Genetic diversity of Tunisian accessions ofAegilops geniculataRoth and durum wheats (Triticum durumDesf.) using RAPD markers

Abstract

Abstract.-Genetic diversity of three durum wheat cultivars (Triticum durumDesf.) and thirteen Aegilops geniculata Roth accessions belonging to different regions of Tunisia (in North and Central) was evaluated using random amplified polymorphic DNA (RAPD) markers. Nineteen arbitrary universal primers were used for the amplification of random DNA sequences. Data were analysed with the SIMQUAL program using the NTSYS-pc. Phylogenetic diagram was drowning using the UPGMA algorithm. Results indicate an important inter-specific polymorphism between Aegilops and durum wheat. Ae. geniculata accessions revealed a high level of polymorphism (71.27%) than wheat cultivars (39.76%). Two main groups were represented by cluster and principal component analyses. The first group is formed by Ae. geniculata accessions and the second is constituted only by durum wheat cultivars. Consequently, RAPD markers separate clearly Ae. geniculata accessions and durum wheat cultivars.Key words : Aegilops geniculata -Triticum durum -polymorphism -RAPD. Résumé.-La diversité génétique de trois cultivars du blé dur (Triticum durumDesf.) et de treize accessions d'Aegilops geniculata Roth originaires de diffé-rentes régions de la Tunisie (nord et centre) a été évaluée en utilisant la technique RAPD. 19 amorces universelles arbitraires ont été utilisées pour l'amplification aléatoire des séquences de l'ADN. Les données statistiques ont été soumises au programme SIMQUAL du logiciel NTSYS-pc. Le diagramme phylogénétique a été réalisé par le sous-programme d'analyse UPGMA. Les résultats montrent un important polymorphisme interspécifique d'Ae. geniculata et de blé dur. Les accessions d'Ae. geniculata ont révélé un niveau élevé de polymorphisme (71.27%) par rapport à celui du blé (39.76%). Deux principaux groupes sont représentés par le dendrogramme de similarité et l'analyse en composantes principales. Le premier est formé par les accessions d'Ae. geniculata alors que le second n'est formé que par les cultivars du blé dur. En consé-quence, les marqueurs RAPD ont permis de séparer clairement les accessions d'Ae. geniculata et celles de cultivars du blé dur.

Karyological study and meiotic analysis of four species ofAegilops(Poaceae) in Algeria

“…However, other molecular approaches have been developed to obtain better knowledge of the phenomena of speciation and evolution in these species, e.g. the study of reserve proteins (Fernández-Calvín and Orellana 1990;Rodriguez-Quijano et al 2000;Sun et al 2006) and DNA polymorphism (Zhang et al 1996;Zaharieva et al 2001;Sasanuma et al 2004;Salina et al 2006;Haider and Nabulsi 2008;Mahjoub et al 2010;Giraldo et al 2016).…”

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“…They found that in the majority of polyploid Aegilops species, the genome is variously modified as compared to their ancestors. However, other molecular approaches have been developed, such as the study of reserved proteins (Fernandez-Calvin, Orellana, 1990;Rodriguez-Quijano et al, 2000;Sun et al, 2006) and DNA polymorphism (Zhang et al, 1996;Zaharieva et al, 2001;Sasanuma et al, 2004;Salina et al, 2006;Haider et al, 2008;Mahjoub et al, 2010;Giraldo et al, 2016) for better knowledge of speciation processes and genomic evolution in these species.…”

Genetic diversity of ribosomal loci (5S and 45S rDNA) and pSc119.2 repetitive DNA sequence among four species of Aegilops (Poaceae) from Algeria

“…They found that in the majority of polyploid Aegilops species, the genome is variously modified as compared to their ancestors. However, other molecular approaches have been developed, such as the study of reserved proteins (Fernandez-Calvin, Orellana, 1990;Rodriguez-Quijano et al, 2000;Sun et al, 2006) and DNA polymorphism (Zhang et al, 1996;Zaharieva et al, 2001;Sasanuma et al, 2004;Salina et al, 2006;Haider et al, 2008;Mahjoub et al, 2010;Giraldo et al, 2016) for better knowledge of speciation processes and genomic evolution in these species.…”