimation of unique repeat types and phylogenetic analysis of the 3′ terminal region, were performed to obtain an integrative view of the genetic relationships between regional and worldwide isolates.ResultsFour RFLP subtypes, vk210a, b, c and d were identified in Southern Mexico and three subtypes vk210a, e and f in Nicaragua. The nucleotide sequences showed that Mexican vk210a and all Nicaraguan isolates were similar to other American parasites. In contrast, vk210b, c and d were less frequent, had a domain ANKKAEDA in their carboxyl end and clustered with Asian isolates. All vk247 isolates from Mexico and Peru had identical RFLP pattern. Their nucleotide sequences showed two copies of GGQAAGGNAANKKAGDAGA at the carboxyl end. Differences in mismatch distribution parameters of the CRR separate vk247 from most vk210 isolates. While vk247 isolates display a homogeneous pattern with no geographical clustering, vk210 isolates display a heterogeneous geographically clustered pattern which clearly separates LA from non-American isolates, except vk210b, c and d from Southern Mexico.ConclusionsThe presence of vk210a in Mexico and vk210e, f and g in Nicaragua are consistent with other previously reported LA isolates and reflect their circulation throughout the continent. The vk210b, c and d are novel genotypes in LA. Their genetic relationships and low variability within these vk210 and/or within the vk247 parasites in Southern Mexico suggest its recent introduction and/or recent expansion to this region. The global analysis of P. vivax csp suggests this parasite introduction to the region and likely LA by different independent events.

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Genetic diversity of Plasmodium vivax in Kolkata, India

Cited by 81 publications

References 40 publications

A closer look at multiple-clone Plasmodium vivax infections: detection methods, prevalence and consequences

A closer look at multiple-clone Plasmodium vivax infections: detection methods, prevalence and consequences

Merozoite surface protein-3 alpha as a genetic marker for epidemiologic studies in Plasmodium vivax: a cautionary note

Molecular epidemiology of Plasmodium vivax in Latin America: polymorphism and evolutionary relationships of the circumsporozoite gene

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