Genetic Diversity of the Endemic Xantus’ Hummingbird Using 16 Novel Polymorphic Microsatellite Loci, and Their Cross Amplification between Six Related Species

Novel microsatellites for Cypseloides fumigatus, cross-amplifiable in Streptoprocne zonaris

“…Observed heterozygosities ranged from 0.03 to 0.56 (mean Ho ± SD: 0.16 ± 0.05) and expected heterozygosities from 0.03 to 0.67 (mean He ± SD: 0.25 ± 0.06, Table 1). These heterozygosities were low when compared to that of phylogenetically close species, though the microsatellites used in the reference literature where not the same as those designed and tested in this study (Lance et al 2009, González et al 2010, Oyler-McCance et al 2011, Gutiérrez-Rodriguez et al 2013, Sanvicente et al 2016; Table 2). Several attempts of amplification using two hummingbird microsatellites developed in other studies, Acya3-3 andHxan07 (Gutiérrez-Rodriguez et al 2013, Sanvicente et al 2016), were done by RNB, with different settings of temperature and thermocycling conditions, but resulted in no amplifications for Sooty and Whitecollared Swifts.…”