Genetic diversity of the bacterial wilt pathogen Ralstonia solanacearum using a RAPD marker

Nishat, Sayeda; Hamim, Islam; Khalil, M.I.; Ali, Ayub; Hossain, M. A.; Meah, M. B.; Islam, Rashidul

doi:10.1016/j.crvi.2015.06.009

Cited by 24 publications

(16 citation statements)

References 34 publications

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“…shows the difference between avirulent colonies that look dark red from the fluidal virulent that are white with pink center [25,29]. In the present study, the colonies were fluidal whitish with a pink center, indicating virulent species of R. solanacearum.…”

Section: Morphological Profiling Of Ralstonia Solanacearummentioning

confidence: 54%

Isolation and Characterisation of Ralstonia Solanacearum Strains of Tomato Wilt Disease from Maseno, Kenya

Khasabulli

Musyimi

Miruka

et al. 2017

Journal of Asian Scientific Research

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Article HistoryRalstonia solanacearum (E.F. Smith) Yabuuchi, is the cause of bacterial wilt of tomato and one of the most severe pathogens of solanaceous crops with a very wide host range. There is little information on R. solanacearum strains in Maseno region in Kenya despite the fact that bacterial wilt of tomato is of economic importance. The objective of this study was to isolate the strains of R. solanacearum of tomato and characterize morphologically and biochemically Ralstonia solanacearum races and biovars from infected tomato plants in Maseno region. This study was conducted at Maseno University in the Department of Botany at Microbiology Laboratory. Ten diseased tomato plants from Maseno, Mariwa, Seme and Hollo were collected and cut into small sections (0.5-1cm). The plant sections were then plated onto Triphenyl tetrazolium chloride (TZC) media. Profiling of the pathogen was done morphologicaly using culture techniques; biochemical tests included Gram staining test, Potassium hydroxide test, Catalase oxidase test, Gas production test, Starch hydrolysis test and sugar utilization test. The races were then identified by pathogenicity test on wide host range. All the isolates had fluidal pinkish red centered colonies on TZC media, they were Gram negative, potassium hydroxide solubility positive, produced gas from glucose, ooze test positive and did not hydrolyse starch which is typical of R. solanacerum. R. solanacearum strains from infected tomato plants in Maseno region were race 3 biovar 1 and race 3 biovar 3. All isolates were pathogenic on tomato plants.

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Section: Morphological Profiling Of Ralstonia Solanacearummentioning

confidence: 54%

Isolation and Characterisation of Ralstonia Solanacearum Strains of Tomato Wilt Disease from Maseno, Kenya

Khasabulli

Musyimi

Miruka

et al. 2017

Journal of Asian Scientific Research

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“…Only Philippines have all of biovars 1-4 and here as elsewhere in Asia, biovar 3 predominates in the lowland regions (Hayward, 1991;CABI, 2017a). However, report from Ahmed et al (2013) and Nishat et al (2015) showed that the R. solanacearum isolates causing bacterial wilt of potato in Bangladesh were belonging to Biovar III and Race 3.…”

Section: Distributionmentioning

confidence: 99%

“…Nishat et al (2015) reported the variation in R. solanacearum isolates of potato which was observed among different growing areas of Bangladesh. It was showed that the isolates were belonged to race 3 biovar III.…”

Section: Races and Biovarsmentioning

confidence: 99%

Ralstonia solanacearum: A Threat to Potato Production in Bangladesh

Karim¹,

Hossain²,

Begum³

2018

Fundam Appl Agric

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Potato is one of the most important vegetable crops in Bangladesh which made it 7th producer in the world. This vegetable crop is affected by one of the most notorious soil borne pathogen, Ralstonia solanacearum and caused wilt symptoms in plant and brown rot in potato tuber throughout the world. The pathogen entered into the plants through different natural opening and wounds and is easily disseminated via infected biological material (seeds, vegetatively propagative materials, plants etc.), soil, contaminated irrigation water, surface water, farm equipment etc. and could survive for many years in association with alternate hosts. The bacteria is a quarantine organism being economically a serious problem for potatoes and other major crops in many tropical, subtropical and warmer areas of the world. It has an unusually wide host range including economically important crops (potato, tomato, tobacco, banana, ginger, geranium etc.) and weeds. The disease appeared as rapid and fatal wilting symptoms in the potato plants and vascular browning and/or rotting on tubers. The pathogen can be detected through the isolation of the bacteria on semi-selective TZC and/or selective SMSA (modified) media and through different biochemical tests and/or molecular test. It is a Gram-negative type bacterium, subdivided into five races based on host range and race 3 biovar III is reported in Bangladesh. Direct yield losses caused by R. solanacearum may vary 33 to 90% depending on the different factors such as cultivar, climate, soil type, cropping pattern, strain of the bacteria etc. Due to the latent infection of tuber by this organism, Bangladesh had been facing a temporary embargo on potato export which was imposed by Russia during 2014-15. But, latency and some other survival strategy of the pathogen created much more problems with disease detection, control and dispersal. The biological features of this pathogen makes it unusually successful against the traditional management practices. Therefore, the review focused on the biological abilities of R. solanacearum in relation to dispersal, survival and influences which might be important in designing effective management against the pathogen.

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“…Race 3 affects potatoes and tomatoes but is weakly virulent in other solanaceous crops. Finally, race 4 affects ginger plants in the Philippines, and race 5 affects mulberry plants in China [3].…”

Section: Introductionmentioning

confidence: 99%

“…solanacearum is a gram-negative soil inhabitant that is motile due to the presence of one to four polar flagella. Additionally, is aerobic and can survive in temperatures ranging from 10°C to 41°C and can be found in tropical, subtropical and temperate zones [3]. This pathogen is known for causing bacterial wilt in economically important crops worldwide and can parasitize approximately 250 species belonging to 54 plant families [4,5].…”

Section: Introductionmentioning

confidence: 99%

Effect of Furans and a Pyran on Several Quorum Sensing Factors in Ralstonia solanacearum

Gallego¹,

Giordano²,

Rosero³

2016

J Microb Biochem Technol

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Genetic diversity of the bacterial wilt pathogen Ralstonia solanacearum using a RAPD marker

Cited by 24 publications

References 34 publications

Isolation and Characterisation of Ralstonia Solanacearum Strains of Tomato Wilt Disease from Maseno, Kenya

Isolation and Characterisation of Ralstonia Solanacearum Strains of Tomato Wilt Disease from Maseno, Kenya

Ralstonia solanacearum: A Threat to Potato Production in Bangladesh

Effect of Furans and a Pyran on Several Quorum Sensing Factors in Ralstonia solanacearum

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