Genetic Engineering of Russian Wheat Genotypes for Abiotic Stress Resistance

Miroshnichenko, Dmitry; Filippov, M. V.; Бабаков, А. В.; Dolgov, Sergey

doi:10.1007/1-4020-5497-1_87

Cited by 2 publications

(3 citation statements)

References 8 publications

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“…Homozygous T 1 sub-lines were identified by spraying of T 2 seedlings with the herbicide Basta and through analysis of segregation using gfp fluorescence. Further seeds were propagated in the greenhouse and field plots (Miroshnichenko et al 2007a) to obtain sufficient homozygous T 3 -T 5 seed material for further experiments.…”

Section: Plant Materialsmentioning

confidence: 99%

“…GM wheat plants expressing bar gene successfully survive the spraying with a high herbicide concentration up to 100 mg PPT/L (Cannell et al 1999;Barro et al 2002;Becker et al 2004). Generally the same level of herbicide tolerance exists between homozygous and heterozygous T 1 -T n transgenic wheat progenies (Miroshnichenko et al 2007a;Foetzki et al 2012).…”

Section: Introductionmentioning

confidence: 98%

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Assessment of the pollen-mediated transgene flow from the plants of herbicide resistant wheat to conventional wheat (Triticum aestivum L.)

2016

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Three-year field assessment of gene flow from the genetically modified herbicide-resistant spring wheat variety 'Andros' to its non-transgenic counterpart has been conducted. A circular field trial design where the wheat plants containing the bar and gfp genes were planted in a central plot, while the recipient non-transgenic plants were grown in eight compass sectors at a distance of 1-5 m from the pollen source, has been developed. Gene flow was analyzed by testing the glufosinate-based herbicide resistance in seedling progeny. The phenotypic and molecular examination of more than 712,000 germinated seeds allowed us to draw a conclusion that the pollenmediated transgene flow might occur at a low frequency (\0.8 %) even if the nontransgenic wheat was located in the proximity to its transgenic counterpart. The strong asymmetric distribution of gene flow and the maximum outcrossing rate were detected in compass sectors following the direction of the dominant wind. The gene flow rate averaged over all wind direction varied in different years from 0.134 to 0.416 %. The dramatic reduction in transgene flow frequencies was observed while increasing the distance between transgenic and non-transgenic plots. The rise of average gene flow due to significant increase of the transgene pollen area was not confirmed statistically. These results predict that the pollen-mediated transgene flow in spring wheat can be maintained at negligible levels while the existence of a short isolation distance in order to prevent contamination of adjacent non-GM wheat.

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Section: Plant Materialsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

Assessment of the pollen-mediated transgene flow from the plants of herbicide resistant wheat to conventional wheat (Triticum aestivum L.)

2016

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“…Though there is a great demand for wheat varieties with resistance to diseases, genes conferred with these traits are limited, as they are mostly quantitative traits. Nonetheless, the direct introduction of a small number of genes by genetic engineering offers a convenient and rapid approach for the improvement of stress tolerance [6].…”

Section: Wheat Genetic Transformation As Efficient Tools To Fight Agamentioning

confidence: 99%

Wheat Genetic Transformation as Efficient Tools to Fight against Fungal Diseases

Ekom¹,

Benchekroun²,

Iraqi³

et al. 2015

JAST-A

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Abstract:Wheat ranks first among cereal crops cultivated in the world. In its production, diseases like powdery mildew, fusarium head blight and rusts caused by fungal pathogens represent a major problem. They produce different symptoms that cause severe crop damage by infecting the spikes, leaves, roots, stems and grains. They are causing losses both by reducing the quantity of the harvested crop and the quality of the product. Quality problems of the harvested product can be due to shrivelled seed, which are frequently found as a consequence of the infection by leaf pathogens, such as mildews, rusts and Septoria. Fusarium head blight is the major culprit for mycotoxin contamination from the harvested grain, causing economic losses and in the worst casing human and animal health problems. In severe epidemics, all these fungal diseases can significantly reduce yield. Resistance to fungi is beneficial not only from a commercial point of view (yield), but also because of the reduced levels of mycotoxins. The integration of transgenic approaches offers a potential chemical-free and environment-friendly solution for controlling fungal pathogens. This is an essential asset for wheat world food security.

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Genetic Engineering of Russian Wheat Genotypes for Abiotic Stress Resistance

Cited by 2 publications

References 8 publications

Assessment of the pollen-mediated transgene flow from the plants of herbicide resistant wheat to conventional wheat (Triticum aestivum L.)

Assessment of the pollen-mediated transgene flow from the plants of herbicide resistant wheat to conventional wheat (Triticum aestivum L.)

Wheat Genetic Transformation as Efficient Tools to Fight against Fungal Diseases

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