Genetic diversity of some species in the genus <i>Calanthe</i> analyzed by RAPD markers

Punjansing, Tasanai; Chaichanachap, N.; Sanitchon, Jirawat; Pinta, W.; Nakkuntod, Maliwan

doi:10.17660/actahortic.2017.1167.46

Cited by 1 publication

(1 citation statement)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…DNA products were amplified using RAPD primers followed by the previous protocol [45] and 20 primer pairs were selected in this study, though these primers only relied on previous detection of 3 orchid species via the RAPD technique. Molecular markers for identifying Phaius species have been previously studied using the SRAP marker technique.…”

Section: Discussionmentioning

confidence: 99%

Production and Molecular Identification of Interspecific Hybrids between Phaius mishmensis (Lindl. and Paxton) Rchb. f. and Phaius tankervilliae (Banks) Blume

et al. 2021

View full text Add to dashboard Cite

This study aimed at assessing the hybridization feasibility and evaluating genetic fidelity of the hybrid seedlings originated from Phaius mishmensis (Lindl. and Paxton) Rchb. f. and P. tankervilliae (Banks) Blume. Intra- and interspecific hybridization between Phaius mishmensis (Lindl. and Paxton) Rchb. f. and P. tankervilliae (Banks) Blume were examined to establish the primary hybrid, observe their cross ability and identify the F1 hybrids using sequence-characterized amplified region (SCAR) markers. Self-incompatibility and cross ability of P. mishmensis and P. tankervilliae were tested before starting the breeding program. Results showed that they were self-compatible orchids. The interspecific hybridization between P. mishmensis and P. tankervilliae was achieved with the highest pod setting (80%), seed germination percentage (94.8%) and the rate of protocorm development into mature seedlings (stage 6) (10.6%), but the smallest size of embryo with width 46.5 μm, length 67.3 μm was also observed when P. mishmensis was taken as the female parent. A comparative study on leaf morphology and anatomy of plantlets regenerated from intra- and interspecific hybrids of P. mishmensis and P. tankervilliae showed a transitional character to the parental species. Herein, the presence of interspecific hybrids between P. mishmensis and P. tankervilliae, as well as their reciprocal cross, was verified using Pmis524 SCAR markers developed by the decamer primer.

show abstract

Section: Discussionmentioning

confidence: 99%