Genetic effects analysis of myeloid leukemia factor 2 and T cell receptor-β on resistance to coccidiosis in chickens

Kim, E.-S.; Hong, Yeong Ho; Lillehoj, Hyun S.

doi:10.3382/ps.2009-00351

Cited by 9 publications

(17 citation statements)

References 37 publications

(43 reference statements)

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“…Single marker and haplotype-based tests were applied to determine the associations between SNPs and the parameters of coccidiosis resistance including body weight and Box-Cox transformed oocyst shedding. The maximum additive genetic effect on disease resistance of a SNP in MLF2 was explained by body weight ( p = 0.0002) and this SNP of MLF2 also significantly associated with body weight was also associated with fecal oocyst shedding [6]. This was confirmed using Box-Cox transformed oocyst shedding data in this study.…”

Section: Resultssupporting

confidence: 74%

“…Ten SNPs were identified in the zyxin , 12 in the TCR-β and 4 in the MLF2 gene [5,6]. At various times following experimental infection of the F 2 generation with Eimeria maxima , body weights, fecal oocyst shedding, and biochemical parameters were measured as parameters of coccidiosis resistance [3].…”

Section: Resultsmentioning

confidence: 99%

“…PCR product was sequenced with internal primers using an ABI 3730 DNA analyzer (Applied Biosystem, Foster City, CA). SNP was genotyped by genomic DNA sequencing with the same gene-specific internal primers [5,6]. Then, haplotype phase was decided based on pedigree information.…”

Section: Methodsmentioning

confidence: 99%

“…The current analysis was conducted to identify the parental line specific haplotype of MLF2 . Although MLF2 explained variation of body weight affected by coccidiosis, the association of causative mutation was not obviously identified [6]. Thus, this study was suggested to identify segregation of MLF2 allele associated with coccidiosis resistance for practical application and future study for identifying causative mutation.…”

Section: Introductionmentioning

confidence: 97%

“…The resistance to avian coccidiosis QTL has been identified near two microsatellite markers on chromosome 1 [3,4]. Associations between parameters of resistance to coccidiosis and single nucleotide polymorphisms (SNPs) in 3 candidate genes located around QTL on chromosome 1 ( Zyxin , TCR-β , MLF2 ) were determined [5,6]. These studies showed the SNPs in MLF2 are the most probable locus associated with coccidiosis resistance in chickens.…”

Section: Introductionmentioning

confidence: 99%

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Identification of parental line specific effects of MLF2 on resistance to coccidiosis in chickens

2011

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BackgroundMLF2 was the candidate gene associated with coccidiosis resistance in chickens. Although single marker analysis supported the association between MLF2 and coccidiosis resistance, causative mutation relevant to coccidiosis was not identified yet. Thus, this study suggested segregation analysis of MLF2 haplotype and the association test of the other candidate genes using improved data transformation.ResultsA haplotype probably originated from one parental line was found out of 4 major haplotypes of MLF2. Frequency of this haplotype was 0.2 in parental chickens and its offspring in 12 families. Allele substitution effect of the MLF2 haplotype originated from a specific line was associated with increased body weight and fecal egg count explaining coccidiosis resistance. Nevertheless Box-Cox transformation was able to improve normality; association test did not produce obvious different results compared with analysis with log transformed phenotype.ConclusionAllele substitution effect analysis and classification of MLF2 haplotype identified the segregation of haplotype associated with coccidiosis resistance. The haplotype originated from a specific parental line was associated with improving disease resistance. Estimating effect of MLF2 haplotype on coccidiosis resistance will provide useful information for selecting animals or lines for future study.

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Section: Resultssupporting

confidence: 74%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

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Identification of parental line specific effects of MLF2 on resistance to coccidiosis in chickens

2011

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Genomic heterozygosity is associated with parasite abundance, but the effects are not mediated by host condition

2022

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Whether, when, and how genetic diversity buffers individuals and populations against infectious disease risk is a critical and open question for understanding wildlife disease and zoonotic disease risk. Several, but not all, studies have found negative relationships between infection and heterozygosity in wildlife. Since they can host multiple zoonotic infections, we sampled a population of wild deer mice ( Peromyscus maniculatus ), sequenced their genomes, and examined their fecal samples for coccidia and nematode eggs. We analyzed coccidia infection status, abundance, and coinfection status in relation to per-locus and per-individual measures of heterozygosity, as well as identified SNPs associated with infection status. Since heterozygosity might affect host condition, and condition is known to affect immunity, it was included as a co-variate in the per-individual analyses and as response variable in relation to heterozygosity. Not only did coccidia-infected individuals have lower levels of genome-wide per-locus diversity across all metrics, but we found an inverse relationship between genomic diversity and severity of coccidia infection. We also found weaker evidence that coinfected individuals had lower levels of private allelic variation than all other groups. In the per-individual analyses, relationships between heterozygosity and infection were marginal but followed the same negative trends. Condition was negatively correlated with infection, but was not associated with heterozygosity, suggesting that effects of heterozygosity on infection were not mediated by host condition in this system. Association tests identified multiple loci involved in the inflammatory response, with a particular role for NF-κB signaling, supporting previous work on the genetic basis of coccidia resistance. Taken together, we find that increased genome-wide neutral diversity, the presence of specific genetic variants, and improved condition positively impact infection status. Our results underscore the importance of considering host genomic variation as a buffer against infection, especially in systems that can harbor zoonotic diseases. Supplementary Information The online version contains supplementary material available at 10.1007/s10682-022-10175-8.

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The long view: a selective review of 40 years of coccidiosis research

Shirley¹,

Lillehoj

2012

Avian Pathology

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This selective review of 40 years of coccidiosis research is one of a number on important diseases of poultry to celebrate the 40th anniversary of the birth of Avian Pathology, the journal of the World Veterinary Poultry Association, and is written for the non-specialist. The intention is to provide a flavour of the field problems and intellectual challenges, with emphasis in the areas of immunology and vaccinology that drove research in the 1970s, and to reflect on research progress since.

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Genetic effects analysis of myeloid leukemia factor 2 and T cell receptor-β on resistance to coccidiosis in chickens

Cited by 9 publications

References 37 publications

Identification of parental line specific effects of MLF2 on resistance to coccidiosis in chickens

Identification of parental line specific effects of MLF2 on resistance to coccidiosis in chickens

Genomic heterozygosity is associated with parasite abundance, but the effects are not mediated by host condition

The long view: a selective review of 40 years of coccidiosis research

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