Genetic Diversity of some Daffodil (Narcissus L. spp.) Genotypes in Turkey by Using SRAP Markers

Zeybekoǧlu, Emrah; Asciogul, Tansal Kaygisiz; İlbi, Hülya; Özzambak, Mustafa Ercan

doi:10.15835/nbha47411664

Cited by 2 publications

(1 citation statement)

References 11 publications

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“…ISSR markers has been used well for genetic diversity of cuscuta (Tajdoost et al, 2012), Persian violet (Asadi et al, 2016), narcissus (Jiménez et al, 2009), Solanum nigrum L. (Suganthi et al, 2018). Also, genetic diversity of different Turkish narcissus has been studied using SRAP markers (Zeybekoğlu et al, 2019). A study using markers (ISSR) investigated the genetic diversity of 31 narcissus species collected from 16 regions of Iran.…”

Section: Introductionmentioning

confidence: 99%

Genetic variation and cluster analysis of Narcissus tazetta L. genotypes using RAPD and ISSR markers

ZABET¹,

ESMAEILZAEI²,

IZANLOO³

et al. 2023

Acta Agric. Slov.

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To evaluate the genetic diversity of Iranian Narcissus genotypes (e.g., Shomal, Shastpar, Shahla, Yasuj, Shiraz-1, Shiraz-2, Kuchak-e-Atri, Dutch, Khosf-1, Khosf-2, Birjand, and Tabas), different RAPD and ISSR primers were examined at the Plant Breeding Laboratory of the Faculty of Agriculture, University of Birjand, Iran. In sum, RAPD and ISSR primers produced 189 and 80 high-resolution bands. The average values of PIC, Ht, Hs, Nm, DST, FDT, NA, Ne, H, and I indices were 0.287, 0.369, 0.089, 0.486, 0.279, 0.760, 1.952, 1.459, 0.282, 0.437 for RAPD markers and equal to 0.297, 0.380, 0.099, 0.524, 0.278, 0.732, 1.978, 1.495, 0.303, 0.467 for ISSR markers, respectively. The analysis of molecular variance based on the RAPD and ISSR markers showed 23 and 13 % variations for intra-populations and 77 % and 87 % changes for inter-population variabilities, respectively. Cluster analysis based on the RAPD and ISSR markers grouped genotypes into four clusters. Based on RAPD and ISSR markers, the PCoA analysis also showed that the first three components justified equal to 96.9 % and 97.9 % of the total variance, respectively, indicating the dispersion of the primers used. In general, it was concluded that the genetic diversity of narcissus species could be employed for breeding programs.

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Section: Introductionmentioning

confidence: 99%

Genetic variation and cluster analysis of Narcissus tazetta L. genotypes using RAPD and ISSR markers

ZABET¹,

ESMAEILZAEI²,

IZANLOO³

et al. 2023

Acta Agric. Slov.

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Variability of Agave amica in India Using SRAP Markers and Multivariate Analysis of Morphological Traits

PR,

AY,

et al. 2024

Preprint

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The genus Agave, indigenous to Mexico, comprises approximately 15 species, 3 varieties, and a few commercially cultivated cultivars. Despite its ornamental value and global economic importance, the development of new cultivars has been limited, with only two primary varieties and around 20 single and double-flower cultivars currently in cultivation, restricting the genetic diversity available to breeders. This study investigates the genetic and morphological diversity among thirteen tuberose (Agave amica) cultivars using Sequence-Related Amplified Polymorphism (SRAP) markers and morphological assessments. SRAP analysis generated 63 scorable bands, with 54 polymorphic and 9 monomorphic bands, resulting in about 81% polymorphism. Cluster analysis via the NTSYS-pc program grouped the cultivars into two major clusters, with genetic similarity coefficients ranging from 0.51 to 0.89, indicating significant genetic variation. Within Cluster I, Hyderabad Single and Arka Prajwal showed 85.93% similarity, while Arka Sugandhi shared 84.37% similarity with both. Arka Nirantara formed a distinct subgroup, showing 84.37% similarity with Arka Prajwal and 78.12% with Arka Sugandhi. In Cluster II, Bidhan Ujjwal and Arka Vaibhav exhibited high relatedness with 89.06% similarity. Sikkim Selection and Mexican Single formed a subgroup within this cluster. SRAP primers showed a resolving power ranging from 6 to 25, with an average of 3.85 polymorphic bands per primer pair and a PIC value of 0.528, demonstrating their effectiveness in distinguishing among cultivars. Morphological evaluation of 11 traits, including plant height, leaf dimensions, flowering period, and spike characteristics, revealed significant variability. Multifactorial Analysis (MFA) and Agglomerative Hierarchical Clustering (AHC) identified key traits contributing to this diversity. The integration of molecular and morphological data offers a thorough understanding of the genetic and phenotypic diversity in tuberose, crucial for breeding, conservation, and the development of improved cultivars, as well as the effective conservation of its germplasm.

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Genetic Diversity of some Daffodil (Narcissus L. spp.) Genotypes in Turkey by Using SRAP Markers

Cited by 2 publications

References 11 publications

Genetic variation and cluster analysis of Narcissus tazetta L. genotypes using RAPD and ISSR markers

Genetic variation and cluster analysis of Narcissus tazetta L. genotypes using RAPD and ISSR markers

Variability of Agave amica in India Using SRAP Markers and Multivariate Analysis of Morphological Traits

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