Genetic heterogeneity of porcine enteric caliciviruses identified from diarrhoeic piglets

Martella, Vito; Bányai, Krisztiàn; Lorusso, Eleonora; Bellacicco, Anna Lucia; Decaro, Nicola; Mari, Viviana; Saif, Linda J.; Costantini, Verónica; Grazia, Simona De; Pezzotti, Giovanni; Llombart‐Bosch, Antonio; Buonavoglia, Canio

doi:10.1007/s11262-008-0198-0

Cited by 46 publications

(43 citation statements)

References 39 publications

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“…The country origin of the strains is indicated by color coding (Denmark, red; Finland, light blue; Hungary, blue; Italy, purple; Slovenia, green; Spain, yellow). To maintain the continuity of the current nomenclature for sapovirus genogrouping, we used the previously proposed taxonomic names as a basis (14,25). Sapoviruses in genogroups GI, GII, GIV, and GV are detected in humans.…”

Section: Resultsmentioning

confidence: 99%

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Incidence, Diversity, and Molecular Epidemiology of Sapoviruses in Swine across Europe

et al. 2010

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Porcine sapovirus is an enteric calicivirus in domestic pigs that belongs to the family Caliciviridae. Some porcine sapoviruses are genetically related to human caliciviruses, which has raised public health concerns over animal reservoirs and the potential cross-species transmission of sapoviruses. We report on the incidence, genetic diversity, and molecular epidemiology of sapoviruses detected in domestic pigs in a comprehensive study conducted in six European countries (Denmark, Finland, Hungary, Italy, Slovenia, and Spain) between 2004 and 2007. A total of 1,050 swine fecal samples from 88 pig farms were collected and tested by reverse transcription-PCR for sapoviruses, and positive findings were confirmed by sequencing. Sapoviruses were detected in 80 (7.6%) samples collected on 39 (44.3%) farms and in every country. The highest prevalence was seen among piglets aged 2 to 8 weeks, and there was no significant difference in the proportion of sapoviruspositive findings for healthy animals and animals with diarrhea in Spain and Denmark (the only countries where both healthy animals and animals with diarrhea were tested). On the basis of the sequence of the RNA polymerase region, highly heterogeneous populations of viruses representing six different genogroups (genogroups III, VI, VII, and VIII, including potential new genogroups IX and X) were identified, with a predominance of genogroup GIII (50.6%). Genogroup VIII, found in five of the six countries, had the highest degree of homology (up to 66% at the amino acid level) to human sapovirus strains. Sapoviruses are commonly circulating and endemic agents in swine herds throughout Europe. Highly heterogeneous and potential new genogroups of sapoviruses were found in pigs; however, no "human-like" sapoviruses were detected.

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Section: Resultsmentioning

confidence: 99%

“…Human sapoviruses belong to genogroups GI, GII, GIV, and GV, whereas porcine sapovirus belongs to genogroup GIII. Recently, new porcine sapovirus genogroups (genogroups GVI, GVII, and GVIII) were proposed (14,25,27). Recombinant sapoviruses have also been described in both human and swine hosts (8,24).…”

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Incidence, Diversity, and Molecular Epidemiology of Sapoviruses in Swine across Europe

et al. 2010

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“…Porcine SaVs have been detected in several European countries (Reuter et al, 2007(Reuter et al, , 2010Martella et al, 2008;Mauroy et al, 2008;Collins et al, 2009), Asia (Kim et al, 2006;Yin et al, 2006;Yang et al, 2009) and the Americas (Martinez et al, 2006;Wang et al, 2006;Barry et al, 2008;L'Homme et al, 2009), indicating their worldwide distribution. Nevertheless, there is still a lack of information regarding SaV epidemiology because porcine SaVs are not included in the routine diagnosis of porcine pathogens (Collins et al, 2009).…”

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confidence: 99%

“…For the detection of sapoviral RNA in faecal samples, RT-PCR based on the detection of RNA dependent RNA polymerase (RdRp) or capsid genes has been used in epidemiological studies (Jiang et al, 1999;Guo et al, 2001;Kim et al, 2006). The results of RT-PCR analysis of faecal samples from diarrhoeic piglets suggest that sapoviruses are associated with diarrhoea in piglets either alone or in combination with other enteric pathogens (Jeong et al, 2007;Reuter et al, 2007;Martella et al, 2008;Zhang et al, 2008). Using RT-PCR methods, porcine sapoviruses have been detected in both symptomatic and asymptomatic animals (Reuter et al, 2010).…”

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confidence: 99%

“…Porcine SaVs are divided into four genogroups (GIII, GVI, GVII, GVIII) based on complete capsid gene sequences (Martella et al, 2008;Wang et al, 2005;Yin et al, 2006); nevertheless, the majority of porcine sapoviruses including the prototype strain Cowden, belong to GIII (Guo et al, 1999). Recently, two potential new genogroups, GIX and GX, were described by Reuter et al (2010).…”

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Molecular characterization of a porcine sapovirus strain isolated from a piglet with diarrhoea: a case report

Dufková¹,

Kulich²,

Prodělalová³

2011

Vet. Med. - Czech

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Porcine sapoviruses, members of the family Caliciviridae, have been considered as an aetiological agent of gastroenteritis in pigs. In this study, we analysed 251 faecal samples obtained from 3 to 90 day-old diarrhoeic pigs in the Czech Republic between January 2005 and June 2010 and tested them by negative staining electron microscopy for the presence of sapoviruses. Only one sample showed the presence of viral particles with characteristic sapovirus morphology. The presence of sapovirus (SaV) was confirmed by an RT-PCR assay with primers specific for the sapoviral RNA polymerase and capsid genes. Phylogenetic analysis based on a partial sequence of the RNA polymerase gene placed the new Czech isolate into the GVII genogroup of porcine sapoviruses; however, analysis of a portion of the capsid gene sequence classified the isolate as GIII of the genus Sapovirus. These contradictory findings indicate that recombinant porcine sapovirus was identified. According to our knowledge this is the first description of porcine sapovirus in domestic pigs in the Czech Republic.

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Noroviruses and sapoviruses in pigs in Belgium

et al. 2008

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Porcine noroviruses and sapoviruses belong to the family Caliciviridae and are rarely reported in European countries. In this study, swine stools from a region representative of northern Europe were screened for these viruses by RT-PCR. Both porcine noroviruses and sapoviruses were detected, showing their circulation in this region. The porcine norovirus strains were genetically related to genotype 19 strains in the genogroup II of the genus Norovirus. The porcine sapovirus strains were genetically related to the porcine enteric calicivirus Cowden reference strain and to newly described porcine strains in the genus Sapovirus.

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Genetic heterogeneity of porcine enteric caliciviruses identified from diarrhoeic piglets

Cited by 46 publications

References 39 publications

Incidence, Diversity, and Molecular Epidemiology of Sapoviruses in Swine across Europe

Incidence, Diversity, and Molecular Epidemiology of Sapoviruses in Swine across Europe

Molecular characterization of a porcine sapovirus strain isolated from a piglet with diarrhoea: a case report

Noroviruses and sapoviruses in pigs in Belgium

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