Genetic diversity of SSR and ISSR markers in wild populations of Brachypodium distachyon and its close relatives B. stacei and B. hybridum (Poaceae)

Hammami, Rifka; Jouve, N.; Soler, Consuelo; Frieiro, Eva; González, Juan M.

doi:10.1007/s00606-014-1021-0

Cited by 18 publications

(14 citation statements)

References 46 publications

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“…For instance, genotype Bs115 is more related with three genotypes of B. distachyon than the other three genotypes of B. stacei, Moreover, Bd160 and Bh486, appear clearly separated on the dendrogram, probably due to these genotypes have, respectively, the shortest and the largest radicular system of the genotypes of B. distachyon and B. hybridum analysed. These results are similar to those obtained by other authors using biochemical and molecular markers to differentiate between the members of a large collection of genotypes belonging to the present three species of Brachypodium (including those studied in the present work) (Hammami et al 2011(Hammami et al , 2014. The overlap observed in the grouping of the genotypes of the three species can be explained by the existing evolutionary proximity among them, being B. hybridum an allotetraploid of the other two species.…”

Section: Discussionsupporting

confidence: 92%

A comparative study of root system architecture in seedlings ofBrachypodium spp.using three plant growth supports

González

Friero

Selfa

et al. 2016

Cereal Research Communications

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This work reports a rapid procedure of comparing root development among different genotypes of Brachypodium spp., using three plant growth supports: gel chamber, 'Termita' chamber and Whatman paper. Eight variables of the root system architecture (RSA) (number of seminal roots, number of lateral roots, total length of the roots, length of the primary root, mean diameter of the roots, mean diameter of the primary root, total surface area and total volume of the roots) were studied in seedling of four genotypes each of Brachypodium distachyon, B. stacei and B. hybridum. Correlations between pairs of growth supports in terms of the eight variables examined were highly significant. In all three supports, B. stacei showed the greatest root system development while B. distachyon showed the least; B. hybridum, an allotetraploid species derived from hybridization between B. distachyon and B. stacei, showed intermediate development. ANOVA and LSD tests showed that significant differences exist between the supports, species and genotypes with respect to all the variables analysed. A cluster analysis was conducted to determine if the RSA traits could be used to differentiate the species and genotypes of Brachypodium. This analysis allowed differentiated between the three species and twelve genotypes of Brachypodium spp., although a certain overlap between species was observed. The Whatman paper support was the easiest to use, and is recommended for the characterization of large collections of genotypes.

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Section: Discussionsupporting

confidence: 92%

A comparative study of root system architecture in seedlings ofBrachypodium spp.using three plant growth supports

González

Friero

Selfa

et al. 2016

Cereal Research Communications

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“…[10,16–18]. ISSR is cheap and effective genotyping technique that have been used for determining genetic diversity [19,20]. ISSRs detect variations of DNA fragments located between two adjacent, identical and opposite orientated microsatellites [21].…”

Section: Introductionmentioning

confidence: 99%

Promoting the Use of Common Oat Genetic Resources through Diversity Analysis and Core Collection Construction

et al. 2016

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The assessment of diversity and population structure and construction of a core collection is beneficial for the efficient use and management of germplasm. A unique collection of common oat landraces, cultivated in the temperate climate of central Europe until the end of the twentieth century, is preserved in the Polish gene bank. It consists of 91 accessions that have never been used in breeding programs. In order to optimise the use of this genetic resource, we aimed to: (1) determine genetic and agro-morphological diversity, (2) identify internal genetic variation of the tested accessions, (3) form a core collection and (4) recognise the accessions useful for breeding programs or re-release for cultivation. The collection was screened using ISSR markers (1520 loci) and eight agro-morphological traits. Uniquely, we performed molecular studies based on 24 individuals of every accession instead of bulk samples. Therefore, assessment of the degree of diversity within each population and the identification of overlapping gene pools were possible. The observed internal diversity (Nei unbiased coefficient) was in the range of 0.17–0.31. Based on combined genetic and agro-morphological data, we established the core collection composed of 21 landraces. Due to valuable compositions of important traits, some accessions were also identified as useful for breeding programs. The population structure and principal coordinate analysis revealed two major clusters. Based on the previous results, the accessions classified within the smaller one were identified as obsolete varieties instead of landraces. Our results show that the oat landraces are, in general, resistant to local races of diseases, well adapted to local conditions and, in some cases, yielding at the level of modern varieties. Therefore, in situ conservation of the landraces in the near future may be satisfactory for both farmers and researchers in terms of the genetic resources preservation.

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“…This result appears to be contrary to the general idea that polyploids are better adapted to the most adverse environmental conditions and therefore grow at higher altitudes or in drier conditions [43]. However, these differences in seedling development may reflect adaptation to different ecological requirements as B. distachyon grows in higher altitudes (Table 1) and drier areas, while B. hybridum grows in lower and warmer areas and B. stacei occupies intermediate areas [33,44].…”

Section: Drought Influence In Rsa Traits and Shoot Dry Weight In Bracmentioning

confidence: 68%

“…Eleven sets of primers from http://www.brachypodium. pw.usda.gov/SSR/ series were used to amplify SSR regions as described in Hammami et al [33].…”

Section: Ssr Analysismentioning

confidence: 99%

“…B. distachyon was considered to be formed by three cytotypes with 10, 20, and 30 chromosomes. However, cytogenetic [28,29] and biochemical and molecular studies [29][30][31][32][33] have shown that there are three species: Two diploid B. distachyon (2n = 10) and B. stacei (2I = 20) and one tetraploid B. hybridum (2n = 30), derived from the hybridization and subsequent chromosomal duplication of distachyon and stacei [27,34,35]. These three species are proposed as a model polyploid speciation of grasses [27,[34][35][36] and may also contribute to the breeding of cultivated polyploid cereals.…”

Section: Introductionmentioning

confidence: 99%

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Molecular Genetic Analysis of Drought Stress Response Traits in Brachypodium spp.

et al. 2020

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The root is the organ responsible for the uptake of water and therefore has a very important role in drought tolerance. The aims of the present work were to characterize nine traits of the root system architecture (RSA) and the shoot dry weight (W) of twelve genotypes of Brachypodium spp. under water stress and to establish the relationship between RSA phenotyping traits and SSRs. Two culture media, one standard (SM) and one (PEG) to induced water stress have been used. In SM medium, B. stacei had the highest values of W and all the RSA traits, except the mean diameter of the seminal roots, followed by B. hybridum and B. distachyon. In the PEG medium, root length increased in B. distachyon, decreased in B. hybridum and remained the same in B. stacei. A two-way hierarchical cluster analysis from 117 polymorphic SSRs and the traits of the RSA of the Brachypodium spp. genotypes, was performed. Brachypodium genotypes were separated into three groups corresponding to each species. In the second way of the hierarchical clustering association were observed between five RSA variables and SSR markers, which could be useful in the search for genes or QTLs related to RSA characters.

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Genetic diversity of SSR and ISSR markers in wild populations of Brachypodium distachyon and its close relatives B. stacei and B. hybridum (Poaceae)

Cited by 18 publications

References 46 publications

A comparative study of root system architecture in seedlings ofBrachypodium spp.using three plant growth supports

A comparative study of root system architecture in seedlings ofBrachypodium spp.using three plant growth supports

Promoting the Use of Common Oat Genetic Resources through Diversity Analysis and Core Collection Construction

Molecular Genetic Analysis of Drought Stress Response Traits in Brachypodium spp.

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