Genetic engineering of crops for insect resistance: An overview

Talakayala, Ashwini; Katta, Sumalatha; Mallikarjuna, G. B.

doi:10.1007/s12038-020-00081-y

Cited by 24 publications

(8 citation statements)

References 62 publications

(10 reference statements)

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“…Alpha-amylase (α-Amylase) is an enzyme that can hydrolyze α-D- (1,4)-glycosidic bonds in starch or polysaccharides. Starch and polysaccharides must be hydrolyzed into disaccharides and oligosaccharides which are then hydrolyzed by the α-glycosidase enzyme to monosaccharide molecules that are absorbed into the hepatic portal vena via the small intestine .…”

Section: Introductionmentioning

confidence: 99%

“…Almost one-fourth of global crops are destroyed by insects annually . [4] Some larvae rely on the starch in crops, against which amylase inhibitors in plants provide some defense . [5] Research [6] has shown that the expression profiles of important DNA repair genes in bone marrow stem cells are negatively affected by the genotoxicity of pesticides.…”

Section: Introductionmentioning

confidence: 99%

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Proteinaceous α-amylase inhibitors: purification, detection methods, types and mechanisms

Zhou

Zhang

et al. 2021

International Journal of Food Properties

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α-Amylase is abundant in plants and animals. α-Amylase inhibitors can reduce endogenous α-amylase activity, playing an essential role in agricultural pest control, and preventing and treating human disease. In the agricultural field, α-Amylase inhibitors can restrict pest that relies on the starch of crops. Acarbose is an α-amylase inhibitor used to treat diabetes. Some αamylase inhibitors are represented by antinutritional factors, while others are proteinaceous. Depending on their structures and sources, researchers have divided them into seven types: The knottin-like type, the γ-thionin-like type, the cereal type, the Kunitz type, the thaumatin-like type, and the lectin-like type. This paper introduces the methods for separating, purifying, and detecting proteinaceous α-amylase inhibitors while examining the structure and inhibition mechanism of several proteinaceous α-amylase inhibitors. Finally, it explores the potential applications of α-amylase inhibitors.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Proteinaceous α-amylase inhibitors: purification, detection methods, types and mechanisms

Zhou

Zhang

et al. 2021

International Journal of Food Properties

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“…A wide range of studies have shown the feasibility of transgenic crops protecting plants against pests and pathogens ( Baum et al, 2007 ; Mao et al, 2011 ; Pitino et al, 2011 ; Zha et al, 2011 ; Zhang et al, 2015 ; Malik et al, 2016 ; Yu et al, 2016 ; Khalid et al, 2017 ; Li et al, 2017 ). Transgenic crop protection has also been reviewed in a number of publications ( Talakayala et al, 2020 ; Chung et al, 2021 ; Kumari et al, 2022 ). The main advantage associated with in planta dsRNA synthesis (endogenous dsRNA production) in comparison to alternative production systems, is the elimination of downstream processing, with no purification, formulation and/or delivery of the dsRNA or material containing the dsRNA required, thus reducing labour for agricultural workers and consequently reducing costs to the consumer ( Zotti and Smagghe, 2015 ).…”

Section: Strategies For Production Of Dsrna For Crop Protectionmentioning

confidence: 99%

Strategies for the production of dsRNA biocontrols as alternatives to chemical pesticides

Hough

Howard

Brown

et al. 2022

Front. Bioeng. Biotechnol.

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Current crop pest control strategies rely on insecticidal and fungicidal sprays, plant genetic resistance, transgenes and agricultural practices. However, many insects, plant viruses, and fungi have no current means of control or have developed resistance against traditional pesticides. dsRNA is emerging as a novel sustainable method of plant protection as an alternative to traditional chemical pesticides. The successful commercialisation of dsRNA based biocontrols for effective pest management strategies requires the economical production of large quantities of dsRNA combined with suitable delivery methods to ensure RNAi efficacy against the target pest. A number of methods exist for the production and delivery of dsRNA based biocontrols and here we review alternative methods currently employed and emerging new approaches for their production. Additionally, we highlight potential challenges that will need to be addressed prior to widespread adoption of dsRNA biocontrols as novel sustainable alternatives to traditional chemical pesticides.

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“…The different biotechnological approaches include gene transformation, genome editing, RNA interference, marker-assisted selection, anther culture, embryo culture, protoplast fusion, somaclonal variations etc. ( Talakayala et al, 2020 ). These approaches are described in detail below.…”

Section: Biotechnological Approaches In Insect Pest Managementmentioning

confidence: 99%

Biotechnological Approaches for Host Plant Resistance to Insect Pests

et al. 2022

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Annually, the cost of insect pest control in agriculture crosses billions of dollars around the world. Until recently, broad-spectrum synthetic pesticides were considered as the most effective means of pest control in agriculture. However, over the years, the overreliance on pesticides has caused adverse effects on beneficial insects, human health and the environment, and has led to the development of pesticide resistant insects. There is a critical need for the development of alternative pest management strategies aiming for minimum use of pesticides and conservation of natural enemies for maintaining the ecological balance of the environment. Host plant resistance plays a vital role in integrated pest management but the development of insect-resistant varieties through conventional ways of host plant resistance takes time, and is challenging as it involves many quantitative traits positioned at various loci. Biotechnological approaches such as gene editing, gene transformation, marker-assisted selection etc. in this direction have recently opened up a new era of insect control options. These could contribute towards about exploring a much wider array of novel insecticidal genes that would otherwise be beyond the scope of conventional breeding. Biotechnological interventions can alter the gene expression level and pattern as well as the development of transgenic varieties with insecticidal genes and can improve pest management by providing access to novel molecules. This review will discuss the emerging biotechnological tools available to develop insect-resistant engineered crop genotypes with a better ability to resist the attack of insect pests.

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Genetic engineering of crops for insect resistance: An overview

Cited by 24 publications

References 62 publications

Proteinaceous α-amylase inhibitors: purification, detection methods, types and mechanisms

Proteinaceous α-amylase inhibitors: purification, detection methods, types and mechanisms

Strategies for the production of dsRNA biocontrols as alternatives to chemical pesticides

Biotechnological Approaches for Host Plant Resistance to Insect Pests

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