Genetic Engineering of <i>Nicotiana tabacum</i> for Reduced Nornicotine Content

Gavilano, Lily; Coleman, Nicholas P.; Burnley, Leigh-Emma; Bowman, Melissa; Kalengamaliro, Newton E.; Hayes, A. J.; Bush, Lowell P.; Siminszky, Balazs

doi:10.1021/jf0610458

Cited by 61 publications

(52 citation statements)

References 28 publications

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“…Our results further reinforce that hypothesis. Thus, removal of nornicotine from tobacco could be a potential strategy to reduce the levels of NNN in tobacco products (Gavilano et al, 2006).…”

Section: Resultsmentioning

confidence: 99%

Levels of (S)-N'-Nitrosonornicotine in U.S. Tobacco Products

Stepanov¹,

Yershova²,

Carmella³

et al. 2012

Nicotine & Tobacco Research

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Section: Resultsmentioning

confidence: 99%

Levels of (S)-N'-Nitrosonornicotine in U.S. Tobacco Products

Stepanov¹,

Yershova²,

Carmella³

et al. 2012

Nicotine & Tobacco Research

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“…Numerous studies have indicated that IR constructs of transgenes can effectively induce RNA silencing to trigger knockout of specific gene expressions (Ali et al, 2010;Allen et al, 2004;Gavilano et al, 2006;Johansen and Carrington, 2001;Kusaba et al, 2003;Liu et al, 2002;Meli et al, 2010;Pandolfini et al, 2003;Segal et al, 2003;Smith et al, 2000;Xiong et al, 2005). In addition, a number of studies showed that IR constructs of transgenes containing virus-derived sequences transformed to plants can effectively protect plants from challenging viruses (Abhary et al 2006;Bucher et al 2006;Chen et al, 2004;Di Nicola-Negri et al 2005;Hammond et al 2006;Lennefors et al 2006;Pooggin et al 2003;Simón-Mateo and García, 2011;Tenllado et al 2003;Tenllado et al, 2004;Vanitharani et al, 2003;Wang et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

Transient Expression of Homologous Hairpin RNA Interferes with Broad bean wilt virus 2 Infection in Nicotiana benthamiana

Yoon¹,

Ryu²,

Choi³

et al. 2012

Research in Plant Disease

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Broad bean wilt virus 2 (BBWV2), genus Fabavirus, subfamily Comovirinae, family Secoviridae, causes damage in many economically important horticultural and ornamental crops. Sequence alignments showed several conserved sequences in 5' non-coding regions (5' NCRs) of RNA 1 and RNA 2 in all BBWV2 strains characterized so far. Based on this observation, we generated a hpRNA construct (pIR-BBWV2) harboring an inverted repeat containing a 210 bp cDNA fragment homologous to 5' NCR portion of BBWV2 RNA 1 to investigate the silencing potential for its ability to interfere with a rapidly replicating BBWV2. Agrobacteriummediated transient expression of the IR-BBWV2 had a detrimental effect on BBWV2 infection, showing no distinct symptoms in non-inoculated leaves of the agroinfiltrated Nicotiana benthamiana plants. BBWV2 genomic RNAs were not detected by RT-PCR from tissues of both the inoculated leaves and upper leaves of the agroinfiltrated plants. Accumulation of virus-derived small interfering RNAs was detected in the inoculated leaf tissues of N. benthamiana plants elicited by transient expression of IR-BBWV2 indicating that RNA silencing is responsible for the resistance to BBWV2.

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“…The inhibition of nicotine demethylase activity is an effective mean of decreasing significantly the level of defined carcinogen present in tobacco products. Gavilano et al (2006) used RNAi for silencing the N-demethylase (CYP82E4) gene for suppressing the conversion of nicotine to nor-nicotine in tobacco (Nicotiana tabacum). Further, Lewis et al (2008) developed RNAi-silenced nicotine demethylase transgenic lines of burley tobacco line (DH98-325-5) exhibiting sixfold decrease in nor-nicotine content.…”

Section: Allergen and Toxin Eliminationmentioning

confidence: 99%