2015
DOI: 10.1073/pnas.1424877112
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Genetic engineering of hematopoietic stem cells to generate invariant natural killer T cells

Abstract: Invariant natural killer T (iNKT) cells comprise a small population of αβ T lymphocytes. They bridge the innate and adaptive immune systems and mediate strong and rapid responses to many diseases, including cancer, infections, allergies, and autoimmunity. However, the study of iNKT cell biology and the therapeutic applications of these cells are greatly limited by their small numbers in vivo (∼0.01-1% in mouse and human blood). Here, we report a new method to generate large numbers of iNKT cells in mice throug… Show more

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Cited by 34 publications
(39 citation statements)
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“…Invitrogen eBioscience Fixable Viability Dye eFluor 506 (catalog 65-0866) was purchased from Thermo Fisher Scientific. Cells were stained with Fixable Viability Dye first, followed by Fc blocking and surface marker staining, using a standard, previously described procedure (61 and 400 ng PTX. On day 1, a second dose of PTX (400 ng per mouse) was injected.…”
Section: Discussionmentioning
confidence: 99%
“…Invitrogen eBioscience Fixable Viability Dye eFluor 506 (catalog 65-0866) was purchased from Thermo Fisher Scientific. Cells were stained with Fixable Viability Dye first, followed by Fc blocking and surface marker staining, using a standard, previously described procedure (61 and 400 ng PTX. On day 1, a second dose of PTX (400 ng per mouse) was injected.…”
Section: Discussionmentioning
confidence: 99%
“…In a similar approach, it is possible that engineering T cells to express modified TCRs, or CARs recognizing HIV antigens, could provide control of HIV or eliminate infected cells. Other immune effector cells are also being developed as candidates for these methods 81 .…”
Section: Cell and Gene Therapymentioning
confidence: 99%
“…Using these clonal cells, they were able to protect mice from tumor metastasis. Per Dr. Yang, her method has opened a door for a larger supply of engineered iNKT cells and iNKT cell-based immunotherapy [32]. …”
Section: Immunotherapymentioning
confidence: 99%