Genetic Engineering and Transgenic Breeding

Ríos, Rodomiro Ortiz

doi:10.1007/978-3-319-20532-8_7

Cited by 4 publications

References 132 publications

(106 reference statements)

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Alternative Non‐AgrobacteriumBased Methods for Plant Transformation

Rathore

Mullins

2018

Annual Plant Reviews Online

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Plant breeding continues to play a crucial role in generating crops fit for purpose to tackle the increasing number of a biotic stressors that farmers face. While the majority of engineered varieties have been and continue to be generated through the use of Agrobacterium tumefaciens via Agrobacterium ‐mediated transformation (AMT), more recently, the potential of non‐ Agrobacterium systems is coming to the fore, specifically in regard to rhizobia such as Ensifer adhaerens , Ochrobactrum haywardense, Rhizobium etli , and the collective of strains termed Transbacter™. This article reviews the landscapes of non‐ Agrobacterium species from the perspective of crop improvement, highlighting the mechanisms they use to manipulate host genomes as the basis for their possible transinfection strategies. Recruiting enhanced versions of these non‐ Agrobacterium strains empowered with stealthy mechanisms to deliver DNA/proteins into plant cells provides the potential to make a positive contribution to enhancing existing breeding programmes.

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Alternative Non‐AgrobacteriumBased Methods for Plant Transformation

Rathore

Mullins

2018

Annual Plant Reviews Online

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Cisgenesis and intragenesis in microalgae: promising advancements towards sustainable metabolites production

Galarza

Delgado

Henríquez

2016

Appl Microbiol Biotechnol

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Microalgae are an economically important source of biomolecules and metabolites that can be exploited as feed, nutraceuticals and, potentially, as biofuels, among other biotechnological applications. Microalgae biotechnology involves both culture and metabolic pathways manipulation to obtain high-value products, such as omega-3 fatty acids and carotenoids. However, the introduction of genes and/or foreign regulatory sequences has caused public concern about the effect of genetically modified microalgae to achieve greater secondary metabolite accumulations. To placate these worries, we have focused on two cutting-edge concepts, cisgenesis and intragenesis in order to sustainably produce commercially relevant metabolites. This review provides updated background on current and future uses for microalgae molecular farming. We also discuss the development of genetic tools used in terrestrial plants to obtain genetically modified microalgae free of foreign DNA by means of (i) site-specific mutations, (ii) excision of selectable markers, (iii) zinc-finger nuclease and transcription activator-like effectors, and (iv) CRISPR/Cas9 systems. It is currently important to consider scientific debate not only from a technological standpoint but also in terms of conceptual, socioeconomic, ethical, and legal aspects.

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