Genetic diversity of two perch Perca fluviatilis populations of the Latgale region

Abstract

Molecular markers based on retrotransposons possibility of integration into genomes of many organisms are commonly used for genetic analysis of different species. The aim of this study was to test possibility of use those markers in perch and detect genetic diversity of populations of two lakes of the Latgale region of Latvia: Cirišs and Sventes. The distance between the lakes is nearly 60 km, they belong to the same Daugava river basin but are different from ecological point of view. Forty two blood samples of <em>Perca fluviatilis </em>were collected altogether. Extracted DNA was analyzed using inter-PBS amplification technique with specifically selected retrotransposon-based primers and agarose gel electrophoresis. Data of genetic diversity of lakes were calculated by POPGENE and NTSYS software. In total, 127 loci were found in two populations, 81 of them were polymorphic. In spite of rather small distance between lakes Cirišs and Sventes, several differences in genetic variation of populations of two lakes were found. For example, the number of polymorphic loci in samples from Sventes lake was 61, but from Cirišs lake only 47. A unique allele was found in Lake Sventes. Genetic similarity and distance between populations of lakes Cirišs and Sventes was 0.9288 and 0.0739 respectively. Results proved that molecular markers based on retrotransposons PBS regions can be very useful to test genetic diversity of fish populations.

Genetic characterization of selected populations of European perch, Perca fluviatilis (Actinopterygii: Perciformes: Percidae), in the waters of north-western Poland with recommendations for fishing and stocking policies

“…These are typical periodic migrations for grazing and spawning. Migrations of perch from Pomeranian Bay to the Szczecin Lagoon are also confirmed by analyses conducted by Garbacik-Wesołowska and Boberski (2000). Perch inhabiting the coastal zone of the Baltic Sea probably pass the longest distances among the known perch stocks-their range is up to 80 km (Król 2008).…”

“…They can be defined as forms with a highly arched back in reservoirs rich in food, and elongated forms with a poorly arched back in reservoirs poor in food. A study by Fokina et al (2015) revealed a possibility of genetic diversity of two perch populations whose geographical area of occurrence was limited to the catchment area of one river in Latvia. The distance between the two lakes from which the fish were sampled was approx.…”

Genetic Structure of Perch Perca Fluviatilis (L.) Populations in Latvian Rivers that are Fragmented (Daugava) and Non-Fragmented (Lielupe) By Hydroelectric Dams

“…Studies have been conducted on the consequences of overexploitation and subsequent population recovery of Eurasian perch (Perca fluviatilis L.) populations (Pukka et al, 2016), and the impacts of a nuclear power plant on the genetic structure of perch populations as an intensively exploited fish species (Ragauskas et al, 2014). It was shown that genetic differences are not related to geo-graphical distances, but probably depend on ecological characteristics (Fokina et al, 2015). Eurasian perch was used for investigations on the relative importance of genetic variation and plasticity on phenotypic variation (Svanbäck and Eklöv, 2006).…”