Genetic Fingerprinting of Pseudomonas aeruginosa Involved in Nosocomial Infection as Revealed by RAPD-PCR Markers

Akanji, B.O.; Ajele, Joshua O.; Onasanya, A.; Oyelakin, Oladele

doi:10.3923/biotech.2011.70.77

Cited by 14 publications

(11 citation statements)

References 13 publications

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“…The prevalence of P. aeruginosa isolates in this study was lower than ours, which represents the difference of bacteria involved in infections in various parts of the world. In a study, 89 cases of P. aeruginosa were isolated from different clinical specimens, which the highest prevalence was found in urine samples by 51.5% and were resistant to Ceftazidime (56%) and imipenem (24.7%), representing the annual increase (Akanji et al,2011).…”

Section: Resultsmentioning

confidence: 99%

Comparison of genetic and drug resistance patterns in Pseudomonas aeruginosa isolated from the burn wound patients using primers 272 and 277

Mahmmudi¹,

Gorzin²,

Emami³

2016

Biosci. Biotech. Res. Comm

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Pseudomonas aeruginosa is one the main causes of opportunistic infections, especially in subjects with weakened immune systems such as patients with burns. Carbapenems such as imipenem (IMP) and meropenem (MEM) are among the most important antimicrobial drugs which are used to treat P. aeruginosa isolates resistant to penicillin and aminoglycosides. Given that identify the source of contamination and the resulting pattern of isolates resistant transmission in health centers play an important role in the control of hospital infections, the aim of this study was to determine antibiotic susceptibility and genetic patterns of P. aeruginosa bacteria isolated from patients admitted to Qutb al-Din Shirazi hospital. Of 200 samples collected from hospitalized burn patients at Qutb al-Din Shirazi Hospital Shiraz, Iran, 50 (25%) isolates were confi rmed as P. aeruginosa using standard biochemical tests. Antibiotic susceptibility of the isolates was examined through 11 antibiotics recommended by CLSI using the Kirby-Bauer method. RAPD-PCR technique with short primers of 272 and 277 were used to evaluate the genetic relationship among isolates. Of the antibiotics used, the most sensitive was found in gentamicin (50%) and the highest resistances were observed in nalidixic acid (90%), erythromycin (90%), and cefotaxime (90%). DNA fi ngerprinting was able to identify the genetic patterns of 18 and 15 in the form of bands 22 and 17 by RAPD-PCR technique with primers 272 and 277, respectively. RAPD-PCR is a technique for genotyping patterns with high resolution in epidemiological studies and bacterial polymorphism. The results of this study showed that antibiotic resistance in isolates of P. aeruginosa is on the rise and there are different patterns in the hospital wards. The children's ward had just common genetic patterns with other wards of the hospital; this was the result of contamination in other parts of the hospital. Thus, according to the data of this study, there is a serious need to control the sources of infections by physicians and staff when they are working in several sectors in order to control and prevent the transmission of the bacteria.

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Section: Resultsmentioning

confidence: 99%

Comparison of genetic and drug resistance patterns in Pseudomonas aeruginosa isolated from the burn wound patients using primers 272 and 277

Mahmmudi¹,

Gorzin²,

Emami³

2016

Biosci. Biotech. Res. Comm

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“…Although molecular approaches for the characterization of virulent genes of UTI organisms have been performed by various research groups [27][28][29][30][31], need to develop a multiplex detection for UTI has not been looked into. To our knowledge, this is the first report that demonstrates an mPCR-based approach for the detection of four major uropathogens in a single reaction tube.…”

Section: Discussionmentioning

confidence: 99%

Rapid and Sensitive Detection of Major Uropathogens in a Single-Pot Multiplex PCR Assay

et al. 2012

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Urinary tract infection (UTI) is among the most common bacterial infections and poses a significant healthcare burden. Escherichia coli is the most common cause of UTI accounting for up to 70 % and a variable contribution from Proteus mirabilis, Pseudomonas aeruginosa and Klebsiella pneumoniae. To establish a complete diagnostic system, we have developed a single-tube multiplex PCR assay (mPCR) for the detection of the above-mentioned four major uropathogens. The sensitivity of the assay was found to be as low as 10(2) cfu/ml of cells. The mPCR evaluated on 280 clinical isolates detected 100 % of E. coli, P. aeruginosa, P. mirabilis and 95 % of K. pneumonia. The assay was performed on 50 urine samples and found to be specific and sensitive for clinical diagnosis. In addition, the mPCR was also validated on spiked urine samples using 40 clinical isolates to demonstrate its application under different strain used in this assay. In total, mPCR reported here is a rapid and simple screening tool that can compete with conventional biochemical-based screening assays that may require 2-3 days for detection.

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“…BLB isolates were first propagated using a modified procedure developed by Akanji et al (2011). Nutrient broth (75 ml; pH 7.5) was prepared inside a 100 ml conical flask.…”

Section: Isolates Propagationmentioning

confidence: 99%

“…This analysis was performed according to Akanji et al (2011). DNA primers used were purchased from Operon Technologies (Alameda, CA, USA) and each was ten nucleotides long.…”

Section: Rapd-pcr Analysismentioning

confidence: 99%

“…Recent research has provided considerable evidence that the deployment of bacterial antagonists to Xoo might be an effective strategy, bringing about disease suppression by biological control (Gnanamanickam, 2009).To understand the epidemiology and ecology of Xoo pathogens and their potential for virulence change, various phenotypic characters as well as molecular markers have been used in studies of Xoo pathogen population structure (Jiang et al, 2006). Identification and classification of bacteria are normally carried out by morphology, nutritional requirements, antibiotic resistance, isozyme comparisons, phage sensitivity (Akanji et al, 2011;Chaudhary et al, 2012) and more recently by DNA based methods, particularly rRNA sequences (Anzai et al, 2002;Chandrashekar et al, 2012), strain-specific fluorescent oligonucleotides (Zhao et al, 2007) and the polymerase chain reaction (PCR) (Akanji et al, 2011). Several repetitive elements found in the Xoo pathogen have been used as probes in restriction fragment length polymorphism (RFLP) analysis (Gonzalez et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

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Two genotypes of Xanthomonas oryzae pv. oryzae virulence identified in West Africa

Amos¹

2013

Int. J. Genet. Mol. Biol.

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Bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae (Xoo), is a very destructive rice disease worldwide. The aim of the present study was to examine if the Xoo virulence pathotypes obtained using phenotypic pathotyping could be confirmed using molecular approach. After screening of 60 Operon primers with genomic DNA of two Xoo isolates (virulent pathotype, Vr and mildly virulent pathotype, MVr), 12 Operon primers that gave reproducible and useful genetic information were selected and used to analyze 50 Xoo isolates from 7 West African countries. Genetic analysis revealed two major Xoo virulence molecular type (Mt) which were Mta and Mtb with Mta having two subgroups (Mta1 and Mta2). Mta1 (Vr1) subgroup genotype has occurrence in six countries and Mta2 (Vr2) in three countries while Mtb genotype characterized mildly virulence (MVr) Xoo isolates present in five countries. The study revealed possible linkage and correlation between phenotypic pathotyping and molecular typing of Xoo virulence. Durable resistance rice cultivars would need to overcome both Mta and Mtb Xoo virulence genotypes in order to survive after their deployment into different rice ecologies in West Africa.

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Genetic Fingerprinting of Pseudomonas aeruginosa Involved in Nosocomial Infection as Revealed by RAPD-PCR Markers

Cited by 14 publications

References 13 publications

Comparison of genetic and drug resistance patterns in Pseudomonas aeruginosa isolated from the burn wound patients using primers 272 and 277

Comparison of genetic and drug resistance patterns in Pseudomonas aeruginosa isolated from the burn wound patients using primers 272 and 277

Rapid and Sensitive Detection of Major Uropathogens in a Single-Pot Multiplex PCR Assay

Two genotypes of Xanthomonas oryzae pv. oryzae virulence identified in West Africa

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