Genetic Identification of Closely Related Endangered Rhododendron Species from East Asia

Полежаева, М. А.; Iunusova, D. R.; Тихонова, Н. А.; Polezhaev, A. N.; Koldaeva, Marina N.

doi:10.1134/s1022795422010082

Cited by 2 publications

(2 citation statements)

References 13 publications

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“…(Polezhaeva et al 2018). Also it was revealed that cpDNA markers could be species-specific for two sympatric and hybridizing Rhododendron species -R. adamsii Rehder and R. parvifolium Adams (Polezhaeva et al 2022). But for the complex of Labrador teas in Sakhalin we obtained shared haplotypes and non-significant levels of genetic divergence between species.…”

Section: Discussionmentioning

confidence: 78%

Genetic diversity of the Rhododendron subsection Ledum species in Sakhalin Island: data from chloroplast DNA markers variability

Iunusova,

Polezhaeva,

Marchuk

2023

Bot Pac

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A high genetic variability was revealed in the populations of the Rhododendron subsection Ledum species complex in Sakhalin. In total, 94 individuals from natural populations of the four sympatric species of Labrador tea (R. tomentosum, R. subarcticum, R. tolmachevii and R. hypoleucum) were analyzed to evaluate the genetic variation in the territory of the Sakhalin Island and in two mainland populations. The haplotype diversity of five chloroplast intergenic spacers (trnH–psbA, petB–petD, 3’trnV (UAC)–ndhC, K2R–K707, atpB–rbcL) was quite high. There were 16 haplotypes (h1–h16) identified; h1, h2 and h4 are the most common, the rest were rare and no species-specific ones were found. Analysis of Molecular Variance (AMOVA) indicate that about 37 % of the genetic diversity is due to among population differences (FST = 0.37, p < 0.001). Molecular phylogenetic analysis based on chloroplast DNA data did not reveal a clear species structure, but revealed a trend towards the isolation of two genetic groups: including a group of samples from the southern and the middle parts of Sakhalin Island.

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Section: Discussionmentioning

confidence: 78%

Genetic diversity of the Rhododendron subsection Ledum species in Sakhalin Island: data from chloroplast DNA markers variability

Iunusova,

Polezhaeva,

Marchuk

2023

Bot Pac

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“…This indicates the need to introduce more wild genotypes into breeding programs of daylilies to extend the gene pool. A similar problem is seen in other plants: Nebulo [36], Oryza [37], Lycoris [38], Rhododendron [39], and Narcissus [40] species to overcome the bottleneck of low genetic diversity and gene flow. Increasing genetic variation in daylily breeding programs by adding modern cultivars and early hybrids and species could ensure the diversity of new cultivars.…”

Section: Population Structure Genetic Diversity and Relationships Of ...mentioning

confidence: 74%

Assessing the Genetic Diversity of Daylily Germplasm Using SSR Markers: Implications for Daylily Breeding

Misiukevičius

Frercks

Šikšnianienė

et al. 2023

Plants

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This work aims to characterize the genetic diversity of species, early hybrids, and cultivars using microsatellite simple sequence repeat (SSR) markers, as well as analyze and identify the origin of Hemerocallis spp. early hybrids. For this research, samples were collected from different types of daylily species, early hybrids (known or hypothetically first-generation hybrids from Hemerocallis species), foreign, and Lithuanian varieties. An initial screening of SSR primers developed for Hemerocallis citrina was performed, and their suitability for testing other daylily species and hybrids was evaluated. The genetic diversity was assessed with the selected eight-primer set, and molecular SSR profiles were created. Primer SAU00097 is the most informative according to heterozygosity (0.95) and polymorphism information content (PIC) (0.17). The highest heterozygosity was observed in Lithuanian cultivars (0.713), the lowest in species (0.583). Genetic relationships between species show that only fulvous daylilies are separated into a different cluster. The highest variation among genotypes was observed in the species group (18%), while modern cultivars had the slightest variation among genotypes (1%). The putative origin of early hybrids was analyzed using a likelihood heatmap of all genotypes. Results show what species might be used in breeding for early hybrids. Several modern diploid and tetraploid daylily cultivars have triploid species as ancestors.

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Genetic Identification of Closely Related Endangered Rhododendron Species from East Asia

Cited by 2 publications

References 13 publications

Genetic diversity of the Rhododendron subsection Ledum species in Sakhalin Island: data from chloroplast DNA markers variability

Genetic diversity of the Rhododendron subsection Ledum species in Sakhalin Island: data from chloroplast DNA markers variability

Assessing the Genetic Diversity of Daylily Germplasm Using SSR Markers: Implications for Daylily Breeding

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