Genetic Diversity of Rabbit (Oryctolagus cuniculus) Population in South Eastern Nigeria Using Microsatellite Markers

Adeolu, A. I.; Wheto, Mathew; Oleforuh-Okoleh, V. U.; Nwose, R.N.; Adenaike, A. S.; Yakubu, Abdulmojeed; Abiola, E. M.; Mohammed, Benyarou

doi:10.5398/tasj.2021.44.3.280

Cited by 5 publications

(1 citation statement)

References 10 publications

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“…The study of rabbit genetic diversity was widely achieved by many molecular markers like RAPD and SRAP 21 ; the mitochondrial DNA 22 ; simple sequence repeat (SSR) microsatellites 23 ; and single-nucleotide polymorphisms (SNPs), 24 .…”

Section: Discussionmentioning

confidence: 99%

Molecular genetic variations of some rabbit breeds using small mitochondrial rRNA sequences

Allam,

Al-Farga,

Wlson

2024

Preprint

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With the help of small mitochondrial rRNA (12S rRNA), the genetic diversity of five different rabbit breeds in Egypt was estimated in the current study. Slight difference was found between 12S rRNA sequences of the five different rabbit breeds 923 bp to 924 bp. The final alignments consisted of 924 bp., of them 913 conserved sites. The accession numbers for these sequences in GenBank/NCBI are (OR210201-OR210205). All breeds had an average A + T rating of 59.68%. The genetic distance values amongst breeds extended from 0.000 to 0.0033. The (0.0033) percentage was observed amongst the breed New Zealand White and both Baladi Black and REX breeds. As well as low genetic similarity was shown between New Zealand White breed and the rest breeds. The information provided by the 12S rRNA sequences demonstrated that the New Zealand White breed was genetically unique from the other breeds. The investigation's findings validate the suitability and qualification of 12S rRNA for observing breed-specific genetic variation in rabbits. The generation of genetic maintenance and amelioration strategies for Egyptian rabbit genetic resources may benefit from this data in the future.

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Section: Discussionmentioning

confidence: 99%

Molecular genetic variations of some rabbit breeds using small mitochondrial rRNA sequences

Allam,

Al-Farga,

Wlson

2024

Preprint

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Genetic Variation and Phylogenetic relationships of Commercial Pigs and Indigenous Pigs in Southwestern Nigeria

Oluwole

Edward²,

Adeola³

et al. 2022

Preprint

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This study examined genetic variations among indigenous and commercial pigs in Southwestern Nigeria. Sixty (60) individuals including Nigerian indigenous pigs (NIP) (n = 25), exotic (n = 25), and crosses between the exotic pigs (n = 10) were genotyped based on eight microsatellite markers. The mean number of alleles (Na) observed in the overall population of the three populations was 3.63 ± 0.36. Allele frequency ranged from 0.00 to 1.00 from the 39 alleles obtained within the population. Other allele frequency results obtained from the interpretation for the microsatellite loci across the three populations were polymorphic except for S0101 that was monomorphic. The mean number of effective allele (Ne) was 2.22 ± 0.17. The means obtained in this study for heterozygosities (Ho and He) were 0.529 and 0.501 respectively and the F-statistics showed the reduction in heterozygosity at various loci across the population studied i.e FIS and FIT. The mean values for FIS and FIT were − 0.06 ± 0.08 and − 0.02 ± 0.08, respectively. The mean level of gene flow (Nm) among the population was estimated to be 9.18 ± 3.59. The pairwise FST values among the three populations ranged from 0.02 to 0.04. The very low genetic differentiation between NIP and other populations reflects high gene flow. Molecular variance analysis revealed that there were 2% variance among the populations, 48% among the individual and 51% within the populations, with estimated variance of 0.04, 1.24 and 1.31 respectively. The phylogenetic tree separated the NIP from the remaining two populations while the exotic and the crosses clustered closely. This study revealed that genomes of pure indigenous pigs in Southwestern Nigeria are threatened by genetic erosion and recommend quick actions for sustainable conservation strategies.

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Microsatellite markers application in the genetic survey of native rabbits in the Egyptian delta

Emam,

Makhlouf,

Mourad

2024

Genetika

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The human interest in exotic animal breeds in the agricultural sector led to the deterioration of local breeds. The interest in national farm animal genetic studies is important for the agriculture ecosystems under climate change challenges. Microsatellite markers are important tools to determine the genetic status of breeds, populations, and subpopulations. In this study, 28 microsatellite loci were used to investigate the genetic situation among 274 biological samples collected from the native Delta Egypt rabbits (NDER) population in the north of Egypt. They belonged to eight subpopulations (Damietta, Dakahlia, Kafr El sheikh, Beheira, Gharbia, Menoufia, Sharqia, and Qalyubia). It was found that expected heterozygosity (He) values were greater than observed heterozygosity (Ho). A total of 184 alleles were identified, with a mean of 6.571 and 4.122 as effective alleles. About 89% of microsatellite markers expressed high informative values in the polymorphism information content (PIC). The comparison among 8 NDER subpopulations showed low genetic variability parameters with high inbreeding coefficient (FIS) values in the north (Damietta, Dakahlia, Kafr El sheikh, Beheira, and Gharbia). However, values of genetic variables increased with decreasing FIS in the middle (Menoufia), east (Sharqia), and south (Qalyubia) Delta. Furthermore, the discriminant analysis principal components (DAPC) showed overlaying in the north. In the same context, the neighbor-joining tree (NJ) and heatmap showed the genetic convergence among the northern subpopulations. The analysis of STRUCTURE found 4 clusters (K= 8). The north subpopulations were in one cluster, while others in the middle, east, and south were a separate cluster for each subpopulation. Our findings show that the NDER suffers from genetic drift in the northern Delta subpopulations. On the contrary, the south, east, and middle subpopulations showed more genetic variability. A strategy of correct mating should be fostered to improve the genetic traits of rabbits.

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Genetic Diversity of Rabbit (Oryctolagus cuniculus) Population in South Eastern Nigeria Using Microsatellite Markers

Cited by 5 publications

References 10 publications

Molecular genetic variations of some rabbit breeds using small mitochondrial rRNA sequences

Molecular genetic variations of some rabbit breeds using small mitochondrial rRNA sequences

Genetic Variation and Phylogenetic relationships of Commercial Pigs and Indigenous Pigs in Southwestern Nigeria

Microsatellite markers application in the genetic survey of native rabbits in the Egyptian delta

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