Genetic diversity of recent bovine viral diarrhoea viruses from the southeast of Austria (Styria)

Vilček, Štefan; Greiser‐Wilke, I.; Durkovic, B.; Obritzhauser, W.; Deutz, A.; Köfer, J.

doi:10.1016/s0378-1135(02)00296-1

Cited by 31 publications

(20 citation statements)

References 28 publications

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“…It has been previously reported that BVDV-1 is distributed worldwide in comparison to BVDV-2, which is reported mainly in the USA and Canada [35], Japan [36], South America [37] and in some European countries such as Austria [38]. The detected BVDV strain was further subtyped as BVDV-1b from one of the investigated dairy cattle farms.…”

Section: Discussionmentioning

confidence: 79%

Circulation of bovine viral diarrhea virus – 1 (BVDV-1) in dairy cattle and buffalo farms in Ismailia Province, Egypt

Soltan

Wilkes

Elsheery

et al. 2015

J Infect Dev Ctries

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Introduction: Bovine viral diarrhea (BVD) is one of the most economically significant diseases in the bovine industry causing losses due to diarrhea, reproductive disorders, immunosuppression and mortalities. The aim of our investigation was to detect and subtype BVDV from calves on two dairy cattle and two buffalo farms in Ismailia province, Egypt as an indicator of BVDV infection status in the province. Methodology: A total of 298 blood samples were collected and tested using an optimized one-step, real-time multiplex Taqman-based RT-PCR. All the positive samples by the multiplex real-time RT-PCR were tested using conventional RT-PCR to amplify multiple areas of the genome for further phylogenetic analysis and subtyping. Results: Thirty one (10.4%) of the tested samples were positive for BVDV-1. Only three samples, all from a single dairy cattle farm, had enough viral RNA to be amplified by RT-PCR. The PCR products were sequenced and phylogenetic analysis revealed detection of BVDV1b. The detected strain is closely related to worldwide BVDV-1b strains, making it difficult to trace its origin. Nucleotide and amino acid alignments of the E2 glycoprotein region of the detected strain with other BVDV-1b strains showed high divergence, with identity ranging from 81.3% to 93.6% and 85.3% to 93.6%, respectively. Conclusion: To our knowledge, this is the first report describing the circulation of BVDV-1b in Egyptian dairy cattle populations.

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Section: Discussionmentioning

confidence: 79%

Circulation of bovine viral diarrhea virus – 1 (BVDV-1) in dairy cattle and buffalo farms in Ismailia Province, Egypt

Soltan

Wilkes

Elsheery

et al. 2015

J Infect Dev Ctries

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“…A highly virulent BVDV genotype 2 was identified in severe outbreaks of acute haemorrhagic disease in Canada and the USA (Bolin & Ridpath, 1992;Pellerin et al, 1994). Analysis of BVDV field isolates indicated that BVDV-2 has also spread across Europe, for instance into Germany (Wolfmeyer et al, 1997), Italy (Pratelli et al, 2001), France (Vilcek et al, 2001), Belgium (Couvreur et al, 2002), Austria (Vilcek et al, 2003) and the UK (Wakeley et al, 2004), as well as South America (Canal et al, 1998) and Asia (Kim et al, 2006;Shimazaki et al, 1998). However, BVDV-2 is mainly a problem in North America, where type 2 BVDV is currently isolated nearly as frequently as BVDV-1 (Bolin & Ridpath, 1998;Evermann & Ridpath, 2002).…”

Section: Introductionmentioning

confidence: 99%

DNA prime–protein boost strategies protect cattle from bovine viral diarrhea virus type 2 challenge

Liang

Hurk

Landi

et al. 2008

Journal of General Virology

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At present, infections with bovine viral diarrhea virus (BVDV) type 2 occur nearly as frequently as those with BVDV type 1, so development of vaccines that protect cattle from both type 1 and type 2 BVDV has become critical. In this study, we compared various DNA prime-protein boost vaccination strategies to protect cattle from challenge with BVDV-2 using the major protective antigen of BVDV, glycoprotein E2. Calves were immunized with a plasmid encoding either type 1 E2 (E2.1) or type 2 E2 (E2.2) or with both plasmids (E2.1+E2.2). This was followed by a heterologous boost with E2.1, E2.2 or E2.1 and E2.2 protein formulated with Emulsigen and a CpG oligodeoxynucleotide. Subsequently, the calves were challenged with BVDV-2 strain 1373. All vaccinated calves developed both humoral and cell-mediated immune responses, including virus-neutralizing antibodies and IFN-c-secreting cells in the peripheral blood. Depletion studies showed that CD4+ T cells were responsible for IFN-c production. Furthermore, the calves vaccinated with either the E2.2 or the E2.1+E2.2 vaccines were very well protected from challenge with BVDV-2, having little leukopenia and showing no weight loss or temperature response. In addition, the animals vaccinated with the E2.1 vaccine were partially protected, so there was a certain level of cross-protection. These data demonstrate that a vaccination strategy consisting of priming with E2.2 or E2.1+E2.2 DNA and boosting with E2.2 or E2.1+E2.2 protein fully protects cattle from BVDV-2 challenge.

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“…In view of these facts Krametter-Froetscher et al (2007b) described that the main virus reservoir for pestivirus infection in sheep in Austria are cattle, but locally there exists also a virus reservoir of Border disease virus among sheep independent from cattle. Particularly in the mountainous regions, were communal alpine pasturing is a century old farming practice high seroprevalence levels in sheep were found in a survey carried out between and 2003(KrametterFroetscher et al, 2007b. The first cases of sheep persistently infected with Border disease virus in Austria were found in the alpine region of Vorarlberg in 2003 (Krametter-Froetscher et al, 2007a).…”

Section: Discussionmentioning

confidence: 99%

“…Therefore in the case described here the reservoir for the reintroduction of pestivirus infection in the cattle herd had been in all probability the sheep herd. Prior to the implementation of the BVDV eradication program, several studies about the genetic diversity of pestiviruses circulating in Austria only detected BVDV-strains in cattle (Vilcek et al, 2001(Vilcek et al, , 2003Kolesarova et al, 2004). With the eradication of BVDV and the resulting predominance of BDV, the pestivirus epidemiology in Austria possibly change, first evidence is given by Krametter-Froetscher et al (2008c) and Hornberg et al (2009) who detected cattle persistently infected with BDV.…”

Section: Discussionmentioning

confidence: 99%

BVDV control program in Austria - is a monitoring of the BDV status in sheep in Austria necessary?

Krametter-Froetscher¹,

Benetka²,

Rasser³

et al. 2009

Vet. Med. - Czech

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ABSTRACT:In cattle referred to the Clinic of Ruminants at the University of Veterinary Medicine Vienna by local veterinarians from Lower Austria, the number of animals positive for antibodies against pestiviruses decreased from 11.9% in 2004 to 7.4% in 2007. In other Austrian regions the seroprevalence of 17.6% in 2004 dropped to 12.2% in 2007. The seroprevalence rates were considerably higher in older animals than in younger indicating a marked decrease of new infections (8.2% in < 1.5 years old animals, 6.8% in 1.5-4.5 years, 19.8% in 4.5-7.5 years and 33.3% in > 7.5 years). These data nevertheless also demonstrate that new pestivirus infections occur, although at a lower rate. We report the case of a calf persistently infected with Border disease virus-3 (BDV-3) detected in a mixed cattle and sheep farm with the status "BVDV-free". Earlier investigations have shown that BDV-3 is endemic in Austrian sheep populations and seems to be a potential risk factor for the reintroduction of pestiviruses in BVDV free cattle herds. Serological findings among the investigated sheep population showed in four out of nine sheep samples considerably higher titres to the BDV strain Moredun than to the BVDV strain NADL. Seroconversion against pestiviruses was also detected in contact cattle and the mother of the persistently infected calf. Pestivirus specific RNA was neither detected in the blood samples collected from the patients of the Clinic for Ruminants nor in the sheep or cattle investigated on the farm described.

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Genetic diversity of recent bovine viral diarrhoea viruses from the southeast of Austria (Styria)

Cited by 31 publications

References 28 publications

Circulation of bovine viral diarrhea virus – 1 (BVDV-1) in dairy cattle and buffalo farms in Ismailia Province, Egypt

Circulation of bovine viral diarrhea virus – 1 (BVDV-1) in dairy cattle and buffalo farms in Ismailia Province, Egypt

DNA prime–protein boost strategies protect cattle from bovine viral diarrhea virus type 2 challenge

BVDV control program in Austria - is a monitoring of the BDV status in sheep in Austria necessary?

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