Genetic diversity of wheat (Triticum aestivum L.) plants-regenerants produced by anther culture

Grauda, Dace; Žagata, Kârlis; Lanka, G.; Strazdiņa, Vija; Fetere, V.; Ļisina, Nadežda; Krasņevska, Nikole; Fokina, Oksana; Miķelsone, Andra; Ornicāns, Reinis; Belogrudova, Inta; Rashal, Isaak

doi:10.18699/vj16.176

Cited by 10 publications

(10 citation statements)

References 30 publications

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“…Regarding spontaneously doubled haploid plants, the situation is the opposite of the haploid plant. The results are supported by the findings of Grauda et al (2016) who showed that genotype significantly affected haploid plant and spontaneous doubled-haploid plants developed from green plants.…”

Section: Discussionsupporting

confidence: 78%

Enhancement of Androgenesis and Plant Regeneration From Wheat Anther Culture by Seed Pre-Sowing Gamma Irradiation

Bilgin

SARIER

Başer³

et al. 2022

Tekirdağ Ziraat Fakültesi Dergisi

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Combination breeding and mutation breeding are widely used methods in plant breeding. Intensive studies are carried out on biotechnological methods that will allow obtaining homozygous lines in a short time in populations obtained with these two techniques. Numerous studies have been carried out by different researchers on mutation breeding in wheat. There are few studies on anther culture response in mutagen-treated genotypes. Two different bread wheat promising advanced lines were used as the material in the study, in which the possibilities of combining mutation breeding with anther culture through ionizing radiation in high quality wheat breeding were investigated. In the study, the responses of advanced bread wheat mutant lines to anther culture, to which eight different doses of gamma rays (0, 100, 150, 200, 250, 300, 350, 400 Gy) were applied, including the control, were investigated. There are significant differences between genotypes and irradiation doses for all traits studied. It has been shown that it is possible to decrease albinism and increase the response of anther culture with dose-dependent gamma irradiation depending on bread wheat varieties. In the multiple comparison test to classify the difference between doses; statistically, 150 gray dose 5.60 is in the first statistical class and in the first place with the number of transferred green plants. After that, it ranks second in the same class with the number of green plants transferred with a 300 gray dose of 5.21. In the total number of regenerated green plants excluding controls (888), 635 unit (71.5%) and 205 unit (23.1%) haploids and spontaneous double haploid plants were obtained, respectively. In the study integrated into the bread wheat breeding program, a total of 205 spontaneous double haploid mutant lines were produced. According to the data obtained, it was shown that the gamma ray doses of 150 and 200 Gy had a significant stimulation effect on all parameters studied and ultimately the success index of anther culture in bread wheat compared to control.

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Section: Discussionsupporting

confidence: 78%

Enhancement of Androgenesis and Plant Regeneration From Wheat Anther Culture by Seed Pre-Sowing Gamma Irradiation

Bilgin

SARIER

Başer³

et al. 2022

Tekirdağ Ziraat Fakültesi Dergisi

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“…However, both sporogenesis and gametogenesis of Arabidopsis thaliana were observed in flower buds exhibiting visual filament [26]. Using buds whose size correlated to a suitable stage of pollen development has been one of the crucial factors determining the success of in vitro microspore or anther culture [10], and different stages were required for different species [9,11]. Hence, flower buds of ≥ 1.6 mm in diameter presenting haploid unicellular microspore or bicellular pollen might be suitable and recommended for the haploid plant induction of J. curcas.…”

Section: Male and Female Gametophyte Development In Relation To Bud Diameter And Morphology Male Gametophyte (Pollen) Developmentmentioning

confidence: 99%

“…Accordingly, haploid and double haploid embryos are required, because they provide homozygous genotypes necessary for breeding programs [8]. Haploid embryos can be induced through anther [9] or unfertilized eggs [8]. An appropriate stage of microspore and embryo sac, which is one of the most critical parameters for the success of the haploid culture, varies among the species [8,10,11].…”

Section: Introductionmentioning

confidence: 99%

Developmental Evidence of Male and Female Gametophytes of Jatropha curcas L.: Pollen Capability and Stigma Receptivity

Nuanjunkong

Tongurai

Meesawat

2018

Walailak J Sci & Tech

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Physic nut (Jatropha curcas L.), a promising alternative resource for biodiesel production, produces restrictively a seed yield and has a low level of genetic variation, causing a barrier to improve the oil performance. Therefore, better understanding of reproductive biology is crucially important for breeding programs to achieve commercial viability in the future. The developmental features related to floral bud diameter and floral morphology of male and female gametophytes, by means of light and scanning electron microscopes, and their capability were investigated. It was found that bud diameter could be used as criteria for identification of pollen and embryo sac stages. Pollen capability and stigma receptivity showed an inverse S-shape with time after flowering. The highest pollen viability assessed by FCR (93.20 - 98.60 %), TTC (78.80 - 87.00 %), and germination test (43.40 %) were obtained at 0 - 8, 0 - 12, and 0 HAF, respectively. Moderately linear correlations were exhibited between viability and germination test (R2 = 0.57 - 0.62). Stigma receptivity was the highest (97.22 - 100 %) on the first three days.

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“…MARCINIAK et al (2003) reported that hybrids were generally more responsive than their parents. CHAUDHARY et al (2003), GRAUDA et al (2016) and DAGUSTU (2008) found that respectively, additive, dominant, and epistatic gene effects play a role in the inheritance of androgenetic traits. Heterosis for anther response is changed from genotype to genotype (AL-ASHKAR, 2014).…”

Section: Introductionmentioning

confidence: 99%

Line × tester analyses for anther culture response of bread wheat (Triticum aestivum L.)

Kutlu¹,

Sirel²,

Yorgancilar³

et al. 2019

Genetika

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The aim of this study was to investigate the gene effects that play a role in the inheritance of the anther culture response of the F1 and F2 populations obtained from the hybridization between the seven doubled haploid (DH) wheat line and four bread wheat cultivar using line × tester method as well as, to determine suitable parents and promising hybrids. Eleven parents and their twenty-eight hybrid combinations were examined for androgenic properties related to anther culture response such as the number of callus, green, albino, haploid, spontaneous DH and fertile plants. The obtained data were evaluated by the combining ability analysis calculated using the line × tester method and by estimating the heritability degrees. According to the results, for all traits examined, the non-additive gene effects were found as significant and narrow sense heritability at a low level. Significant combining ability effects have been identified for some parents and hybrids regarding traits examined. DH19, DH22, and Harmankaya-99 have been identified as suitable parents to develop the androgenic traits, while crosses formed between them have been determined as promising. A total of 444 plants (142 F1, 302 F2) were transferred to the greenhouse from the plants obtained from the anther culture, and 205 (68 in F1, 137 in F2) were fertile. Ninety-three of the fertile plants were obtained from

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Genetic diversity of wheat (Triticum aestivum L.) plants-regenerants produced by anther culture

Cited by 10 publications

References 30 publications

Enhancement of Androgenesis and Plant Regeneration From Wheat Anther Culture by Seed Pre-Sowing Gamma Irradiation

Enhancement of Androgenesis and Plant Regeneration From Wheat Anther Culture by Seed Pre-Sowing Gamma Irradiation

Developmental Evidence of Male and Female Gametophytes of Jatropha curcas L.: Pollen Capability and Stigma Receptivity

Line × tester analyses for anther culture response of bread wheat (Triticum aestivum L.)

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