Genetic diversity of respiratory syncytial virus isolated during an epidemic period from children of northeastern Brazil

Abstract

Outbreaks of human respiratory syncytial virus (HRSV) are the leading cause of serious acute lower respiratory viral disease in many countries in different continents. Data on clinical and epidemiological aspects of HRSV infections in this country have been reported, but there is lack of data regarding the molecular epidemiology of this virus in Salvador. The genetic variability of HRSV isolated during an outbreak in Salvador, Brazil (1999) has been analysed. Partial sequences of the G protein gene of 13 isolates from antigenic group A and 4 isolates from antigenic group B of HRSV were determined. Nucleotide sequences of C-terminal G gene were compared to sequences of HRSV isolates from countries of South America and from the rest of the world available at the GenBank. Brazilian group A and B isolates were clustered into previously characterised genotypes: GA5, GA2, GA7, and GB3, SAB3, respectively. This is the first study of GA7 and SAB3 genotypes circulation in South American countries. It is interesting to point out that viruses isolated in Salvador appear to be closer related with those from Montevideo-Uruguay and Buenos Aires, Argentina strains, suggesting circulation of similar strains among different South American countries in different seasons. Moreover, viruses closely related genetically circulated in the same year in Salvador and distant places such as Mozambique, supporting the previous suggestion on the complexity of HRSV strain circulation patterns, and the high capability of HRSV spreading world-wide.

Molecular epidemiology of human respiratory syncytial virus in Uruguay: 1985-2001 - A review

“…2), one of them with very few strains, while the other has several sub-branches, in agreement with other reports from several countries (García et al 1994, Peret et al 1998, Coggins et al 1998, Cane 2001, Venter et al 2001, Moura et al 2004). The branch with very few sub-branches clustered isolates with bootstrap value of 100% within GA1 genotype.…”

“…Studies on molecular epidemiology of HRSV have focused on this protein, showing several evolutionary lineages among isolates of both groups (Cane et al 1991, Garcia et al 1994, Peret et al 1998, Martinez et al 1999, Venter et al 2001, Moura et al 2004). The domain located near the C-terminal region of the G protein reflects the entire G gene variability; it has therefore been used in phylogenetic analyses for molecular epidemiology studies (Peret et al 1998, Martínez et al 1999.…”

Molecular epidemiology and genetic variability of respiratory syncytial virus (RSV) in Stockholm, 2002–2003

“…In all 12 patients, sequences from the sequential sampling were identical and no genetic variation in the N-terminal region of the G gene could be demonstrated during the phase of illness. International data from molecular epidemiological studies of RSV are derived from long-term studies with 100 or more isolates [Peret et al, 1998;Choi and Lee, 2000;Seki et al, 2001;Venter et al, 2001;Scott et al, 2004;Kuroiwa et al, 2005;Sato et al, 2005;Viegas and Mistchenko, 2005], from long-term studies with a more limited number if isolates [Cane et al, 1994;Garcia et al, 1994;Zambon et al, 2001;Venter et al, 2002;Frabasile et al, 2003;Rafiefard et al, 2004;Galiano et al, 2005;Parveen et al, 2006], and also from shortterm studies like this one from Stockholm [Peret et al, 2000;Moura et al, 2004]. This study provides data from a complete season.…”

“…The epidemiology and the genetic diversity of RSV have been studied in many parts of the world, among them, the Gambia [Cane et al, 1999], Korea [Choi and Lee, 2000], Uruguay and Argentina [Frabasile et al, 2003], Argentina [Galiano et al, 2005], Japan [Kuroiwa et al, 2005;Sato et al, 2005], South Africa [Venter et al, 2001;Venter et al, 2002;Madhi et al, 2003], Brazil [Moura et al, 2004], India [Parveen et al, 2006], USA [Peret et al, 1998;Peret et al, 2000], Kenya [Scott et al, 2004], Great Britain [Zambon et al, 2001], and Belgium [Zlateva et al, 2005]. At least three studies from Europe have been published during the past decade.…”

Variabilidade genética da proteína SH (Small hydrophobic protein) do vírus sincicial respiratório humano isolado de crianças na cidade de São Paulo.

“…Cada epidemia dura, aproximadamente, 5 meses sendo que a grande maioria dos casos ocorre durante os meses de pico, normalmente no meio do surto. (Collins, et al, 2001 (Moura, et al, 2004;Girard, et al, 2005; No Brasil, a sazonalidade apresentada pelo VSRH é bastante marcante e similar em diferentes localidades do país como em São Paulo, Fortaleza, Salvador, Uberlândia, nos quais os surtos começam em janeiro ou fevereiro, estendendo-se predominantemente, pelo outono-inverno com pico em maio e junho (Viera, et al, 2001;Moura, et al, 2006;Thomazelli, et al, 2007).…”