Genetic Diversity of Winter Wheat (Triticum aestivum L.) Revealed by SSR Markers

Akfirat, Funda Senturk; Uncuoğlu, Ahu Altınkut

doi:10.1007/s10528-012-9557-6

Cited by 29 publications

(11 citation statements)

References 28 publications

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“…Advances in desired traits must be achieved without negative effects on valuable others. The genetic diversity of crops has decreased primarily as a consequence of breeding, including the repeated use of local germplasm and the adoption of breeding schemes that do not favour genetic recombination (Akfirat and Uncuoglu, 2013). Mutagenesis is a powerful instrument to broaden genetic variation and it has been used for yield increase, but for improvement of grain nutritional quality have been much less studied.…”

Section: Introductionmentioning

confidence: 99%

New Spring Wheat Mutation Resources with Improved Grain Quality, Metals Bioavailability and Yield Components

Kenzhebayeva¹,

Abekova²,

Zhang³

et al. 2017

American Journal of Agricultural and Biological Sciences

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Genetic variability in micronutrients is limited in wheat varieties. To broaden variation, seeds of cv. Eritrospermum-35 were irradiated with 100 and 200 Gy using 60 Co, the treated seeds were grown to M 7 generation with successive selection of the highest yield lines in each generation. Selected lines were evaluated for yield and grain quality traits such as iron, zinc and phytic acid concentrations, calculation of molar ratios of PA:Fe and PA:Zn. A number of mutant lines had 2 to 3 times more iron and zinc concentrations and lower phytic acid concentration (1.1-3.5 times) and higher protein content (11.2-12.4%) relative to the parent were identified. Some M 7 lines showed significantly larger grain weight (1.3-1.5 times) and number per spike (2.0-2.1 times) than the parent. There were significant correlations between zinc and iron concentrations, between grain protein content, zinc, iron and phytic acid concentrations and grain weight per spike, mainly in 100 Gy-dosed lines. The TaPhyllc cDNAs nucleotide and protein sequence similarities between parent and these M 7 lines varied from 28.2% and 30.2% to 45.7% and 56.5%, respectively. The results clearly indicate that variability in grain nutrients and metals bioavailability in mutation resource is potential in wheat improving nutritional outcomes and overcome malnutrition.

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Section: Introductionmentioning

confidence: 99%

New Spring Wheat Mutation Resources with Improved Grain Quality, Metals Bioavailability and Yield Components

Kenzhebayeva¹,

Abekova²,

Zhang³

et al. 2017

American Journal of Agricultural and Biological Sciences

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“…As a key first step, comprehensive understanding of genetic backgrounds of sturgeon species is required. With developments in molecular techniques, studies on genetic diversity, population structure, and genetic variation, as well as marker assisted selective breeding based on simple sequence repeat (SSR) and single nucleotide polymorphism techniques (Hu et al, 2010;Senturk Akfirat and Uncuoglu, 2013;Zhang et al, 2013) have been successfully performed. However, the available genetic information is insufficient for unannotated regions; therefore, large numbers of novel molecular markers for the sturgeon database need to be updated, which may provide more suitable messages for the identification of selection breeding in sturgeon.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome analysis and de novo annotation of the critically endangered Amur sturgeon (Acipenser schrenckii)

Zhang¹,

Hy²,

Lm³

et al. 2016

Genet. Mol. Res.

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ABSTRACT. The aim of this study was to provide comprehensive insights into the genetic background of sturgeon by transcriptome study. We performed a de novo assembly of the Amur sturgeon Acipenser schrenckii transcriptome using Illumina Hiseq 2000 sequencing. A total of 148,817 non-redundant unigenes with base length of approximately 121,698,536 bp and ranges from 201 to 26,789 bp were obtained. All the unigenes were classified into 3368 distinct categories and 145,449 singletons by homologous transcript cluster analysis. In all, 46,865 (31.49%) unigenes showed homologous matches with Nr database and 32,214 (21.65%) unigenes were matched to Nt database. In total, 24,862 unigenes were categorized into significantly enriched 52 function groups by GO analysis, and 38,436 unigenes were classified into 25 groups by KOG prediction, as well as 128 enriched KEGG pathways were identified by 45,598 unigenes (P < 0.05). Subsequently, a total of 19,860 SSRs markers were identified with the abundant di- nucleotide type (10,658; 53.67%) and the most AT/TA motif repeats (2689; 13.54%). A total of 1341 conserved lncRNAs were identified by a customized pipeline. Our study provides new sequence and function information for A. schrenckii, which will be the basis for further genetic studies on sturgeon species. The huge number of potential SSRs and putatively conserved lncRNAs isolated by the transcriptome also shed light on research in many fields, including the evolution, conservation management, and biological processes in sturgeon.

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“…The effective protection of H. chinensis population needs comprehensive understanding of the genetic background of the animal populations. With the development of molecular techniques, they have enabled the study of genetic diversity, population structure and genetic variation as well as marker assisted selection breeding, such as simple sequence repeat (SSR) one of the most useful Mendelian markers [8]–[10] and single nucleotide polymorphism (SNP) [11]. Taken together, a fast and cost-efficient approach to exploit important candidate genes involved in local adaptation, immune system and reproduction, as well as molecular markers for H. chinensis is required.…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic Analysis of Endangered Chinese Salamander: Identification of Immune, Sex and Reproduction-Related Genes and Genetic Markers

et al. 2014

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BackgroundThe Chinese salamander (Hynobius chinensis), an endangered amphibian species of salamander endemic to China, has attracted much attention because of its value of studying paleontology evolutionary history and decreasing population size. Despite increasing interest in the Hynobius chinensis genome, genomic resources for the species are still very limited. A comprehensive transcriptome of Hynobius chinensis, which will provide a resource for genome annotation, candidate genes identification and molecular marker development should be generated to supplement it.Principal FindingsWe performed a de novo assembly of Hynobius chinensis transcriptome by Illumina sequencing. A total of 148,510 nonredundant unigenes with an average length of approximately 580 bp were obtained. In all, 60,388 (40.66%) unigenes showed homologous matches in at least one database and 33,537 (22.58%) unigenes were annotated by all four databases. In total, 41,553 unigenes were categorized into 62 sub-categories by BLAST2GO search, and 19,468 transcripts were assigned to 140 KEGG pathways. A large number of unigenes involved in immune system, local adaptation, reproduction and sex determination were identified, as well as 31,982 simple sequence repeats (SSRs) and 460,923 putative single nucleotide polymorphisms (SNPs).ConclusionThis dataset represents the first transcriptome analysis of the Chinese salamander (Hynobius chinensis), an endangered species, to be also the first time of hynobiidae. The transcriptome will provide valuable resource for further research in discovery of new genes, protection of population, adaptive evolution and survey of various pathways, as well as development of molecule markers in Chinese salamander; and reference information for closely related species.

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Genetic Diversity of Winter Wheat (Triticum aestivum L.) Revealed by SSR Markers

Cited by 29 publications

References 28 publications

New Spring Wheat Mutation Resources with Improved Grain Quality, Metals Bioavailability and Yield Components

New Spring Wheat Mutation Resources with Improved Grain Quality, Metals Bioavailability and Yield Components

Transcriptome analysis and de novo annotation of the critically endangered Amur sturgeon (Acipenser schrenckii)

Transcriptomic Analysis of Endangered Chinese Salamander: Identification of Immune, Sex and Reproduction-Related Genes and Genetic Markers

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