Genetic Heterogeneity in
            <i>pbp</i>
            Genes among Clinically Isolated Group B Streptococci with Reduced Penicillin Susceptibility

Nagano, Noriyuki; Nagano, Yukiko; Kimura, Kouji; Tamai, Kazuya; Yanagisawa, Hideji; Arakawa, Yoshichika

doi:10.1128/aac.00596-08

Cited by 79 publications

(88 citation statements)

References 38 publications

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Section: B-lactammentioning

confidence: 76%

“…However, in view of recent reports of reduced susceptibility to penicillin in some GBS isolates (Chu et al, 2007;Dahesh et al, 2008;Kimura et al, 2008;Nagano et al, 2008), these data suggest that further regular surveillance studies should be carried out on b-lactam susceptibility in GBS isolates.…”

Section: B-lactammentioning

confidence: 76%

“…of strains (and % of total) for which the MIC value (mg ml "1 ) was: Although the isolates were susceptible to the b-lactam antibiotics tested, the MICs of penicillin, ampicillin and cefotaxime for the majority of them were in the range 0.047-0.094 mg ml -1 , similar to the results of a previous study on GBS isolated in the same hospital in 2003 (AlSweih et al, 2005b). However, in view of recent reports of reduced susceptibility to penicillin in some GBS isolates (Chu et al, 2007;Dahesh et al, 2008;Kimura et al, 2008;Nagano et al, 2008), these data suggest that further regular surveillance studies should be carried out on b-lactam susceptibility in GBS isolates.In contrast to the resulsts for b-lactams, the 12.6 % erythromycin-resistance rate in the present study represents a significant increase (P,0.001) in the proportion of erythromycin-resistant isolates from the 0.7 % prevalence reported in GBS isolates obtained from patients in the same hospital in 2003 (Al-Sweih et al, 2005b). This increase may be related to the increased use of macrolides in the hospital.…”

mentioning

confidence: 76%

Section: Introductionmentioning

confidence: 99%

“…As GBS isolates remain uniformly susceptible to penicillin, its prophylactic use has been instrumental in significantly reducing the incidence of early-onset diseases in neonates (Pylipow et al, 1994). However, GBS isolates with reduced susceptibility to penicillin have recently been reported (Chu et al, 2007;Dahesh et al, 2008;Kimura et al, 2008;Nagano et al, 2008).Erythromycin and clindamycin are used as alternative treatments for patients who are allergic to penicillin. However, erythromycin and clindamycin resistance have emerged in several countries (Aracil et al, 2002;Betriu et al, 2003;de Azavedo et al, 2001;De Mouy et al, 2001).…”

mentioning

confidence: 99%

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Serotypes and antibiotic resistance in Group B streptococcus isolated from patients at the Maternity Hospital, Kuwait

Boswihi

Udo

Al‐Sweih

2012

Journal of Medical Microbiology

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A total of 143 group B streptococcus (GBS) isolates collected from mothers at the Maternity Hospital in Kuwait were investigated for their serotypes and antibiotic resistance, and screened by PCR for the carriage of genes for resistance to tetracycline (tetk, tetM, tetL, tetO), erythromycin (ermA, ermB, ermC, ermTR, ermM, mefA, mefE, msrA) and aminoglycosides (aph3, ant4, ant6). All isolates were serotyped using a latex agglutination test. Most of the isolates belonged to serotypes V (38.5 %), III (20.9 %), Ia (7.7 %) and II (11.2 %). Sixteen isolates (11.2 %) were nontypable. All isolates were susceptible to penicillin, ampicillin and cefotaxime (MICs 0.016-0.094 mg ml "1 ) but were resistant to trimethoprim (92.3 %), tetracycline (89.5 %), minocycline (89.5 %), high-level kanamycin (76.9 %), chloramphenicol (30.0 %), erythromycin (12.6 %), clindamycin (7.0 %), high-level streptomycin (3.5 %) and ciprofloxacin (0.7 %). The tetracycline-resistant isolates contained tetM (94.5 %), tetO (3.9 %), tetL (1.6 %) and tetK (0.8 %). The erythromycin-resistant isolates contained ermB (61.1 %), ermTR (38.9 %), ermA (5.5 %), mefA (5.5 %) and mefE (11 %). All high-level kanamycin-resistant isolates contained aph3. One of the high-level streptomycin-resistant isolates contained ant6. Partial DNA sequencing of aph3 revealed sequences with 99 % similarity to aph3 found in Enterococcus faecium, Enterococcus faecalis, Staphylococcus aureus and Staphylococcus epidermidis, suggesting that the GBS isolates could have acquired aph3 from other Gram-positive cocci. The high proportion of isolates with resistance to tetracycline, high-level kanamycin and trimethoprim, and the increase in the prevalence of erythromycin resistance, represents an emerging public health concern that needs further surveillance. INTRODUCTIONGroup B streptococcus (GBS), also known as Streptococcus agalactiae, is a leading cause of serious infections in neonates and immunocompromised adults (Truog et al., 1976;Baker, 1979;High et al., 2005).GBS are encapsulated and have been classified into ten serotypes (Ia, Ib, II, III, IV, V, VI, VII, VIII and IX) on the basis of the capsular polysaccharide components of their cell wall (Victor & Rubens, 2000;Slotved et al., 2007). The capsular polysaccharide, as a virulence factor, assists GBS survival in vivo by avoiding recognition by the host defence system (Rubens et al., 1987). There are major differences between countries in the distribution of GBS serotypes. In the USA, Germany and Sweden, the most common serotypes isolated from infants and colonized mothers have been types Ia, III, and V (Persson et al., 2004;Brimil et al., 2006;Harrison et al., 1998). Serotype V strains emerged in the 1990s as a frequent cause of neonatal sepsis and adult diseases in the USA. On the other hand, serotypes VI and VIII represented 35.6 % and 24.7 %, respectively, of GBS isolated from pregnant women in Japan (Lachenauer et al., 1999).Penicillin G is the drug of choice for the intrapartum prophylaxis of GBS infections (Figueira-Coelho e...

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Section: B-lactammentioning

confidence: 76%

Section: B-lactammentioning

confidence: 76%

mentioning

confidence: 76%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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Serotypes and antibiotic resistance in Group B streptococcus isolated from patients at the Maternity Hospital, Kuwait

Boswihi

Udo

Al‐Sweih

2012

Journal of Medical Microbiology

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β‐Lactam Antibiotics

Testero

Fisher

Mobashery

2010

Burger's Medicinal Chemistry and Drug Discovery

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The β‐lactam classes of antibacterials are preeminent in the treatment of bacterial infection due to their unparalleled clinical efficacy and clinical safety. Following the discovery of the penicillins, successive β‐lactam drug discovery has added the cephalosporin, penem cephamycin, clavulanate, monobactam, nocardicin, and carbapenem subclasses. The driving force behind much of this era of discovery is the staggering ability of pathogenic bacteria to adapt previous generations of the β‐lactam by the acquisition and expression of resistance mechanisms. Although many factors contribute to β‐lactam resistance, alterations to the molecular targets of the β‐lactams (the penicillin binding proteins) and the use of enzymes (the β‐lactamases) capable of the hydrolytic deactivation of the β‐lactams are paramount. This review traces the historical development of β‐lactam drug discovery, with emphasis on the most recent progress in the medicinal chemistry, biochemistry, and microbiology of the β‐lactams leading to the discovery of new generation β‐lactam antibacterials effective against the Gram‐negative and ‐positive bacterial pathogens of current medical concern.

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The overlooked bacterial pandemic

Moriel,

Piccioli,

Raso

et al. 2023

Semin Immunopathol

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The COVID-19 pandemic had a significant economic and health impact worldwide. It also reinforced the misperception that only viruses can pose a threat to human existence, overlooking that bacteria (e.g., plague and cholera) have severely haunted and shaped the course of human civilization. While the world is preparing for the next viral pandemic, it is again overlooking a silent one: antimicrobial resistance (AMR). This review proposes to show the impact of bacterial infections on civilization to remind the pandemic potential. The work will also discuss a few examples of how bacteria can mutate risking global spread and devastating outcomes, the effect on the global burden, and the prophylactic and therapeutic measures. Indeed, AMR is dramatically increasing and if the trend is not reversed, it has the potential to quickly turn into the most important health problem worldwide.

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Genetic Heterogeneity in pbp Genes among Clinically Isolated Group B Streptococci with Reduced Penicillin Susceptibility

Cited by 79 publications

References 38 publications

Serotypes and antibiotic resistance in Group B streptococcus isolated from patients at the Maternity Hospital, Kuwait

Serotypes and antibiotic resistance in Group B streptococcus isolated from patients at the Maternity Hospital, Kuwait

β‐Lactam Antibiotics

The overlooked bacterial pandemic

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