Genetic Diversity of Rotaviruses Circulating in Pediatric Patients and Domestic Animals in Thailand

Jampanil, Nutthawadee; Maneekarn, Niwat; Khamrin, Pattara

doi:10.3390/tropicalmed8070347

Cited by 4 publications

(2 citation statements)

References 67 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…VP2 connected to VP1 (RNA-dependent RNA polymerase) and VP3 (viral capping enzyme) make up the internal core. The complete viral genome, which consists of 11 double-stranded RNA (dsRNA) segments, is enclosed within the internal core layer (Jampanil et al, 2023). The rotavirus genome codes for six nonstructural proteins (NSP1-NSP6) and six viral structural proteins (VP1-VP4, VP6, and VP7) (Desselberger, 2014).…”

Section: Rva Molecular Biologymentioning

confidence: 99%

Rotavirus in developing countries: molecular diversity, epidemiological insights, and strategies for effective vaccination

Sadiq,

Khan

2024

Front. Microbiol.

View full text Add to dashboard Cite

Rotavirus (RV) causes the loss of numerous children’s lives worldwide each year, and this burden is particularly heavy in low- and lower-middle-income countries where access to healthcare is limited. RV epidemiology exhibits a diverse range of genotypes, which can vary in prevalence and impact across different regions. The human genotypes that are most commonly recognized are G1P[8], G2P[4], G3P[8], G4P[8], G8P[8], G9P[8], and G12P[8]. The diversity of rotavirus genotypes presents a challenge in understanding its global distribution and developing effective vaccines. Oral, live-attenuated rotavirus vaccines have undergone evaluation in various contexts, encompassing both low-income and high-income populations, demonstrating their safety and effectiveness. Rotavirus vaccines have been introduced and implemented in over 120 countries, offering an opportunity to assess their effectiveness in diverse settings. However, these vaccines were less effective in areas with more rotavirus-related deaths and lower economic status compared to wealthier regions with fewer rotavirus-related deaths. Despite their lower efficacy, rotavirus vaccines significantly decrease the occurrence of diarrheal diseases and related mortality. They also prove to be cost-effective in regions with a high burden of such diseases. Regularly evaluating the impact, influence, and cost-effectiveness of rotavirus vaccines, especially the newly approved ones for worldwide use, is essential for deciding if these vaccines should be introduced in countries. This is especially important in places with limited resources to determine if a switch to a different vaccine is necessary. Future research in rotavirus epidemiology should focus on a comprehensive understanding of genotype diversity and its implications for vaccine effectiveness. It is crucial to monitor shifts in genotype prevalence and their association with disease severity, especially in high-risk populations. Policymakers should invest in robust surveillance systems to monitor rotavirus genotypes. This data can guide vaccine development and public health interventions. International collaboration and data sharing are vital to understand genotype diversity on a global scale and facilitate the development of more effective vaccines.

show abstract

Section: Rva Molecular Biologymentioning

confidence: 99%

Rotavirus in developing countries: molecular diversity, epidemiological insights, and strategies for effective vaccination

Sadiq,

Khan

2024

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…The most frequent genotypes of CRV are G3P [3], and G3P [9] [4]. CPV is a non-enveloped, single-stranded negative-sense DNA virus with a genome of approximately 5.2 kb and it belongs to the Protoparvovirus genus in the Parvoviridae family [5].…”

Section: Introductionmentioning

confidence: 99%

A Quadruplex Reverse Transcription Quantitative Polymerase Chain Reaction for Detecting Canine Coronavirus, Canine Rotavirus, Canine Parvovirus, and Canine Distemper Virus

Shi,

Long,

Shi

et al. 2024

Microbiology Research

View full text Add to dashboard Cite

Background: Canine coronavirus (CCoV), canine rotavirus (CRV), canine parvovirus (CPV), and canine distemper virus (CDV) cause gastroenteritis in dogs, and co-infections of these pathogens are common in China. In particular, CCoV and CRV are confirmed to have important zoonotic potential and cause public health issues. It is difficult to diagnose these diseases based only on clinical manifestations and pathological damage. Methods: In this study, four pairs of specific primers and probes targeting the CCoV M, CRV VP7, CPV VP2, and CDV N genes were designed. The reaction conditions, including the primer and probe concentrations, annealing temperatures, and reaction cycles, were optimized for the development of a quadruplex RT-qPCR for the detection of CCoV, CRV, CPV, and CDV. The assay was used to test 1028 clinical samples to validate its application. Results: A quadruplex RT-qPCR was successfully established for the differential detection of CCoV, CRV, CPV, and CDV, with good specificity, high sensitivity, and excellent repeatability. The assay could specifically detect CCoV, CRV, CPV, and CDV without cross-reactivity with the other canine viruses tested. It showed high sensitivity with limits of detection (LOD) of 1.1 × 102 copies/reaction for all four plasmid constructs. It showed excellent repeatability, with 0.05–0.90% intra-assay variation and 0.02–0.94% inter-assay variation. The 1028 clinical samples were tested using the quadruplex RT-qPCR and a reported reference RT-qPCR. The positivity rates of CCoV, CRV, CPV, and CDV were 9.53%, 0.97%, 25.68%, and 5.06% using the developed assay, and 9.05%, 0.88%, 25.68%, and 4.86% using the reference assay, with agreements higher than 99.32%. Conclusion: The results indicated that a rapid and accurate quadruplex RT-qPCR was developed for the detection and differentiation of CCoV, CRV, CPV, and CDV.

show abstract

Evaluation of a novel triplex immunochromatographic test for rapid simultaneous detection of norovirus, rotavirus, and adenovirus on a single strip test

Ushijima,

Pham,

Hoque

et al. 2024

Journal of Infection and Public Health

View full text Add to dashboard Cite

Genetic Diversity of Rotaviruses Circulating in Pediatric Patients and Domestic Animals in Thailand

Cited by 4 publications

References 67 publications

Rotavirus in developing countries: molecular diversity, epidemiological insights, and strategies for effective vaccination

Rotavirus in developing countries: molecular diversity, epidemiological insights, and strategies for effective vaccination

A Quadruplex Reverse Transcription Quantitative Polymerase Chain Reaction for Detecting Canine Coronavirus, Canine Rotavirus, Canine Parvovirus, and Canine Distemper Virus

Evaluation of a novel triplex immunochromatographic test for rapid simultaneous detection of norovirus, rotavirus, and adenovirus on a single strip test

Contact Info

Product

Resources

About