Genetic diversity of tumorigenic bacteria associated with crown gall disease of raspberry in Serbia

Kuzmanović, Nemanja; Prokić, Anđelka; Ivanović, Milan; Zlatković, Nevena; Gašić, Katarina; Obradović, Aleksa

doi:10.1007/s10658-015-0645-4

Cited by 8 publications

(9 citation statements)

References 47 publications

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“…Agrobacterium biovar 1/ Agrobactrium tumefaciens ), including recently described species Agrobacterium arsenijevicii has been frequently reported on Rubus spp. 4 – 12 . In general, crown gall disease symptoms include formation of tumors on roots and crowns of infected plants.…”

Section: Introductionmentioning

confidence: 99%

Rhizobium tumorigenes sp. nov., a novel plant tumorigenic bacterium isolated from cane gall tumors on thornless blackberry

Kuzmanović

Smalla

Gronow

et al. 2018

Sci Rep

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Four plant tumorigenic strains 932, 1019, 1078T and 1081 isolated from cane gall tumors on thornless blackberry (Rubus sp.) were characterized. They shared low sequence identity with related Rhizobium spp. based on comparisons of 16S rRNA gene (≤98%) and housekeeping genes atpD, recA and rpoB (<90%). Phylogenetic analysis indicated that the strains studied represent a novel species within the genus Rhizobium, with Rhizobium tubonense CCBAU 85046T as their closest relative. Furthermore, obtained average nucleotide identity (ANI) and in silico DNA–DNA hybridization (DDH) values calculated for whole-genome sequences of strain 1078T and related Rhizobium spp. confirmed the authenticity of the novel species. The ANI-Blast (ANIb), ANI-MUMmer (ANIm) and in silico DDH values between strain 1078T and most closely related R. tubonense CCBAU 85046T were 76.17%, 84.11% and 21.3%, respectively. The novel species can be distinguished from R. tubonense based on phenotypic and chemotaxonomic properties. Here, we demonstrated that four strains studied represent a novel species of the genus Rhizobium, for which the name Rhizobium tumorigenes sp. nov. is proposed (type strain 1078T = DSM 104880T = CFBP 8567T). R. tumorigenes is a new plant tumorigenic species carrying the tumor-inducing (Ti) plasmid.

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Section: Introductionmentioning

confidence: 99%

Rhizobium tumorigenes sp. nov., a novel plant tumorigenic bacterium isolated from cane gall tumors on thornless blackberry

Kuzmanović

Smalla

Gronow

et al. 2018

Sci Rep

Self Cite

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“…M a n u s c r i p t Cells are Gram-negative, oxidase positive, aerobic, motile, rod-shaped (0.7-0.9 µm × 1.4-2.6 µm). Good growth occurs on King's medium B [13], YMA [14] and MG agar medium amended with tellurite (K 2 TeO 3 ; 70 µg • ml-1) [19]. Colonies on YMA medium have a diameter of approximately 1 mm after 48 h growth at 27ºC, and are white, circular, convex and glistening.…”

Section: Description Of Agrobacterium Arsenijevicii Sp Novmentioning

confidence: 99%

“…Strains KFB 330 T and KFB 335 were isolated from raspberry (Rubus idaeus) tumors in Serbia in 2012 and 2013, respectively, by isolating on yeast mannitol agar (YMA) [14]. The strain AL51.1 was isolated from a cherry plum (Prunus cerasifera) tumor on 1A+2E medium [26] in Poland in 2009.…”

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confidence: 99%

“…The strain AL51.1 was isolated from a cherry plum (Prunus cerasifera) tumor on 1A+2E medium [26] in Poland in 2009. The strain KFB 330 T was studied previously and its unclear phylogenetic position within A. tumefaciens species complex was pointed out [14].…”

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confidence: 99%

“…In order to check clonality among the three novel strains, rep-PCR fingerprinting with BOX, ERIC and REP primers [37,38] was performed as described before [14]. Additionally, RAPD-PCR analysis with primers OPAR03, OPU19 and OPW20 was used according to procedure described by Puławska and Kałużna [27].…”

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confidence: 99%

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