Genetic features of livestock-associated Staphylococcus aureus ST9 isolates from Chinese pigs that carry the lsa(E) gene for quinupristin/dalfopristin resistance

Abstract

Whole-genome sequencing (WGS) was used to investigate the genetic features of the recently identified lsa(E) gene in porcine S. aureus ST9 isolates. Three quinupristin/dalfopristin-resistant isolates harboring the lsa(E) gene (two MRSA and one MSSA) were sequenced. Phylogenetic analysis of 184S. aureus genomes showed that ST9 porcine isolates belong to a distinct sequence cluster. Further analysis showed that all isolates were deficient in the recently described type IV restriction-modification system and SCCmec type XII was identified in the two MRSA isolates, which included a rare class C2 mec gene complex. A 24kb ΨSCC fragment was found in the MRSA and MSSA isolates sharing 99% nucleotide sequence homology with the ΨSCCJCSC6690 (O-2) element of a ST9 MRSA isolate from Thailand (accession number AB705453). Comparison of these ST9 isolates with 181 publically available S. aureus genomes identified 24 genes present in all (100%) ST9 isolates, that were absent from the most closely related human isolate. Our analysis suggests that the sequenced quinupristin/dalfopristin-resistant ST9 lineage represent a reservoir of mobile genetic elements associated with resistance and virulence features.

Molecular Characteristics of Staphylococcus aureus Causing Bovine Mastitis between 2014 and 2015

“…The resulting spa type was assigned by submitting the data to the S. aureus spa type database (http://www.spaserver.ridom.de). Staphylococcal cassette chromosome mec (SCC mec ) types were determined by means of two multiplex PCRs (M-PCRs) designed for the detection of the mec -complex and the ccr -complex (Kondo et al, 2007; Yan et al, 2016). Specific primers, as previously described (Wu et al, 2015) was used in isolates with SCC mec types that could not be determined using the SCC mec typing strategy as previously described (Kondo et al, 2007).…”

Recombination-Mediated Host Adaptation by Avian Staphylococcus aureus

“…2010, 2015; Weinert et al. 2012), including acquisition of genes that contribute to transmission, colonization, and virulence (Wiedenbeck and Cohan 2011; Yan et al. 2016).…”

Livestock-associated Methicillin-resistant Staphylococcus aureus as a New Impendence for Public Health

“…Стафилококки группы LA-MRSA CC398 устойчивы к макролидам, широко применяющимся в ветеринарной практике для лечения и профилактики инфекционных заболеваний, в том числе к эритромицину и клиндамицину [12,40]. Устойчивость определяется генами ermC, ermA и ermB, продуктами которых являются метилазы, осуществляющие модификацию 50S субъединиц рибосом [41].…”

“…Также распространена устойчивость к стрептограмину [42]. Устойчивость к стрепрограмину A у стафилококков может определяться ацетилтрансферазой Vat, ABCтранспортёрами Vga или Lsa, а также активностью метилтрансферазы Cfr [40].…”

“…Для изолятов стафилококка группы LA-MRSA CC398 характерна множественная резистентность, выражающаяся в сочетанной устойчивости к противомикробным препаратам 3 и более групп [7,40], что затрудняет лечение ассоциированных с ним заболеваний. В то же время только единичные изоляты LA-MRSA CC398 зоонозного происхождения характеризуются устойчивостью к фузидиновой кислоте, рифампицину и линезолиду.…”