Genetic diversity of red and black upland rice accessions from East Nusa Tenggara, Indonesia as revealed by agro-morphological characters

Abstract

Abstract. Firawati I, Murwantoko, Setyobudi E. 2017. Morphological and molecular characterization of hairtail (Trichiurus spp.) from Indian Ocean, southern coast of East Java,. The objective of this study was to identify species of hairtails (Trichiurus spp.) which caught from the southern coast of East Java, based on morphology and molecular characters. Three hundred and forty-nine fish samples were collected from two fishing port i.e. Muncar and Prigi. Twenty-two morphometric and four meristic characters were measured from each sample. The result showed that the hairtail samples consisted of two species, T. lepturus and T. brevis based on morphology characters. Based on Principal Component Analysis (PCA) and Discriminant Function Analysis (DFA), these species can be distinguished by the head and caudal character, which the length of an upper jaw and caudal peduncle characters were the main distinguish. Analysis of fragment (~600 bp) of the mitochondrial DNA encoding 16S rRNA gene showed that T. lepturus samples were 100% genetically identical with T. lepturus while T. brevis samples were 100% identical with T. brevis. Phylogenetic analysis showed that T. lepturus and T. brevis were distinct species while T. lepturus both which caught from Prigi and Muncar were closely related species. The molecular identification can be used to verify the result of morphological identification.

Morphological and molecular identification of hairtail (Trichiurus spp.) caught in Pangandaran Waters

“…Based on the principal component analysis (PCA), distinguishing characters to differentiate Pangandaran's hairtail were caudal peduncle and parts around the body. The previous study reported that the characteristics to differentiate T. lepturus from T. brevis in Prigi and Muncar were located in the head and tail [4]. Moreover, the discriminant analysis showed that meristic characteristics of upper jaw length and caudal peduncle length can also be used.…”

“…Based on morphological and molecular analysis, there are only two species i.e. T. lepturus and T. brevis were found at the Indian Ocean Southern coast of East Java [4]. T. lepturus has a very broad geographical distribution, covers tropical and temperate waters throughout the world.…”

Variability, heritability, and performance of 28 West Sumatran upland rice cultivars, Indonesia

“…Phapumma et al (2020) reported that plants with a tall appearance are not favorable traits for grain yield. Plants with a semi-dwarf performance are more desirable than tall ones because semi-dwarf plants are stronger in facing environmental factors such as wind and rain (Mau et al 2017;Hermanasari et al 2021). Hence semi-dwarf plant height becomes an essential trait for the breeder in the rice improvement program.…”

“…Comprehensive knowledge of genetic availability and diversity is needed for crop improvement and the future development of new high-yielding varieties (Malek et al 2014;Adie and Krisnawati 2017;Weerakoon and Somarate 2021). The study of genetic diversity in local upland rice cultivars based on agro-morphological traits can provide reliable information for selection and further work on producing new superior varieties (Mau et al 2017). The higher variability of, the traits the immense opportunity to obtain the desirable traits and candidate as a new potential cultivar.…”

Genetic diversity of selected pigmented traditional rice (Oryza sativa L.) varieties from Mindanao, Philippines using agromorphological traits and simple sequence repeats markers

“…Thousands of SSR markers were developed for rice research studies (Huggins et al 2018). It has been used for molecular characterization, population structure, genotypic identification, and genetic variability estimations (Mau et al 2017;Islam et al 2018). Furthermore, it has also proven useful for linkage maps construction, gene mapping, MAS (Gonzaga et al 2015) and diversity studies (Rashimi et al 2017).…”