Genetic identification and hybridization in the seagrass genus<i>Halophila</i>(Hydrocharitaceae) in Sri Lankan waters

Liu, Shang-Yin Vanson; Kumara, Terney Pradeep; Hsu, Chi-Hsuan

doi:10.7717/peerj.10027

Cited by 16 publications

(12 citation statements)

References 37 publications

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“…ramamurthiana in India there were no nucleotide differences found (Nguyen et al 2013). A recent study on the genus Halophila in Sri Lanka using ITS sequences showed a clade of Halophila ovalis complex which consisted of H. ovata, H. minor, H. ovalis, H. hawaiiana, and H. johnsonii (Liu et al 2020).…”

Section: Halodule Pinifoliamentioning

confidence: 87%

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Biodiversity and phylogenetic analyses using DNA barcoding rbcL gene of seagrass from Sekotong, West Lombok, Indonesia

Stevanus

Pharmawati

2020

Biodiversitas

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Abstract. Stevanus, Pharmawati M. 2021. Biodiversity and phylogenetic analyses using DNA barcoding rbcL gene of seagrass from Sekotong, West Lombok, Indonesia. Biodiversitas 22: 50-57. West Lombok, Indonesia is one of the locations that is thought to have a quite high diversity of seagrass. Information on the diversity of seagrass species is important due to the important value of seagrass in the marine ecosystem. This research aimed to analyze biodiversity and phylogenetic of seagrass from Sekotong, West Lombok, Indonesia using DNA barcoding of rbcL gene. As many as 35 samples from seven morphologically identified species (Enhalus acoroides, Thalassia hemprichii, Cymodocea rotundata, Syringodium isoetifolium, Halodule pinifolia, Halophila ovalis, and H. minor) were taken from four Gilis (small island) in Sekotong. The DNA was amplified for the rbcL gene and sequence analyses using BLAST were conducted to determine the species. Phylogenetic analyses were carried out using three evolutionary algorithms using Neighbor-Joining, Maximum Likelihood and Bayesian analysis with 1000 bootstrap. The rbcL gene was successfully amplified from all samples with a maximum length of 552 bp. The phylogenetic analysis showed that clades were split by family and genera where six clades were formed (Enhalus acoroides, T. hemprichii, Halophila complex, H. pinifolia, S. isoetifolium, and C. rotundata) with more than 95% of bootstrap values for Neighbor-Joining and Bayesian. The p-distance values between species were 0.008-0.097 and the polymorphic site was not found within species. The rbcL sequences only confirmed five seagrass species out of seven morphologically identified species and the sequences generated from this study cannot discriminate Halophila ovalis and H. minor.

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Section: Halodule Pinifoliamentioning

confidence: 87%

“…The rbcL region has been used to differentiate four seagrass species from Thailand (Osathanunkul et al 2015), identify Halophila spp. from Sri Lanka (Liu et al 2020), and identify other flowering plants such as Sonchus arvensis (Wahyuni et al 2019) and genus Piper (Naim and Mahboob 2020).…”

Section: Introductionmentioning

confidence: 99%

Biodiversity and phylogenetic analyses using DNA barcoding rbcL gene of seagrass from Sekotong, West Lombok, Indonesia

Stevanus

Pharmawati

2020

Biodiversitas

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“…Indeed, H. x tanabensis as a hybrid between H. nipponica and H. major was described [ 56 ]. More recently, a hybrid between H. ovalis and H. major has been demonstrated molecularly from Sri Lanka [ 75 ]. However, using new sequencing methods recommended by Yu et al [ 76 ] may resolve these conspecific species’ complexity and assist in future seagrass taxonomical research.…”

Section: Discussionmentioning

confidence: 99%

“…It is anticipated that some of the described Halophila species in this review may become synonyms after more studies are conducted in the future. On the other hand, there are potentially more new species in the Halophila yet to be described [ 75 , 83 ]. It has been suggested that the new species should be accepted if it is supported by genetic data [ 84 ].…”

Section: Discussionmentioning

confidence: 99%

Taxonomy of the Genus Halophila Thouars (Hydocharitaceae): A Review

Kuo

2020

Plants

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The seagrass genus Halophila Thouars has more than twenty described species and is predominately distributed over a wide geographic range along the tropical and the warm temperate coastlines in the Indo-West Pacific Oceans. A brief history of the Halophila taxonomic development is presented. Based on reproductive and vegetative morphology, the genus is divided into eight sections including three new sections: section Australes, section Stipulaceae and section Decipientes. A rewritten taxonomic description of the type species for the genus Halophila,H. madagascariensis Steudel ex Doty et B.C. Stone, is provided. The lectotype of H. engelmannii Asch. as well as neotypes of H. hawaiiana Doty et B.C. Stone and H. spinulosa (Br.) Asch. are designated. Furthermore, H. ovalis ssp. bullosa, ssp. ramamurthiana and ssp. linearis together with H. balforurii have been recognised as distinct species. Nomenclature, typification, morphological description and botanical illustrations are presented for each taxon. Recent molecular phylogenetic surveys on certain Halophila taxa are also discussed. Field surveys for the deep water Halophila in West Pacific regions are suggested. Morphological studies combined with molecular investigations for the Halophila on the east coast of Africa and the West Indian Ocean are urgently needed and highly recommended.

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“…The high morphological plasticity of species and the presence of locally adapted morphotypes could lead to erroneous species identification. This could favor in turn, the hybridization with native species, the breakdown of locally adapted ecotypes, and the establishment of hybrids (i.e., genetic swamping), potentially compromising the entire ecosystem functioning [98]. In this case, species identification should also be performed at a genetic level to overcome taxonomic ambiguity.…”

Section: Speciesmentioning

confidence: 99%

The Genetic Component of Seagrass Restoration: What We Know and the Way Forwards

Pazzaglia

Nguyen

Santillán-Sarmiento

et al. 2021

Water

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Seagrasses are marine flowering plants providing key ecological services and functions in coasts and estuaries across the globe. Increased environmental changes fueled by human activities are affecting their existence, compromising natural habitats and ecosystems’ biodiversity and functioning. In this context, restoration of disturbed seagrass environments has become a worldwide priority to reverse ecosystem degradation and to recover ecosystem functionality and associated services. Despite the proven importance of genetic research to perform successful restoration projects, this aspect has often been overlooked in seagrass restoration. Here, we aimed to provide a comprehensive perspective of genetic aspects related to seagrass restoration. To this end, we first reviewed the importance of studying the genetic diversity and population structure of target seagrass populations; then, we discussed the pros and cons of different approaches used to restore and/or reinforce degraded populations. In general, the collection of genetic information and the development of connectivity maps are critical steps for any seagrass restoration activity. Traditionally, the selection of donor population preferred the use of local gene pools, thought to be the best adapted to current conditions. However, in the face of rapid ocean changes, alternative approaches such as the use of climate-adjusted or admixture genotypes might provide more sustainable options to secure the survival of restored meadows. Also, we discussed different transplantation strategies applied in seagrasses and emphasized the importance of long-term seagrass monitoring in restoration. The newly developed information on epigenetics as well as the application of assisted evolution strategies were also explored. Finally, a view of legal and ethical issues related to national and international restoration management is included, highlighting improvements and potential new directions to integrate with the genetic assessment. We concluded that a good restoration effort should incorporate: (1) a good understanding of the genetic structure of both donors and populations being restored; (2) the analysis of local environmental conditions and disturbances that affect the site to be restored; (3) the analysis of local adaptation constraints influencing the performances of donor populations and native plants; (4) the integration of distribution/connectivity maps with genetic information and environmental factors relative to the target seagrass populations; (5) the planning of long-term monitoring programs to assess the performance of the restored populations. The inclusion of epigenetic knowledge and the development of assisted evolution programs are strongly hoped for the future.

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Genetic identification and hybridization in the seagrass genusHalophila(Hydrocharitaceae) in Sri Lankan waters

Cited by 16 publications

References 37 publications

Biodiversity and phylogenetic analyses using DNA barcoding rbcL gene of seagrass from Sekotong, West Lombok, Indonesia

Biodiversity and phylogenetic analyses using DNA barcoding rbcL gene of seagrass from Sekotong, West Lombok, Indonesia

Taxonomy of the Genus Halophila Thouars (Hydocharitaceae): A Review

The Genetic Component of Seagrass Restoration: What We Know and the Way Forwards

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