Genetic engineering of bacteriophages: Key concepts, strategies, and applications

Hussain, Wajid; Yang, Xiaohan; Ullah, Mati; Wang, Huan; Aziz, Ayesha; Xu, Fang; Asif, Muhammad; Ullah, Muhammad Wajid; Wang, Shenqi

doi:10.1016/j.biotechadv.2023.108116

Cited by 23 publications

(12 citation statements)

References 203 publications

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“…These nanofibers aid in enhancing neural stem cell activity and stimulating vascular generation at stroke sites, potentially enhancing stroke therapies. Genetic engineering techniques are rapidly advancing bacteriophage research ( 125 ). The distinctive architecture of bacteriophages enables integration of exogenous peptides onto their coat proteins, endowing these peptides with targeting attributes that bolster disease treatment ( 16 ).…”

Section: Discussionmentioning

confidence: 99%

Harnessing filamentous phages for enhanced stroke recovery

Li,

Yang,

Kong

et al. 2024

Front. Immunol.

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Stroke poses a critical global health challenge, leading to substantial morbidity and mortality. Existing treatments often miss vital timeframes and encounter limitations due to adverse effects, prompting the pursuit of innovative approaches to restore compromised brain function. This review explores the potential of filamentous phages in enhancing stroke recovery. Initially antimicrobial-centric, bacteriophage therapy has evolved into a regenerative solution. We explore the diverse role of filamentous phages in post-stroke neurological restoration, emphasizing their ability to integrate peptides into phage coat proteins, thereby facilitating recovery. Experimental evidence supports their efficacy in alleviating post-stroke complications, immune modulation, and tissue regeneration. However, rigorous clinical validation is essential to address challenges like dosing and administration routes. Additionally, genetic modification enhances their potential as injectable biomaterials for complex brain tissue issues. This review emphasizes innovative strategies and the capacity of filamentous phages to contribute to enhanced stroke recovery, as opposed to serving as standalone treatment, particularly in addressing stroke-induced brain tissue damage.

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Section: Discussionmentioning

confidence: 99%

Harnessing filamentous phages for enhanced stroke recovery

Li,

Yang,

Kong

et al. 2024

Front. Immunol.

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“…Phages are one of the most ubiquitous organisms on earth, thriving in diverse habitats alongside specific host bacteria [ 172 , 173 ]. According to the life cycle of infection, phages can be divided into two types: lytic phages and lysogenic phages.…”

Section: Novel Approaches and Strategies For Promoting Biofilm Penetr...mentioning

confidence: 99%

Contemporary strategies and approaches for characterizing composition and enhancing biofilm penetration targeting bacterial extracellular polymeric substances

Lu,

Zhao,

et al. 2024

Journal of Pharmaceutical Analysis

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“…Genetically modified phages are also used in the development of vaccines and in tissue engineering. For further information, the reader is referred to the review by Hussain et al [ 189 ]. The introduction of rpsL and gyrA in lysogenic phages increased the sensitivity of pathogens to streptomycin and nalidixic acid, respectively [ 190 ].…”

Section: Genomic Engineering Of Phagesmentioning

confidence: 99%

“…The introduction of rpsL and gyrA in lysogenic phages increased the sensitivity of pathogens to streptomycin and nalidixic acid, respectively [ 190 ]. This approach may be used in the treatment of methicillin-resistant S. aureus , as shown in the treatment of skin infections [ 191 ] and bacterial infections associated with wounds [ 189 , 192 ], burns [ 119 , 190 , 191 , 193 ], and diabetic leg and foot ulcers [ 192 , 194 , 195 , 196 ]. Phages have also been used in the treatment of wound sepsis caused by multidrug-resistant P. aeruginosa [ 197 ].…”

Section: Genomic Engineering Of Phagesmentioning

confidence: 99%

Bacteriophage–Host Interactions and the Therapeutic Potential of Bacteriophages

Dicks,

Vermeulen

2024

Viruses

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Healthcare faces a major problem with the increased emergence of antimicrobial resistance due to over-prescribing antibiotics. Bacteriophages may provide a solution to the treatment of bacterial infections given their specificity. Enzymes such as endolysins, exolysins, endopeptidases, endosialidases, and depolymerases produced by phages interact with bacterial surfaces, cell wall components, and exopolysaccharides, and may even destroy biofilms. Enzymatic cleavage of the host cell envelope components exposes specific receptors required for phage adhesion. Gram-positive bacteria are susceptible to phage infiltration through their peptidoglycan, cell wall teichoic acid (WTA), lipoteichoic acids (LTAs), and flagella. In Gram-negative bacteria, lipopolysaccharides (LPSs), pili, and capsules serve as targets. Defense mechanisms used by bacteria differ and include physical barriers (e.g., capsules) or endogenous mechanisms such as clustered regularly interspaced palindromic repeat (CRISPR)-associated protein (Cas) systems. Phage proteins stimulate immune responses against specific pathogens and improve antibiotic susceptibility. This review discusses the attachment of phages to bacterial cells, the penetration of bacterial cells, the use of phages in the treatment of bacterial infections, and the limitations of phage therapy. The therapeutic potential of phage-derived proteins and the impact that genomically engineered phages may have in the treatment of infections are summarized.

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Genetic engineering of bacteriophages: Key concepts, strategies, and applications

Cited by 23 publications

References 203 publications

Harnessing filamentous phages for enhanced stroke recovery

Harnessing filamentous phages for enhanced stroke recovery

Contemporary strategies and approaches for characterizing composition and enhancing biofilm penetration targeting bacterial extracellular polymeric substances

Bacteriophage–Host Interactions and the Therapeutic Potential of Bacteriophages

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