Genetic effects of petroleum fuels: II. Analysis of chromosome loss and hyperploidy in peripheral lymphocytes of gasoline station attendants

Carere, A.; Antoccia, Antonio; Cimini, Daniela; Crebelli, Riccardo; Degrassi, Francesca; Leopardi, Paola; Marcon, Francesca; Sgura, Antonella; Tanzarella, Caterina; Zijno, Andrea

doi:10.1002/(sici)1098-2280(1998)32:2<130::aid-em8>3.0.co;2-#

Cited by 41 publications

(21 citation statements)

References 41 publications

(11 reference statements)

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“…SCEs were increased in the higher but not the lower exposure group in one preliminary study of workers exposed to organic solvents (Lalchev et al, 1997). While MN were increased in the higher exposure group of subjects in this same study (Lalchev et al, 1997), there was no change in MN frequency in another study that examined MN among gas station attendants using a FISH probe (Carere et al, 1998b). Finally, a recent study among 22 gas station attendants found a significant increase in MN, but not in SCEs, comparing with control subjects (Bukvic et al, 1998).…”

Section: Representative Studies In Workers Exposed To Petroleum Fumentioning

confidence: 44%

Section: B Comparison Of Data Obtained By Interphase and Metaphase Fishmentioning

confidence: 60%

“…No increase in hyperdiploidy of chromosomes 7, 11, 18, or X was detected either in interphase or metaphase cells (Carere et al, 1998b). Levels of polyploidy in exposed subjects were similar to those in controls when detected by either method (Carere et al, 1998b). However, it is difficult to draw conclusions about the relative sensitivities of interphase and metaphase FISH from this report because of the very small number of study subjects (six) and the fact that the benzene exposure was relatively low (0.1 ppm).…”

Section: B Comparison Of Data Obtained By Interphase and Metaphase Fishmentioning

confidence: 74%

“…Furthermore, individuals in these studies were subjected to a combination of chemical exposures. For these reasons, these studies are presented together in Table 7 (Betancourt et al, 1995;Carere et al, 1998b;Lalchev et al, 1997;Santos-Mello and Cavalcante, 1992;Silva and Santos-Mello, 1996;Bukvic et al 1998). Increased numerical CA were found in subjects working with auto paints, which used to contain benzene and other organic solvents (Silva and Santos-Mello, 1996).…”

Section: Representative Studies In Workers Exposed To Petroleum Fumentioning

confidence: 99%

“…As a result, recent scientific attention has begun to focus on the health effects of benzene at or near these lower levels. Interphase FISH has been employed to measure hyperdiploidy of chromosomes 7, 11, 18, and X in the lymphocytes of workers exposed to 0.1 ppm benzene (Carere et al, 1998b). No increased levels of hyperdiploidy were detected in any chromosome; in fact, slightly lower levels in comparison with controls were observed.…”

Section: A Aneuploidy Detected By Interphase Cytogeneticsmentioning

confidence: 99%

See 4 more Smart Citations

The Nature of Chromosomal Aberrations Detected in Humans Exposed to Benzene

Zhang¹,

Eastmond

Smith³

2002

Critical Reviews in Toxicology

151

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Section: Representative Studies In Workers Exposed To Petroleum Fumentioning

confidence: 44%

Section: B Comparison Of Data Obtained By Interphase and Metaphase Fishmentioning

confidence: 60%

Section: B Comparison Of Data Obtained By Interphase and Metaphase Fishmentioning

confidence: 74%

Section: Representative Studies In Workers Exposed To Petroleum Fumentioning

confidence: 99%

Section: A Aneuploidy Detected By Interphase Cytogeneticsmentioning

confidence: 99%

See 3 more Smart Citations

The Nature of Chromosomal Aberrations Detected in Humans Exposed to Benzene

Zhang¹,

Eastmond

Smith³

2002

Critical Reviews in Toxicology

151

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Genotoxicity biomonitoring in regions exposed to vehicle emissions using the comet assay and the micronucleus test in native rodent Ctenomys minutus

Heuser

Silva

Moriske

et al. 2002

Environ and Mol Mutagen

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Exposure to motor vehicle emissions represents an important concern for possible long-term health effects. The present report describes: 1) the application and verification of the alkaline comet assay in Ctenomys minutus to detect the possible genotoxicity of automobile emissions; 2) a comparison of the comet assay results with peripheral blood micronucleus (MN) assay results performed in the same animals; and 3) the identification of agents involved in the responses and in the seasonal variation of the effects. Ctenomys minutus (Octodontidae-Rodentia) were captured in two different fields from both sides of RS/030, a highway on the coastal plain of the Brazilian state of Rio Grande do Sul. Reference animals were obtained from a nearby field that was about 3 km distant from any road. By the end of this study, 123 rodents (73 females and 50 males) were live-trapped. Our results indicate that there was an increase in cells with DNA damage for C. minutus environmentally exposed to automobile emissions, as demonstrated by the alkaline comet assay, but there was no increase in micronucleated cells. The alkaline comet assay showed age and gender differences in the response. The comet assay results suggest that adult females are the principal population affected by air pollutants from vehicle emissions. Chemical data were also collected from areas exposed to automobile exhaust and these indicated that elevated levels of hydrocarbons, metals, and NO(2) were associated with the elevated levels of damaged cells observed in the wild rodent C. minutus. Our results agree with previous data on engine and fuel components, where weak increases in damage for native rodents exposed to emissions have been observed. Other larger, controlled studies are needed to better understand how the metabolism of C. minutus affects its response to emission exposure.

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Multipronged evaluation of genotoxicity in Indian petrol‐pump workers

Pandey

Bajpayee

Parmar

et al. 2008

Environ and Mol Mutagen

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Petrol (gasoline) contains a number of toxicants. This study used human biomonitoring to evaluate the genotoxic effects of exposure to benzene in petrol fumes in 100 Indian petrol-pump workers (PPWs) and an equal number of controls. The study was corroborated with in silico assessments of the Comet assay results from the human biomonitoring study. An in vitro study in human lymphocytes was also conducted to understand the genotoxicity of benzene and its metabolites. In a subset of the population studied, higher blood benzene levels were detected in the PPWs (n = 39; P < 0.01) than the controls (n = 18), and 100-250 ppb benzene was also detected in air samples from the petrol pumps. PPWs had higher levels of DNA damage than the controls (P < 0.01). In addition, the micronucleus assay was performed on lymphocytes from a subset of the subjects, and the micronucleus frequency for PPWs was significantly higher (n = 39; 14.79 +/- 3.92 per thousand) than the controls (n = 18; 7.54 +/- 3.00 per thousand). Human lymphocytes were treated in vitro with benzene and several of its metabolites and assayed for DNA damage with the Comet assay. Benzene and its metabolites produced significant (P < 0.05) levels of DNA damage at and above concentrations of 10 microM. The metabolite, p-benzoquinone, produced the greatest amount of DNA damage, followed by hydroquinone > benzene > catechol > 1,2,4,-benzenetriol > muconic acid. This study demonstrates that, using sensitive techniques, it is possible to detect human health risks at an early stage when intervention is possible. possible.

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Genetic effects of petroleum fuels: II. Analysis of chromosome loss and hyperploidy in peripheral lymphocytes of gasoline station attendants

Cited by 41 publications

References 41 publications

The Nature of Chromosomal Aberrations Detected in Humans Exposed to Benzene

The Nature of Chromosomal Aberrations Detected in Humans Exposed to Benzene

Genotoxicity biomonitoring in regions exposed to vehicle emissions using the comet assay and the micronucleus test in native rodent Ctenomys minutus

Multipronged evaluation of genotoxicity in Indian petrol‐pump workers

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