Genetic identification of astroviruses in wild boars

Vilček, Štefan; Salamunova, Slavomira; Jacková, Anna

doi:10.4142/jvs.2019.20.1.91

Cited by 5 publications

(6 citation statements)

References 14 publications

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“…Previous studies on AstVs in farmed and companion animals have demonstrated that turkeys, ducks, chickens, guinea fowl, pigeons, geese, mink, sheep, cattle, pigs, and cats are hosts of astroviruses [ 3 , 16 , 17 ]. In the population of wild animals, AstVs were mostly detected in bat samples, with several studies performed in wild boars in Slovakia and Hungary [ 29 , 30 ]. Metagenomics studies performed on rodents in China and red foxes in the Netherlands revealed novel rodent and fox astroviruses, respectively [ 31 , 32 ].…”

Section: Discussionmentioning

confidence: 99%

“…Metagenomics studies performed on rodents in China and red foxes in the Netherlands revealed novel rodent and fox astroviruses, respectively [ 31 , 32 ]. In our study, we screened a broad range of animal species using the method Chu et al [ 19 ] successfully applied for the detection of astroviruses in bats in Poland [ 18 ], in wild boars in Slovakia [ 29 ], and in different wild animals in Italy [ 33 ]. Although astroviruses were most frequently detected in rat samples, with a prevalence of 33.3%, the number of samples tested was only nine due to the low number of rat samples submitted during rabies surveillance.…”

Section: Discussionmentioning

confidence: 99%

“…In the past, there were few suggestions of interspecies transmission of Avastroviruses via feed from infected birds to mink in China [ 13 ]. Avastrovirus has also been detected in cat feces in Florida, while chicken astroviruses and bat astrovirus were identified in wild boars in Slovakia [ 29 ], all without a true origin. In addition, non-human primates were shown to harbor a wide spectrum of mammalian and avian astroviruses [ 37 ].…”

Section: Discussionmentioning

confidence: 99%

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Prevalence of Astroviruses in Different Animal Species in Poland

Kuczera,

Orłowska,

Smreczak

et al. 2024

Viruses

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Astroviruses (AstVs) are small RNA viruses characterized by a high mutation rate, the ability to recombine, and interspecies transmission, which allows them to infect a multitude of hosts including humans, companion animals, and farmed animals as well as wildlife. AstVs are stable in the environment, and their transmission is usually through the fecal–oral route or via contaminated water and food. Although direct zoonotic transmission was not confirmed, interspecies transmission events have occurred or have been indicated to occur in the past between wild and domestic animals and humans. They cause large economic losses, mainly in the poultry sector, due to gastroenteritis and mortality. In young children, they are the second most common cause of diarrhea. This study involved 166 intestine samples and pools of spleen, lymph node, and kidney samples collected from 352 wild animals, 52 pigs, and 31 companion animals. Astroviruses were detected in the intestine samples and were separately detected in pools of tissue samples prepared for individual animals using a heminested RT-PCR protocol. Amplicons were subjected to Sanger sequencing, and a phylogenetic analysis of 320 nt RNA-dependent RNA polymerase (RdRp) fragments referring to known nt sequences of astroviruses was performed. Astroviral RNA was detected in the intestine samples and/or tissue pools of red foxes (nine positive intestines and six positive tissue pools), rats (two positive intestines and three positive tissue pools), a cat (one AstV detected in an intestine sample), pigs (eight positive tissue pools), and wild boars (two positive pools of spleens, kidneys, and lymph nodes). No astroviral RNA was detected in wild mustelids, dogs, or other small wild animals including rodents. A phylogenetic analysis revealed that the astroviruses detected during this study were mostly host-specific, such as porcine, canine, and rat astroviruses that were highly homologous to the sequences of reference strains. In one of two wild boars, an AstV distinct to porcine species was found with the highest nt identity to Avastroviruses, i.e., turkey astroviruses, which suggests potential cross-species transmission of the virus, as previously described. Here, we present the first detection of astroviruses in the population of wild animals, companion animals, and pigs in Poland, confirming that astroviruses are frequent pathogens circulating in animals in the field. Our study also suggests potential cross-species transmission of Avaastrovirus to wild boars; however, further molecular characterization is needed.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Prevalence of Astroviruses in Different Animal Species in Poland

Kuczera,

Orłowska,

Smreczak

et al. 2024

Viruses

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“…Astrovirus wild boar WBAstV-1 8 11 Associated with gastroenteritis. Can be found in boars worldwide (Vilcek et al, 2019).…”

Section: Prrsv Genome Coveragementioning

confidence: 99%

Application of shotgun metagenomics sequencing and targeted sequence capture to detect circulating porcine viruses in the Dutch–German border region

Schuele

Lizarazo-Forero

Strutzberg-Minder³

et al. 2021

Transbounding Emerging Dis

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Porcine viruses have been emerging in recent decades, threatening animal and human health, as well as economic stability for pig farmers worldwide. Next‐generation sequencing (NGS) can detect and characterize known and unknown viruses but has limited sensitivity when an unbiased approach, such as shotgun metagenomics sequencing, is used. To increase the sensitivity of NGS for the detection of viruses, we applied and evaluated a broad viral targeted sequence capture (TSC) panel and compared it to an unbiased shotgun metagenomic approach. A cohort of 36 pooled porcine nasal swab and blood serum samples collected from both sides of the Dutch–German border region were evaluated. Overall, we detected 46 different viral species using TSC, compared to 40 viral species with a shotgun metagenomics approach. Furthermore, we performed phylogenetic analysis on recovered influenza A virus (FLUAV) genomes from Germany and revealed a close similarity to a zoonotic influenza strain previously detected in the Netherlands. Although TSC introduced coverage bias within the detected viruses, it improved sensitivity, genome sequence depth and contig length. In‐depth characterization of the swine virome, coupled with developing new enrichment techniques, can play a crucial role in the surveillance of circulating porcine viruses and emerging zoonotic pathogens.

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“…The PAstV isolated from wild boars has previously reported in several countries including Hungary [32], Slovakia [33], and Korea [20]. Especially, PAstVs isolated in Slovakia have been typed genetic variable lineages involved in PAstV-2, PAstV-4, and AstVs derived from different species (Chicken and bat) [33]. Whereas, Korean wild boar PAstV isolated in 2013 was limited in strain typed into PAstV-4 lineage [20].…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic analysis of kobuviruses and astroviruses from Korean wild boars: 2016–2018

et al. 2021

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The prevalence of porcine kobuvirus (PKoV) and porcine astrovirus (PAstV) in 845 Korean wild boars (KWB) during 2016-2018 were 28.0% and 10.6%, respectively, and co-infection of two viruses showed 5.1%. Phylogenetic tree analysis also revealed that 236 PKoVs from KWB were divided to diverse lineages within Aichivirus C group but the one strain (WKoV16CN-8627) was included the same cluster with bovine kobuvirus (Achivirus B). Eighty-nine PAstVs from KWB was belonged predominantly to lineage PAstV4 and only one strain (WAst17JN-10931) included novel to lineage PAstV2. Two viruses are epidemic more in young (≤ 12 months) than in old pigs (> 12 months).

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Genetic identification of astroviruses in wild boars

Cited by 5 publications

References 14 publications

Prevalence of Astroviruses in Different Animal Species in Poland

Prevalence of Astroviruses in Different Animal Species in Poland

Application of shotgun metagenomics sequencing and targeted sequence capture to detect circulating porcine viruses in the Dutch–German border region

Phylogenetic analysis of kobuviruses and astroviruses from Korean wild boars: 2016–2018

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