Genetic effects of the transcription factors‐sal‐like 1 and spalt‐like transcription factor 3 on egg production‐related traits in Chinese Dagu hens

Abstract

Transcription factors (TFs) encoded by SALL1 and SALL3 genes play central roles in the regulation of ovarian development in hens. The present study aimed to examine polymorphisms of these two genes in Chinese Dagu chickens, and to identify the effects of TFs on the laying performance. Among the population, two novel single-nucleotide polymorphisms (SNPs) were identified by single-strand conformation polymorphism (SSCP) in the amplicons of the candidate genes. The effect of the SNP (729C > A) in exon 2 of SALL1 gene on egg production at 43, 57, and 66 weeks and EW at 30 and 43 weeks were the most significant in the 360 samples (P < 0.05). Moreover, for the SNP 1014T > A (in exon 2 of SALL3), the TT genotype was significantly correlated with higher egg production and EW (P < 0.05). Furthermore, four combined genotypes were reconstructed based on the two SNPs. The combined genotype TATT was correlated with the highest egg production at 43-66 weeks and with higher EW at 30, 43 weeks (P < 0.05). The polymorphisms of the two TFs studied are potential molecular genetic markers for chicken breeding, which might help in understanding the genetic structure of laying performance and improving these traits directly by marker-assisted selection (MAS).

RNA-seq identifies differentially expressed genes involved in csal1 overexpression in granulosa cells of prehierarchical follicles in Chinese Dagu hens

“…The chicken is large and solid, with strong foraging ability, quality and quantity eggs, thick and solid eggshell, fresh and tender meat, and strong disease resistance. Our study had confirmed the correlation between csal1 and laying traits of Chinese Dagu chicken ( Zhu et al., 2018 ). However, the commercial production of Chinese Dagu chicken lacks systematic research on their laying performance.…”

Sensitivity and applications of the PCR Single-Strand Conformation Polymorphism method

“…PCR-SSCP has been used for several traits of animals such as wool characteristics [23], milk production traits [24], profile of sheep fatty acids [25], detection of Escherichia coli in the black swan [26], growth and carcass traits in lambs [27], reproductive traits in cattle [28], egg production traits in hens [29], body weight traits on chicken [30][31][32], gene study for fat-tailed and nonfat-tailed sheep [33], serotyping dichelobacter nodosus [34], mitochondrial diversity role in the productivity of quails [35], miR-9 gene affects litter size in goats [36], mutations in porcine [37], prolactin gene and association with egg production in ducks [38], wool fibre diameter in ewes [39], lactoferrin gene affects milk content in buffaloes [40], milk traits in yaks [41], association between MyoG gene and egg quality [42].…”

Exploring the Potential and Limitations of PCR-RFLP and PCR-SSCP for SNP Detection: A Review

“…57 However, PCR-SSCP was considered as a cornerstone in recent post-PCR genotyping studies on poultry, such as egg-production traits, body weight characteristics, and intramuscular fat content. [58][59][60] Because of its powerful ability to identify unknown SNP(s), Table 1. The Main Differences Between PCR-RFLP and PCR-SSCP Methods…”

“…[62][63][64] With respect to fish and amphibians, species identification and the detection of environmental samples and closely related organisms were recently conducted using PCR-RFLP. [56][57][58][59][60][61][62][63][64][65][66][67][68] The PCR-SSCP was used in the detection of parasite species. 69 The ease of the use of PCR-RFLP was demonstrated in its successful implementation in species identification and discrimination in algae, bacteria, and fungi.…”