Genetic Diversity of Recka Sheep Breed in Albania Based on 15 Microsatellite Markers

Hoda, Anila; Vegara, Mensur; Bozgo, Vilson

doi:10.1080/13102818.2010.10817864

Cited by 3 publications

(4 citation statements)

References 12 publications

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“…Similar results have been reported by Stahlberger-Saibetkova et al [7] and Al-Atiyat et al [32]. However, lower estimates of genetic differentiation between sheep breeds were found by Ocampo et al [25], Bozzi et al [5], Zhong et al [30], Álvarez et al [33], Ben Sassi et al [34], Hoda et al [14] and Abdelkader et al [35], who reported Fst ranging between 0.05 and 0.3. The Fis value for the overall population (-0.02) is lower than the values reported in Turkish sheep breeds [36], Tunisian sheep breeds [37], Moroccan sheep breeds [38] and seven indigenous Bulgarian sheep breeds not included in this study [1].…”

Section: Population Genetic Diversitysupporting

confidence: 79%

“…Microsatellite markers (SSR, simple sequence repeats) have been extensively used for characterization of genetic diversity of indigenous breeds because of their high degree of polymorphism, co-dominant inheritance, locus specificity and extensive genome coverage. Recently, microsatellite markers were successfully applied for characterization of sheep (Ovis aries) genetic diversity, population structure and genetic differentiation, aiming development and application of genetic conservation strategies in different European countries like Austria, Hungary, Italy, Spain, Switzerland, [4][5][6][7][8][9] as well as in the countries from the Balkan Peninsula like Greece [10][11][12][13][14][15].…”

Section: Introductionmentioning

confidence: 99%

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Genetic diversity and structure of 2 indigenous sheep breeds (Kotel and Teteven) in Bulgaria using microsatellite markers

Mihailova

2021

Biotechnology & Biotechnological Equipment

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This study analyzed the genetic diversity and structure of two Bulgarian indigenous sheep (Ovis aries) breeds -Kotel and Teteven, each presented by 4 flocks, using 15 microsatellite markers. A total of 195 alleles were identified. Among the markers used, the most informative were INRA5 and OarFCB20 with PIC values of 0.87. Both breeds showed a high level of genetic diversity with average values of He and Ho, respectively 0.74 and 0.76. The observed high level of genetic diversity is combined with the absence of heterozygous deficiency reported as Fis values of 0.04 and 0.07, respectively for Kotel and Teteven breeds. Low level of genetic differentiation between the two breeds was observed, as evidenced by the low value of Fst of 0.09, and analysis of variance (ANOVA) data showing that only 9% of the total variation is due to variation between breeds, whereas 91% is due to variation within breeds. Furthermore, we revealed a breeding practice that comprises an exchange of animals between the breeds which reflects in the clustering obtained by STRUCTURE analysis. According to it, Kotel breed is a more homogenous population and forms cluster 1, whereas Teteven breed is more heterogenous and consists of two subpopulations that form two distinct clusters. The results obtained in the present study confirm the high potential of microsatellite markers in studies related to both breed affiliation and purity and can serve as a basis for the implementation of future management and conservation programs of these indigenous breeds.

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Section: Population Genetic Diversitysupporting

confidence: 79%

Section: Introductionmentioning

confidence: 99%

Genetic diversity and structure of 2 indigenous sheep breeds (Kotel and Teteven) in Bulgaria using microsatellite markers

Mihailova

2021

Biotechnology & Biotechnological Equipment

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“…Additional criteria used for selection of core SSR markers include: (1) PID < 0.198 (average of the 33 markers), such that a combination of a few markers provided enough discriminant power; (2) high degree of polymorphism, with a PIC > 0.5 (Hoda et al 2010 ; Pan et al 2010 ); and (3) finally, the number of alleles ≤5, and the number of genotypes ≤10. Primers providing higher numbers of alleles and of genotypes were not chosen because they were not deemed to be easily manageable.…”

Section: Methodsmentioning

confidence: 99%

An efficient identification strategy of clonal tea cultivars using long-core motif SSR markers

et al. 2016

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Microsatellites, or simple sequence repeats (SSRs), especially those with long-core motifs (tri-, tetra-, penta-, and hexa-nucleotide) represent an excellent tool for DNA fingerprinting. SSRs with long-core motifs are preferred since neighbor alleles are more easily separated and identified from each other, which render the interpretation of electropherograms and the true alleles more reliable. In the present work, with the purpose of characterizing a set of core SSR markers with long-core motifs for well fingerprinting clonal cultivars of tea (Camellia sinensis), we analyzed 66 elite clonal tea cultivars in China with 33 initially-chosen long-core motif SSR markers covering all the 15 linkage groups of tea plant genome. A set of 6 SSR markers were conclusively selected as core SSR markers after further selection. The polymorphic information content (PIC) of the core SSR markers was >0.5, with ≤5 alleles in each marker containing 10 or fewer genotypes. Phylogenetic analysis revealed that the core SSR markers were not strongly correlated with the trait ‘cultivar processing-property’. The combined probability of identity (PID) between two random cultivars for the whole set of 6 SSR markers was estimated to be 2.22 × 10−5, which was quite low, confirmed the usefulness of the proposed SSR markers for fingerprinting analyses in Camellia sinensis. Moreover, for the sake of quickly discriminating the clonal tea cultivars, a cultivar identification diagram (CID) was subsequently established using these core markers, which fully reflected the identification process and provided the immediate information about which SSR markers were needed to identify a cultivar chosen among the tested ones. The results suggested that long-core motif SSR markers used in the investigation contributed to the accurate and efficient identification of the clonal tea cultivars and enabled the protection of intellectual property.

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“…The Turcana breed is one of the most commonly raised sheep breeds in Romania (Coroian et al., 2009), preferred for its resilience against harsh geography and weather conditions, as well as natural factors such as parasites (Gavojdian et al., 2011). In extensive and semi‐extensive farming systems, utilizing pasture and grassland areas efficiently, Bardoka boasts triple‐purpose characteristics—milk, meat and wool—holding significant socio‐economic importance for the local farmer community (Hoda & Hajno, 2021). The Polish Mountain Sheep, raised in Poland, is said to be the only sheep breed used for milk production in the production of dairy products such as cheese and yogurt; thus, it plays an important role in cheese production in Poland (Radzik‐Rant et al., 2011).…”

Section: Introductionmentioning

confidence: 99%

Geometric morphometric evaluation of mandibles of four sheep breeds: Bardoka, İvesi, Polish Mountain sheep and Turcana

Özkan,

Boz Doğan,

Duro

et al. 2024

Anat Histol Embryol

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The enduring relationship between humans and domestic sheep has evolved over millennia, showcasing diverse uses such as meat, milk, wool, leather and fur, shaped by geographical, historical, cultural and social factors. The sheep breeds discussed include the Ivesi from Southeastern Anatolia, known for its varied animal products; the resilient Turcana breed of Romania; Kosovo's Bardoka, valued for its triple‐purpose characteristics; and Poland's Polish Mountain Sheep, uniquely utilized for milk production in cheese making. Sheep, with their enduring relationship with humans and significant economic importance, have attracted scientific interest in morphometric studies of their mandibles, yielding valuable data applicable across various fields including basic anatomy, veterinary clinical anatomy, zooarchaeology and veterinary forensic medicine. Traditional morphometric studies rely on statistical methods to compare length, depth and angular ratios between anatomical formations, often highlighting differences between specific points but not fully revealing shape variations between distinct groups. Geometric morphometric analysis has emerged as a preferred method in recent years, enabling shape analyses using coordinate data from various imaging techniques, facilitating a comprehensive examination of mandibular morphometrics among sheep breeds across different countries. This study involved four sheep breeds from different countries, namely İvesi from Turkey, Bardoka from Kosovo, Polish Mountain Sheep from Poland and Turcana from Romania, with a total of 70 mandibles sourced from various veterinary faculties. Mandibular photographs were meticulously captured, focusing on the right side of mandible pairs and placing landmarks and semi‐landmarks along the entire edge, enabling geometric morphometric analysis using tpsUtil, tpsDig2 and MorphoJ software. The analysis included principal component analysis, canonical variate analysis and discriminant function analysis for pairwise comparisons, facilitating a comprehensive examination of mandibular shape variations among the different sheep breeds. Using geometric morphometric methods, this study analysed mandibles from four distinct sheep breeds sourced from different countries, revealing notable variations in regions such as the ramus mandibula, angulus mandibula and incisive areas, attributed to genetic, geographical and dietary influences, highlighting the importance of continued research to better comprehend these shape differences.

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Genetic Diversity of Recka Sheep Breed in Albania Based on 15 Microsatellite Markers

Cited by 3 publications

References 12 publications

Genetic diversity and structure of 2 indigenous sheep breeds (Kotel and Teteven) in Bulgaria using microsatellite markers

Genetic diversity and structure of 2 indigenous sheep breeds (Kotel and Teteven) in Bulgaria using microsatellite markers

An efficient identification strategy of clonal tea cultivars using long-core motif SSR markers

Geometric morphometric evaluation of mandibles of four sheep breeds: Bardoka, İvesi, Polish Mountain sheep and Turcana

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