2007
DOI: 10.1007/s00436-007-0574-z
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Genetic heterogeneity of Fasciola hepatica isolates in the northwest of Spain

Abstract: The liver fluke Fasciola hepatica is a worldwide distributed trematode parasite of great veterinary importance. In Spain, the prevalence of the infection is up to 56% in sheep. Its control is mainly based on the use of anthelmitics, although some resistant populations have been described. Genetic studies of the genus Fasciola have been focused on the 28S ribosomal deoxyribonucleic acid (rDNA) gene, with no intraspecific variations and a few interspecific nucleotide differences. We have designed a specific poly… Show more

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Cited by 27 publications
(26 citation statements)
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“…Previous studies have characterized Fasciola from different countries and different regions of Iran using genetic approaches (15,(20)(21)(22)(23)(24)(25). However, before the present study, there have been no molecular investigations regarding the Fasciola species from Sistan and Balouchestan province.…”
Section: Discussionmentioning
confidence: 82%
“…Previous studies have characterized Fasciola from different countries and different regions of Iran using genetic approaches (15,(20)(21)(22)(23)(24)(25). However, before the present study, there have been no molecular investigations regarding the Fasciola species from Sistan and Balouchestan province.…”
Section: Discussionmentioning
confidence: 82%
“…Most commonly utilized variable regions are ITS 1, ITS 2 and D-domains (divergent regions in 28S and 18S genes). As it concerns F. hepatica many research teams have applied above mentioned rDNA genes in multiple investigations (Lee et al, 2007;Olson et al, 2003;Lotfy et al, 2008;Vara-Del Río et al, 2007;Marcilla et al, 2002, etc.). D1-region from 28S rDNA has been used for genetic structuring of the populations of species from phylum Platyhelmintes (Lee et al, 2007).…”
Section: Ribosomal Dna Regions Used For Snp Assaymentioning
confidence: 99%
“…Using the nucleotide sequences and phylogenetic relationships of mentioned ribosomal regions the authors described evolutionary processes that occurred with fasciolid species and defined their origin. A fragment from 28S rDNA has been used to demonstrate the genetic heterogeneity of F. hepatica isolates in Spain (Vara-Del Río et al, 2007). SNP assay of that fragment sequence revealed intraspecific genetic variability.…”
Section: Ribosomal Dna Regions Used For Snp Assaymentioning
confidence: 99%
See 1 more Smart Citation
“…Over the past few years, many efforts focused on studying the population genetic structure and heterogeneity of important trematode species including Schistosoma mansoni [25][26][27][28][29][30], S. japonicum [31][32][33], Paragonimus westermani [34,35], Fasciola hepatica [36][37][38][39][40], F. magnum [41] Echinostoma sp. [42,43].…”
Section: Introductionmentioning
confidence: 99%