Genetic diversity within the species <i>Arachis duranensis</i> Krapov. &amp;W.C. Gregory, a possible progenitor of cultivated peanut

Stalker, H. T.; Dhesi, J. S.; Kochert, Gary

doi:10.1139/g95-158

Cited by 32 publications

(19 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previous research found a low frequency (0.01-0.26/PMC) of multivalents in 12 of 27 hybrids from crosses of A . duranensis accessions [69], and quadrivalents were identified in all these 12 hybrids, which likely represents chromosome translocations within this species. The asymmetrical chromosomes found in different accessions, furthermore, indicated the presence of translocations.…”

Section: Resultsmentioning

confidence: 99%

Comparative mapping in intraspecific populations uncovers a high degree of macrosynteny between A- and B-genome diploid species of peanut

et al. 2012

View full text Add to dashboard Cite

BackgroundCultivated peanut or groundnut (Arachis hypogaea L.) is an important oilseed crop with an allotetraploid genome (AABB, 2n = 4x = 40). Both the low level of genetic variation within the cultivated gene pool and its polyploid nature limit the utilization of molecular markers to explore genome structure and facilitate genetic improvement. Nevertheless, a wealth of genetic diversity exists in diploid Arachis species (2n = 2x = 20), which represent a valuable gene pool for cultivated peanut improvement. Interspecific populations have been used widely for genetic mapping in diploid species of Arachis. However, an intraspecific mapping strategy was essential to detect chromosomal rearrangements among species that could be obscured by mapping in interspecific populations. To develop intraspecific reference linkage maps and gain insights into karyotypic evolution within the genus, we comparatively mapped the A- and B-genome diploid species using intraspecific F2 populations. Exploring genome organization among diploid peanut species by comparative mapping will enhance our understanding of the cultivated tetraploid peanut genome. Moreover, new sources of molecular markers that are highly transferable between species and developed from expressed genes will be required to construct saturated genetic maps for peanut.ResultsA total of 2,138 EST-SSR (expressed sequence tag-simple sequence repeat) markers were developed by mining a tetraploid peanut EST assembly including 101,132 unigenes (37,916 contigs and 63,216 singletons) derived from 70,771 long-read (Sanger) and 270,957 short-read (454) sequences. A set of 97 SSR markers were also developed by mining 9,517 genomic survey sequences of Arachis. An SSR-based intraspecific linkage map was constructed using an F2 population derived from a cross between K 9484 (PI 298639) and GKBSPSc 30081 (PI 468327) in the B-genome species A. batizocoi. A high degree of macrosynteny was observed when comparing the homoeologous linkage groups between A (A. duranensis) and B (A. batizocoi) genomes. Comparison of the A- and B-genome genetic linkage maps also showed a total of five inversions and one major reciprocal translocation between two pairs of chromosomes under our current mapping resolution.ConclusionsOur findings will contribute to understanding tetraploid peanut genome origin and evolution and eventually promote its genetic improvement. The newly developed EST-SSR markers will enrich current molecular marker resources in peanut.

show abstract

Section: Resultsmentioning

confidence: 99%

Comparative mapping in intraspecific populations uncovers a high degree of macrosynteny between A- and B-genome diploid species of peanut

et al. 2012

View full text Add to dashboard Cite

show abstract

“…RAPD data also suggested they are very closely related [37]. F 1 hybrids between accession GK 12787 of A. pintoi and accession GKP 10538 of A. repens , which represent the extreme types, had 86.8% pollen fertility [55], which is higher than the level of pollen fertility found in intraespecific hybrids of crosses between accessions of some other Arachis species [45,60]. …”

Section: Resultsmentioning

confidence: 99%

Phylogenetic relationships in genus Arachis based on ITS and 5.8S rDNA sequences

et al. 2010

View full text Add to dashboard Cite

BackgroundThe genus Arachis comprises 80 species and it is subdivided into nine taxonomic sections (Arachis, Caulorrhizae, Erectoides, Extranervosae, Heteranthae, Procumbentes, Rhizomatosae, Trierectoides, and Triseminatae). This genus is naturally confined to South America and most of its species are native to Brazil. In order to provide a better understanding of the evolution of the genus, we reconstructed the phylogeny of 45 species using the variation observed on nucleotide sequences in internal transcribed spacer regions (ITS1 and ITS2) and 5.8 S of nuclear ribosomal DNA.ResultsIntraspecific variation was detected, but in general it was not enough to place accessions of the same species in different clades. Our data support the view that Arachis is a monophyletic group and suggested Heteranthae as the most primitive section of genus Arachis. The results confirmed the circumscriptions of some sections (Caulorrhizae, Extranervosae), but raised questions about others. Sections Erectoides, Trierectoides and Procumbentes were not well defined, while sections Arachis and Rhizomatosae seem to include species that could be moved to different sections. The division of section Arachis into A and B genome species was also observed in the phylogenetic tree and these two groups of species may not have a monophyletic origin. The 2n = 2x = 18 species of section Arachis (A. praecox, A. palustris and A. decora) were all placed in the same clade, indicating they are closely related to each other, and their genomes are more related to B genome than to the A genome. Data also allowed insights on the origin of tetraploid A. glabrata, suggesting rhizome appeared twice within the genus and raising questions about the placement of that species in section Rhizomatosae.ConclusionThe main clades established in this study in general agreed with many other studies that have used other types of evidences and sets of species, being some of them included in our study and some not. Thus, the relationships established can be a useful framework for future systematic reviews of genus Arachis and for the selection of species to pre-breeding programs.

show abstract

“…The data suggested that cross-pollination happens in some Arachis species. Evidences of cross-pollination in A. duranensis were found when different accessions were analyzed using RFLP [37]. The extensive polymorphism detected within accessions of A. cardenasii using cDNA and seed storage proteins probes [5,38,39] has also been suggested to be related to high frequency of cross-pollination.…”

Section: Resultsmentioning

confidence: 99%

Characterization and transferability of microsatellite markers of the cultivated peanut (Arachis hypogaea)

et al. 2007

View full text Add to dashboard Cite

Background: The genus Arachis includes Arachis hypogaea (cultivated peanut) and wild species that are used in peanut breeding or as forage. Molecular markers have been employed in several studies of this genus, but microsatellite markers have only been used in few investigations. Microsatellites are very informative and are useful to assess genetic variability, analyze mating systems and in genetic mapping. The objectives of this study were to develop A. hypogaea microsatellite loci and to evaluate the transferability of these markers to other Arachis species.

show abstract

Genetic diversity within the species Arachis duranensis Krapov. &W.C. Gregory, a possible progenitor of cultivated peanut

Cited by 32 publications

References 28 publications

Comparative mapping in intraspecific populations uncovers a high degree of macrosynteny between A- and B-genome diploid species of peanut

Comparative mapping in intraspecific populations uncovers a high degree of macrosynteny between A- and B-genome diploid species of peanut

Phylogenetic relationships in genus Arachis based on ITS and 5.8S rDNA sequences

Characterization and transferability of microsatellite markers of the cultivated peanut (Arachis hypogaea)

Contact Info

Product

Resources

About