Genetic Heterogeneity of a Diploid Grass Aegilops tauschii Revealed by Chromosome Banding Methods and Electrophoretic Analysis of the Seed Storage Proteins (Gliadins)

Бадаева, Е. Д.; Фисенко, А. В.; Суржиков, С. А.; Yankovskaya, A. A.; Chikida, N. N.; Zoshchuk, Svyatoslav A.; Belousova, Maria Kh.; Dragovich, A. Yu.

doi:10.1134/s1022795419110024

Cited by 9 publications

(10 citation statements)

References 74 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…tauschii [120][121][122]. The results of molecular [103,123,124] and cytogenetic [125,126] studies suggested that the wheat D genome was contributed by Ae. tauschii subsp.…”

Section: Evolution and Domestication Historymentioning

confidence: 99%

Introducing Beneficial Alleles from Plant Genetic Resources into the Wheat Germplasm

Sharma¹,

Schulthess

Bassi³

et al. 2021

Biology

View full text Add to dashboard Cite

Wheat (Triticum sp.) is one of the world’s most important crops, and constantly increasing its productivity is crucial to the livelihoods of millions of people. However, more than a century of intensive breeding and selection processes have eroded genetic diversity in the elite genepool, making new genetic gains difficult. Therefore, the need to introduce novel genetic diversity into modern wheat has become increasingly important. This review provides an overview of the plant genetic resources (PGR) available for wheat. We describe the most important taxonomic and phylogenetic relationships of these PGR to guide their use in wheat breeding. In addition, we present the status of the use of some of these resources in wheat breeding programs. We propose several introgression schemes that allow the transfer of qualitative and quantitative alleles from PGR into elite germplasm. With this in mind, we propose the use of a stage-gate approach to align the pre-breeding with main breeding programs to meet the needs of breeders, farmers, and end-users. Overall, this review provides a clear starting point to guide the introgression of useful alleles over the next decade.

show abstract

“…tauschii [120][121][122]. The results of molecular [103,123,124] and cytogenetic [125,126] studies suggested that the wheat D genome was contributed by Ae. tauschii subsp.…”

Section: Evolution and Domestication Historymentioning

confidence: 99%

Introducing Beneficial Alleles from Plant Genetic Resources into the Wheat Germplasm

Sharma¹,

Schulthess

Bassi³

et al. 2021

Biology

View full text Add to dashboard Cite

show abstract

“…The genome composition of each species is indicated in between brackets and the reticulation event between species of the A‐ and the B‐genome lineages at the origin of the D‐genome lineage is shown as dotted lines following Huynh et al (), with mean node ages and 95% credibility intervals in Myr ago (Ma). Chromosomes were labelled with the A/D‐genome‐specific repeat pAs1 (green) and the B/S‐genome‐specific repeat pSc119.2 (red), and fluorescence in situ hybridization (FISH) karyotypes following Badaeva et al (, , ) and Ruban & Badaeva () show progenitor species from the A‐genome ( T. monococcum here shown) and B‐genome ( Ae. speltoides here shown) lineages together with selected karyotypes from the three clades nested within the hybrid D‐genome lineage: (1) Aegilops tauschii shows the A‐like karyotype of the tauschii clade (also see Fig.…”

Section: Reticulate Evolution Of Diploid Wild Wheatsmentioning

confidence: 99%

Chromosome restructuring among hybridizing wild wheats

Parisod

Бадаева

2020

New Phytologist

View full text Add to dashboard Cite

Summary The wheat group offers an outstanding system to address the interplay between hybridization, chromosomal evolution and biological diversification. Most diploid wild wheats originated following hybridization between the A‐genome lineage and the B‐genome lineage some 4 Myr ago, resulting in an admixed D‐genome lineage that presented dramatic radiation accompanied by considerable changes in genome size and chromosomal rearrangements. Comparative profiling of low‐copy genes, repeated sequences and transposable elements among those divergent species characterized by different karyotypes highlights high genome dynamics and sheds new light on the processes underlying chromosomal evolution in wild wheats. One of the hybrid clades presents upsizing of metacentric chromosomes going along with the proliferation of specific repeats (i.e. ‘genomic obesity’), whereas other species show stable genome size associated with increasing chromosomal asymmetry. Genetic and ecological variation in those specialized species suggest that genome restructuring was coupled with adaptive processes to support the evolution of a majority of acrocentric chromosomes. This synthesis of current knowledge on genome restructuring across the diversity of wild wheats paves the way towards surveys based on latest sequencing technologies to characterize valuable resources and address the significance of chromosomal evolution in species with complex genomes.

show abstract

“…The A subgenome chromosomes of wheat were classified using additional probe combination GAA n and pTa-535 according to Komuro et al (2013) ; chromosomes of Ae . Tauschii were classified as suggested by Badaeva et al (2002 , 2019a) and Zhao et al (2018) , Ae . crassa as in Abdolmalaki et al (2019) , and Badaeva et al (2021) .…”

Section: Methodsmentioning

confidence: 99%

“…strangulata ), PI 201890 ( Th . bessarabicum ), and Chinese Spring have normal karyotypes typical to the respective species ( Gill et al, 1991 ; Badaeva et al, 2019a , 2019b , 2021 ). At the same time, we have found earlier that hexaploid Ae .…”

Section: Methodsmentioning

confidence: 99%

“…The D subgenome is also present in two polyploid Aegilops species and in four exaploidy bread wheat Triticum aestivum (2n = 6x = BBAADD). According to molecular analysis of nuclear genome ( Dvořák et al, 1998 ; Luo et al, 2017 ; Singh et al, 2019 ) and hybridization pattern of pAs1 probe ( Badaeva et al, 1996 , 2019a ; Zhao et al, 2018 ; Ebrahimzadegan et al, 2021 ), the D subgenome of polyploid wheat was inherited from Ae . tauschii subsp.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Aegilops crassa Boiss. repeatome characterized using low-coverage NGS as a source of new FISH markers: Application in phylogenetic studies of the Triticeae

et al. 2022

View full text Add to dashboard Cite

Aegilops tauschii Boiss. is polyploid grass species that grows in the eastern part of the Fertile Crescent, Afghanistan, and Middle Asia. It consists of tetraploid (4x) and hexaploid (6x) cytotypes (2n = 4x = 28, D1D (Abdolmalaki et al., 2019) XcrXcr and 2n = 6x = 42, D1D (Abdolmalaki et al., 2019) XcrXcrD2D (Adams and Wendel, 2005), respectively) that are similar morphologically. Although many Aegilops species were used in wheat breeding, the genetic potential of Ae. crassa has not yet been exploited due to its uncertain origin and significant genome modifications. Tetraploid Ae. crassa is thought to be the oldest polyploid Aegilops species, the subgenomes of which still retain some features of its ancient diploid progenitors. The D1 and D2 subgenomes of Ae. crassa were contributed by Aegilopstauschii (2n = 2x = 14, DD), while the Xcr subgenome donor is still unknown. Owing to its ancient origin, Ae. crassa can serve as model for studying genome evolution. Despite this, Ae. crassa is poorly studied genetically and no genome sequences were available for this species. We performed low-coverage genome sequencing of 4x and 6x cytotypes of Ae. crassa, and four Ae. tauschii accessions belonging to different subspecies; diploid wheatgrass Thinopyrum bessarabicum (Jb genome), which is phylogenetically close to D (sub)genome species, was taken as an outgroup. Subsequent data analysis using the pipeline RepeatExplorer2 allowed us to characterize the repeatomes of these species and identify several satellite sequences. Some of these sequences are novel, while others are found to be homologous to already known satellite sequences of Triticeae species. The copy number of satellite repeats in genomes of different species and their subgenome (D1 or Xcr) affinity in Ae. crassa were assessed by means of comparative bioinformatic analysis combined with quantitative PCR (qPCR). Fluorescence in situ hybridization (FISH) was performed to map newly identified satellite repeats on chromosomes of common wheat, Triticum aestivum, 4x and 6x Ae. crassa, Ae. tauschii, and Th. bessarabicum. The new FISH markers can be used in phylogenetic analyses of the Triticeae for chromosome identification and the assessment of their subgenome affinities and for evaluation of genome/chromosome constitution of wide hybrids or polyploid species.

show abstract

Genetic Heterogeneity of a Diploid Grass Aegilops tauschii Revealed by Chromosome Banding Methods and Electrophoretic Analysis of the Seed Storage Proteins (Gliadins)

Cited by 9 publications

References 74 publications

Introducing Beneficial Alleles from Plant Genetic Resources into the Wheat Germplasm

Introducing Beneficial Alleles from Plant Genetic Resources into the Wheat Germplasm

Chromosome restructuring among hybridizing wild wheats

Aegilops crassa Boiss. repeatome characterized using low-coverage NGS as a source of new FISH markers: Application in phylogenetic studies of the Triticeae

Contact Info

Product

Resources

About