Genetic Environment of the
            <i>lnu</i>
            (B) Gene in a Streptococcus agalactiae Clinical Isolate

Montilla, A.; Zavala, Agustín; Cáceres, R. Cáceres; Cittadini, R.; Vay, Carlos; Gutkind, Gabriel; Famiglietti, Ángela; Bonofiglio, Laura; Mollerach, Marta

doi:10.1128/aac.02630-14

Cited by 30 publications

(29 citation statements)

References 12 publications

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“…7–12,37 Among our isolates, lnu (B) is invariably present in combination with lsa (E) on the same transposable element, as previously described in S. aureus 15 and GBS, 33 and in association with cross-resistance to clindamycin, virginiamycin M1 and tiamulin (LSAP). The MIC values against virginiamycin M1 and tiamulin for these isolates positive for both lnu (B) and lsa (E) were up to 4-fold and 16-fold higher, respectively.…”

Section: Discussionsupporting

confidence: 59%

“…Among these isolates, lnu (B) and lsa (E) were carried on a 75.5 kb mobile element, containing genes from two other elements: one described in a GBS isolate (SGB76) 33 and one described in a Streptococcus suis isolate (SC070731); 34 this element was invariably integrated into the chromosome at rum (A) [23S rRNA(uracil-5-)methyltransferase], in the same position as previously described for van (G)-containing elements in GBS and Streptococcus anginosus 35 (Figure 2a). …”

Section: Resultsmentioning

confidence: 99%

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Cross-resistance to lincosamides, streptogramins A and pleuromutilins in Streptococcus agalactiae isolates from the USA

Hawkins

Law

Metcalf

et al. 2017

Journal of Antimicrobial Chemotherapy

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Background Streptococcus agalactiae (Group B Streptococcus, GBS) is a leading cause of meningitis, sepsis and pneumonia in neonates in the United States. GBS also causes invasive disease in older infants, pregnant women, children and young adults with underlying medical conditions, and older adults. Resistance to lincosamides in the absence of erythromycin resistance is rare in GBS, but has been previously reported in clinical isolates, both on its own or in combination with resistance to streptogramins A and pleuromutilins (L/LSA/LSAP phenotypes). Objectives To retrospectively screen the Active Bacterial Core surveillance (ABCs) GBS isolate collection for these phenotypes in order to identify the causal genetic determinants and determine whether their frequency is increasing. Methods Based on MIC data, 65 (0.31%) isolates susceptible to erythromycin (MIC ≤0.25 mg/L) and non-susceptible to clindamycin (MIC ≥0.5 mg/L) were identified among 21186 GBS isolates. Genomic DNA was extracted and WGS was performed. The presence of 10 genes previously associated with LSA resistance was investigated by read mapping. Results Forty-nine (75%) isolates carried the lsa(C) gene and expressed the LSAP phenotype, and 12 (18%) carried both the lnu(B) and lsa(E) genes and expressed the LSAP phenotype. The four remaining isolates were negative for all determinants investigated. Conclusions While the overall observed frequency of these phenotypes among our GBS isolates was quite low (0.31%), this frequency has increased in recent years. To the best of our knowledge, this is the first time the LSAP phenotype has been reported among GBS isolates from the USA.

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Section: Discussionsupporting

confidence: 59%

Section: Resultsmentioning

confidence: 99%

Cross-resistance to lincosamides, streptogramins A and pleuromutilins in Streptococcus agalactiae isolates from the USA

Hawkins

Law

Metcalf

et al. 2017

Journal of Antimicrobial Chemotherapy

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“…Members of the RepA_N and Inc18 families are often enriched in insertion sequences, mainly IS257, IS256, IS1216, ISL3, and IS431, that facilitate co-integration, rearrangements, and deletions among elements of Staphylococcus, Enterococcus, LAB, and Clostridium of different origins (6,7,28,(90)(91)(92)(93)(94)(95). Such recombination events seem to have facilitated the origin of the great mosaicism of MDR plasmids that often carry more than one RIP, lack transfer and maintenance modules, and eventually carry more than one REL ( Fig.…”

Section: Theta-replicating Plasmidsmentioning

confidence: 99%

The Plasmidome of Firmicutes: Impact on the Emergence and the Spread of Resistance to Antimicrobials

et al. 2015

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The phylum Firmicutes is one of the most abundant groups of prokaryotes in the microbiota of humans and animals and includes genera of outstanding relevance in biomedicine, health care, and industry. Antimicrobial drug resistance is now considered a global health security challenge of the 21st century, and this heterogeneous group of microorganisms represents a significant part of this public health issue.

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“…Recently, it has been detected as part of multiresistance gene clusters on plasmids or in the chromosomal DNA of S. aureus, Staphylococcus hyicus, E. faecium, E. faecalis, Streptococcus agalactiae, and Erysipelothrix rhusiopathiae of human and animal origin (Fig. 4) (Lozano et al 2012a;Li et al , 2014bMontilla et al 2014;Silva et al 2014;Wendlandt et al 2014Wendlandt et al , 2015aZhang et al 2015a).…”

Section: Enzymatic Inactivation Of Lincosamidesmentioning

confidence: 99%

Lincosamides, Streptogramins, Phenicols, and Pleuromutilins: Mode of Action and Mechanisms of Resistance

Schwarz

Shen

Kadlec

et al. 2016

Cold Spring Harb Perspect Med

108

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Lincosamides, streptogramins, phenicols, and pleuromutilins (LSPPs) represent four structurally different classes of antimicrobial agents that inhibit bacterial protein synthesis by binding to particular sites on the 50S ribosomal subunit of the ribosomes. Members of all four classes are used for different purposes in human and veterinary medicine in various countries worldwide. Bacteria have developed ways and means to escape the inhibitory effects of LSPP antimicrobial agents by enzymatic inactivation, active export, or modification of the target sites of the agents. This review provides a comprehensive overview of the mode of action of LSPP antimicrobial agents as well as of the mutations and resistance genes known to confer resistance to these agents in various bacteria of human and animal origin.

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Genetic Environment of the lnu (B) Gene in a Streptococcus agalactiae Clinical Isolate

Cited by 30 publications

References 12 publications

Cross-resistance to lincosamides, streptogramins A and pleuromutilins in Streptococcus agalactiae isolates from the USA

Cross-resistance to lincosamides, streptogramins A and pleuromutilins in Streptococcus agalactiae isolates from the USA

The Plasmidome of Firmicutes: Impact on the Emergence and the Spread of Resistance to Antimicrobials

Lincosamides, Streptogramins, Phenicols, and Pleuromutilins: Mode of Action and Mechanisms of Resistance

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