Genetic diversity of Pseudo-nitzschia brasiliana (Bacillariophyceae) from Malaysia

Lim, Hong-Chang; Lim, Po Teen; Su, Suriyanti Nyun-Pau; Teng, Sing Tung

doi:10.1007/s10811-012-9803-7

Cited by 20 publications

(19 citation statements)

References 40 publications

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“…, Lim et al. ). Numerical and statistical descriptions of the secondary structure information are given in Table S5 in the Supporting Information.…”

Section: Resultsmentioning

confidence: 99%

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Three novel species in the Pseudo‐nitzschia pseudodelicatissima complex: P. batesiana sp. nov., P. lundholmiae sp. nov., and P. fukuyoi sp. nov. (Bacillariophyceae) from the Strait of Malacca, Malaysia

Lim

Teng

Leaw

et al. 2013

Journal of Phycology

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A study on the morphology and phylogeny of 18 strains of Pseudo-nitzschia established from the Strait of Malacca, Peninsular Malaysia, was undertaken. Morphological data combined with molecular evidence show that they constitute three new species, for which the names, P. batesiana sp. nov., P. lundholmiae sp. nov., and P. fukuyoi sp. nov., are proposed. The three new species closely resemble species in the P. pseudodelicatissima complex sensu lato. Morphologically, P. batesiana differs from other species in the complex by having a smaller part of cell overlapping in the chain, whereas P. lundholmiae differs by having fewer poroid sectors and P. fukuyoi by having a distinct type of poroid sectors. Nucleotide sequences of the LSU rDNA (D1-D3) of the three new species reveal significant nucleotide sequence divergence (0.1%-9.3%) from each other and from other species in the P. pseudodelicatissima complex s.l. The three species are phylogenetically closely related to species in the P. pseudodelicatissima complex, with P. batesiana appearing as a sister taxon to P. circumpora, P. caciantha, and P. subpacifica; whereas P. lundholmiae and P. fukuyoi are more closely related to P. pseudodelicatissima and P. cuspidata. The three species show 2-3 compensatory base changes (CBCs) in their ITS2 transcripts when compared to the closely related species. The ITS2 with its structural information has proven its robustness in constructing a better resolved phylogenetic framework for Pseudo-nitzschia.

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“…, Lim et al. ). Numerical and statistical descriptions of the secondary structure information are given in Table S5 in the Supporting Information.…”

Section: Resultsmentioning

confidence: 99%

“…, Lim et al. ). The LSU rDNA in the domain 1–3 (D1–D3) was amplified using the primer pair, D1R and D3Ca (Scholin et al.…”

Section: Methodsmentioning

confidence: 99%

Three novel species in the Pseudo‐nitzschia pseudodelicatissima complex: P. batesiana sp. nov., P. lundholmiae sp. nov., and P. fukuyoi sp. nov. (Bacillariophyceae) from the Strait of Malacca, Malaysia

Lim

Teng

Leaw

et al. 2013

Journal of Phycology

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“…Pseudo‐nitzschia brasiliana and P. pungens are reported as widely distributed in Malaysian coastal waters (Lim et al. , ,b, , Su ). This present study records for the first time the occurrence of P. cuspidata , P. dolorosa , and P. circumpora in Malaysian waters.…”

Section: Discussionmentioning

confidence: 99%

MORPHOLOGY AND MOLECULAR CHARACTERIZATION OF PSEUDO‐NITZSCHIA (BACILLARIOPHYCEAE) FROM MALAYSIAN BORNEO, INCLUDING THE NEW SPECIES PSEUDO‐NITZSCHIA CIRCUMPORA SP. NOV

Lim

Leaw

et al. 2012

Journal of Phycology

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Field sampling was undertaken to investigate the occurrence of Pseudo-nitzschia Peragallo species in eight locations along the coast of Malaysian Borneo. A total of 108 strains of Pseudo-nitzschia species were isolated, and their morphology examined with SEM and TEM. Additionally, molecular data from nuclear-encoded partial LSU rDNA, and ITS regions, were characterized. A total of five species were confidently identified based on a combination of distinct morphological characteristics and supporting molecular evidence: P. brasiliana Lundholm, Hasle & Fryxell, P. cuspidata (Hasle) Hasle, P. dolorosa Lundholm & Moestrup, P. micropora Priisholm, Moestrup & Lundholm, and P. pungens (Grunow) Hasle var. pungens. However, one morphotype from Sarawak, while somewhat similar to P. caciantha, showed significant morphological distinction from this and any other of the currently described species. Most notably this morphotype possessed a characteristic pore arrangement in the poroids, with the fine pores in each perforation sector arranged in circles. Pair-wise sequence comparison of the LSU rDNA between this unidentified morphotype and P. caciantha Lundholm, Moestrup & Hasle, revealed 2.7% genetic divergence. Phylogenetic analyses strongly supported the monophyly of the morphotype. Based upon these supporting data it is here described as a new species, Pseudo-nitzschia circumpora sp. nov. A key to the six species of Pseudo-nitzschia from Malaysian Borneo is presented. Molecular signatures for all species were established based on structural comparisons of ITS2 rRNA transcripts.

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“…Genomic DNAs obtained from the previous studies [6][7][8]15] were also used in the present work for gene amplifications. Gene amplification was performed using an Eppendorf gradient thermocycler (Eppendorf, Germany).…”

Section: Dna Extraction Amplification and Sequencingmentioning

confidence: 99%

“…Molecular species characterization using nucleotide sequences of the nuclearencoded large-subunit (LSU) ribosomal DNA (rDNA) and the internal transcribed spacer region (ITS1-5.8S-ITS2) have become essential supporting criteria in species delineation [9][10][11][12][13][14][15][16]. Other molecular techniques used in Pseudonitzschia studies included automated ribosomal intergenic spacer analysis (ARISA) [17][18][19]; rapid whole-cell detection using fluorescence in situ hybridization (FISH) [12,[20][21][22][23]; and the development of species-specific microsatellite markers for population genetics [24,25].…”

Section: Introductionmentioning

confidence: 99%

Pseudo-nitzschia species (Bacillariophyceae) identification and delineation using mitochondrial cox1 gene sequences as compared to LSU rDNA

et al. 2015

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Genetic diversity of Pseudo-nitzschia brasiliana (Bacillariophyceae) from Malaysia

Cited by 20 publications

References 40 publications

Three novel species in the Pseudo‐nitzschia pseudodelicatissima complex: P. batesiana sp. nov., P. lundholmiae sp. nov., and P. fukuyoi sp. nov. (Bacillariophyceae) from the Strait of Malacca, Malaysia

Three novel species in the Pseudo‐nitzschia pseudodelicatissima complex: P. batesiana sp. nov., P. lundholmiae sp. nov., and P. fukuyoi sp. nov. (Bacillariophyceae) from the Strait of Malacca, Malaysia

MORPHOLOGY AND MOLECULAR CHARACTERIZATION OF PSEUDO‐NITZSCHIA (BACILLARIOPHYCEAE) FROM MALAYSIAN BORNEO, INCLUDING THE NEW SPECIES PSEUDO‐NITZSCHIA CIRCUMPORA SP. NOV

Pseudo-nitzschia species (Bacillariophyceae) identification and delineation using mitochondrial cox1 gene sequences as compared to LSU rDNA

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