Genetic Effects of X-Ray and Carbon Ion Irradiation in Head and Neck Carcinoma Cell Lines

Yamamoto, Nobuharu; Ikeda, Chihaya; Yakushiji, Takashi; Nomura, Takeshi; Katakura, Akira; Shibahara, Takahiko; Mizoe, Jun‐etsu

doi:10.2209/tdcpublication.48.177

Cited by 10 publications

(6 citation statements)

References 35 publications

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“…Their formation results from constitutional and/or inherited factors combined with environmental factors. Skin carcinoma are usually treated by surgical excision [2], radiotherapy [3][4][5], irradiation [6] or dynamic phototherapy [7]. However, these treatments are painful and invasive.…”

Section: Introductionmentioning

confidence: 99%

Transfection of Immortalized Keratinocytes by Low Toxic Poly(2-(Dimethylamino)Ethyl Methacrylate)-Based Polymers

Overstraeten-Schlögel

Ho-Shim

Tevel

et al. 2012

Journal of Biomaterials Science, Polymer Edition

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Skin carcinoma are among the most spread diagnosed tumours in the world. In this study, we investigated the transfection of immortalized keratinocytes, used as an in vitro model for skin carcinoma, using antisense technology and poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA)-based polymers, with original architecture and functionalities. We tested PDMAEMA polymers with different structures: linear, with two (DEA-PDMAEMA) or three (TEA-PDMAEMA) arms. The cytotoxicity of these polymers was assessed over a wide range of apparent M n (from 7600 to 64 600). At a N/P ratio of 7.38, cytotoxicity increases with the M n . Keratinocytes were transfected with a fluorescent oligonucleotide and then analyzed by flow cytometry. For the three architectures tested, the percentage of transfected cells and abundance of internalized oligonucleotide were closely related to the M n of the polymer. Confocal microscopy and FACS analyses showed a wide spread fine granular distribution of the oligonucleotide up to 3 days post-transfection. Then, we assessed the silencing efficiency of the polymers, targeting GFP in GFP expressing keratinocytes. The maximal silencing effect (±40%) was obtained using a DEA-PDMAEMA polymer (M n = 30 300). These results suggest that PDMAEMA-based polymers can be efficiently used to transfect immortalized keratinocytes and, thus, open new perspectives in the therapy of skin carcinoma.

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Section: Introductionmentioning

confidence: 99%

Transfection of Immortalized Keratinocytes by Low Toxic Poly(2-(Dimethylamino)Ethyl Methacrylate)-Based Polymers

Overstraeten-Schlögel

Ho-Shim

Tevel

et al. 2012

Journal of Biomaterials Science, Polymer Edition

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“…Examining X-ray and carbon ion induced effects on DNA in head carcinoma cells by using PCR of heterozygosity, Yamamoto et al observed that most of the X-ray induced DNA damage occurred in the target region on one of the homologous chromosomes, whereas carbon ions caused homo-deletion, i.e. deletion of the counterparts in both homologous chromosomes [51]. …”

Section: Introductionmentioning

confidence: 99%

Differences in Phosphorylated Histone H2AX Foci Formation and Removal of Cells Exposed to Low and High Linear Energy Transfer Radiation

Schmid

Zlobinskaya²,

Multhoff³

2012

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The use of particle ion beams in cancer radiotherapy has a long history. Today, beams of protons or heavy ions, predominantly carbon ions, can be accelerated to precisely calculated energies which can be accurately targeted to tumors. This particle therapy works by damaging the DNA of tissue cells, ultimately causing their death. Among the different types of DNA lesions, the formation of DNA double strand breaks is considered to be the most relevant of deleterious damages of ionizing radiation in cells. It is well-known that the extremely large localized energy deposition can lead to complex types of DNA double strand breaks. These effects can lead to cell death, mutations, genomic instability, or carcinogenesis. Complex double strand breaks can increase the probability of mis-rejoining by NHEJ. As a consequence differences in the repair kinetics following high and low LET irradiation qualities are attributed mainly to quantitative differences in their contributions of the fast and slow repair component. In general, there is a higher contribution of the slow component of DNA double strand repair after exposure to high LET radiation, which is thought to reflect the increased amount of complex DNA double strand breaks. These can be accurately measured by the γ-H2AX assay, because the number of phosphorylated H2AX foci correlates well with the number of double strand breaks induced by low or / and high LET radiation.

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“…In the previous study, it has been found that the survival fraction of cancer cells with carbon beam was lower than that of X-rays with same does, indicating that the carbon beam may have advantages over X-rays in radiotherapy 2,3,28. Although many studies have reported the changes of phenotype underlying different types of radiation, the molecular mechanism is still pending to be clarified to improve radiotherapy.…”

Section: Discussionmentioning

confidence: 99%

“…Although X-rays treatment is an effective modality for variety of human cancers, in certain cases it can provide poor results 1. Some studies report that heavy ion beam have obvious advantages over other radiotherapy mainly due to the spread out Bragg’s peaks (SOBP), which can cover tumors with biological equivalent dose distribution 2,3. Besides, heavy ion can also reduce oxygen enhancement ratio, decrease cell-cycle-dependent radiosensitivity, and induce more DNA double strand breaks that are not easily repaired 4–6.…”

Section: Introductionmentioning

confidence: 99%

Proteomic analysis of effects by x-rays and heavy ion in HeLa cells

Bing

Yang

Zhang

et al. 2014

Radiology and Oncology

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BackgroundCarbon ion therapy may be better against cancer than the effects of a photon beam. To investigate a biological advantage of carbon ion beam over X-rays, the radioresistant cell line HeLa cells were used. Radiation-induced changes in the biological processes were investigated post-irradiation at 1 h by a clinically relevant radiation dose (2 Gy X-ray and 2 Gy carbon beam). The differential expression proteins were collected for analysing biological effects.Materials and methods.The radioresistant cell line Hela cells were used. In our study, the stable isotope labelling with amino acids (SILAC) method coupled with 2D-LC-LTQ Orbitrap mass spectrometry was applied to identity and quantify the differentially expressed proteins after irradiation. The Western blotting experiment was used to validate the data.ResultsA total of 123 and 155 significantly changed proteins were evaluated with treatment of 2 Gy carbon and X-rays after radiation 1 h, respectively. These deregulated proteins were found to be mainly involved in several kinds of metabolism processes through Gene Ontology (GO) enrichment analysis. The two groups perform different response to different types of irradiation.ConclusionsThe radioresistance of the cancer cells treated with 2 Gy X-rays irradiation may be largely due to glycolysis enhancement, while the greater killing effect of 2 Gy carbon may be due to unchanged glycolysis and decreased amino acid metabolism.

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Genetic Effects of X-Ray and Carbon Ion Irradiation in Head and Neck Carcinoma Cell Lines

Cited by 10 publications

References 35 publications

Transfection of Immortalized Keratinocytes by Low Toxic Poly(2-(Dimethylamino)Ethyl Methacrylate)-Based Polymers

Transfection of Immortalized Keratinocytes by Low Toxic Poly(2-(Dimethylamino)Ethyl Methacrylate)-Based Polymers

Differences in Phosphorylated Histone H2AX Foci Formation and Removal of Cells Exposed to Low and High Linear Energy Transfer Radiation

Proteomic analysis of effects by x-rays and heavy ion in HeLa cells

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