Genetic effects of leptin receptor (LEPR) polymorphism on litter size in a Black Bengal goat population

Alim, Md. Abdul; Hossain, Mridha Md. Kamal; Nusrat, J.; Rubaya,; Salimullah, Md.; Zhou, Shuhong; Alam, Jahangir

doi:10.1163/15707563-00001079

Cited by 8 publications

(6 citation statements)

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“…It is noteworthy that Tibetan goat has other characteristics and traits besides plateau adaptability, such as slow growth, small body size and low fecundity. So, some relative genes were detected, the most special gene was LEPR , which not only related to hypoxic, but was also associated with reproductive and fattiness traits in goats and sheep [ 47 , 48 ]. PROP1 (PROP paired-like homeobox 1) ( di value = 15.53), another strongly selected gene, proved to be associated with growth traits of goat and sheep [ 49 , 50 ].…”

Section: Resultsmentioning

confidence: 99%

Selection Signatures Analysis Reveals Genes Associated with High-Altitude Adaptation in Tibetan Goats from Nagqu, Tibet

Jin

Lü

Fei

et al. 2020

Animals

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Tibetan goat is an ancient breed, which inhabits the adverse conditions of the plateaus in China. To investigate the role of selection in shaping its genomes, we genotyped Tibetan goats (Nagqu Prefecture, above 4500 m) and three lowland populations (Xinjiang goats, Taihang goats and Huanghuai goats). The result of PCA, neighbor-joining (N-J) tree and model-based clustering showed that the genetic structure between the Tibetan goat and the three lowland populations has significant difference. As demonstrated by the di statistic, we found that some genes were related to the high-altitude adaptation of Tibetan goats. Functional analysis revealed that these genes were enriched in the VEGF (vascular endothelial growth factor) signaling pathway and melanoma, suggesting that nine genes (FGF2, EGFR, AKT1, PTEN, MITF, ENPEP, SIRT6, KDR, and CDC42) might have important roles in the high-altitude adaptation of Nagqu Tibetan goats. We also found that the LEPR gene was under the strongest selection (di value = 16.70), and it could induce upregulation of the hypoxic ventilatory response. In addition, five genes (LEPR, LDB1, EGFR, NOX4 and FGF2) with high di values were analyzed using q-PCR. Among them, we found that LEPR, LDB1 and FGF2 exhibited higher expression in the lungs of the Tibetan goats; LEPR, EGFR and LDB1 exhibited higher expression in the hearts of the Huanghuai goat. Our results suggest that LEPR, LDB1, EGFR and FGF2 genes may be related to the high-altitude adaptation of the goats. These findings improve our understanding of the selection of the high-altitude adaptability of the Nagqu Tibetan goats and provide new theoretical knowledge for the conservation and utilization of germplasm resources.

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Section: Resultsmentioning

confidence: 99%

Selection Signatures Analysis Reveals Genes Associated with High-Altitude Adaptation in Tibetan Goats from Nagqu, Tibet

Jin

Lü

Fei

et al. 2020

Animals

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“…This biological pathway is vital in goat breeds in Kenya to maintain adequate energy production and activity in their harsh environment, especially during the lactation period. The LEPR gene is also related to hypoxic in Tibetan goats ( Jin et al, 2020 ) and reproductive and fatness traits in goats and sheep ( Alim et al, 2019 ; Gunawan et al, 2019 ). The parathyroid hormone synthesis, secretion, and action of the KEGG pathway is associated with GNAI2 , PDE4B , and RHOA genes identified in this study.…”

Section: Discussionmentioning

confidence: 99%

Selection Signature Analyses Revealed Genes Associated With Adaptation, Production, and Reproduction in Selected Goat Breeds in Kenya

et al. 2022

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Artificial and natural selection in livestock is expected to leave unique footprints on their genomes. Goat breeds in Kenya have evolved for survival, breeding, and production in various harsh ecological areas, and their genomes are likely to have acquired unique alleles for adaptation to such diverse production environments and other traits of economic importance. To investigate signals of selection for some selected goat breeds in Kenya, Alpine (n = 29), Galla (n = 12), Saanen (n = 24), and Toggenburg (n = 31) were considered. A total of 53,347 single-nucleotide polymorphisms (SNPs) generated using the Illumina GoatSNP50 BeadChip were analyzed. After quality control, 47,663 autosomal single-nucleotide polymorphisms remained for downstream analyses. Several complementary approaches were applied for the following analyses: integrated Haplotype Score (iHS), cross-population-extended haplotype homozygosity (XP-EHH), hapFLK, and FLK. A total of 404 top genomic regions were identified across all the four breeds, based on the four complementary analyses. Out of the 16 identified putative selection signature regions by the intersection of multiple-selective signal analyses, most of the putative regions were found to overlap significantly with the iHS and XP-EHH analyses on chromosomes 3, 4, 10, 15, 22, and 26. These regions were enriched with some genes involved in pathways associated directly or indirectly with environmental adaptation regulating immune responses (e.g., HYAL1 and HYAL3), milk production (e.g., LEPR and PDE4B), and adaptability (e.g., MST1 and PCK). The results revealed few intersect between breeds in genomic selection signature regions. In general, this did not present the typical classic selection signatures as predicted due to the complex nature of the traits. The results support that some various selection pressures (e.g., environmental challenges, artificial selection, and genome admixture challenges) have molded the genome of goat breeds in Kenya. Therefore, the research provides new knowledge on the conservation and utilization of these goat genetic resources in Kenya. In-depth research is needed to detect precise genes connected with adaptation and production in goat breeds in Kenya.

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“…Chen et al [ 39 ] reported that, in Porcine, there were strong relationships between intron 2 and exons 2 and 18 polymorphisms and reproductive features. As well as Alim et al [ 40 ] revealed that two SNPs detected within intron 3 and one SNP in exon 4 of LEPR gene and goats with heterozygous genotype AG at the loci g.104911A>G and g.105151A>G showed the highest prolificacy performance when compared with the other, homozygous genotypes. Sun et al [ 41 ] investigated that the majority of studied features in Luchuan and Large White pigs with AA genotype were greater than those with AB and BB genotypes ( P 0.05), according to the association analysis in the analyzed locus of LEPR gene exon 2 and in terms of litter size attributes, the AA genotype outperformed the AB and BB genotypes.…”

Section: Discussionmentioning

confidence: 90%

Genetic structure of some candidate genes of repeat breeder syndrome in Egyptian buffaloes

Mahrous

Kader

Abdelhafez

et al. 2022

Journal of Genetic Engineering and Biotechnology

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Background This study aimed to explore the association between polymorphisms in three genes: leptin (LEP), leptin receptor (LEPR), and BMP4, and incidence of repeat breeding in Egyptian buffaloes. Methods DNA was extracted from 160 female buffaloes, involving 108 fertile and 52 repeat breeders. Genotyping was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Sequence analysis and alignment were performed by employing NCBI/BLAST/blastn suite, to identify SNPs among different patterns and alleles. We utilized PredictSNP software to predict the non-synonymous SNPs influences on protein function. Moreover, the conservation score of the amino acids within the target proteins was computed by ConSurf server. Results The genotyping results showed that LEP and BMP4 genes were monomorphic (CC, GG) in all tested fertile and repeat breeder buffaloes. Leptin gene sequencing showed a non-synonymous C73T SNP, replacing R to C at position 25 within the leptin polypeptide (position 4 in the mature form; R4C) which is a neutral mutation, not affecting function or structure of LEP protein. For LEPR, one synonymous SNP (T102C) and two non-synonymous SNPs (A106G and C146A), triggering V967A and G954C replacements, respectively in LEPR protein. Moreover, they are neutral mutations. Sequencing results of BMP4 showed HinfI restriction site indicate fixed GG genotype (CC genotype in the anti-sense strand) in all sequenced samples. No SNPs were observed within the amplified region. Conclusion Genotyping and sequencing results of the surveyed three genes revealed that there is no association between these genes mutations and the incidence of repeat breeding in Egyptian buffaloes.

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Genetic effects of leptin receptor (LEPR) polymorphism on litter size in a Black Bengal goat population

Cited by 8 publications

References 0 publications

Selection Signatures Analysis Reveals Genes Associated with High-Altitude Adaptation in Tibetan Goats from Nagqu, Tibet

Selection Signatures Analysis Reveals Genes Associated with High-Altitude Adaptation in Tibetan Goats from Nagqu, Tibet

Selection Signature Analyses Revealed Genes Associated With Adaptation, Production, and Reproduction in Selected Goat Breeds in Kenya

Genetic structure of some candidate genes of repeat breeder syndrome in Egyptian buffaloes

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