Genetic identification of three species of the genus Clarias using allozyme and mitochondrial DNA markers

Mohindra, Vindhya; Singh, Rajeev K.; Palanichamy, Malliya Gounder; Ponniah, A. G.; Lal, Kuldeep K.

doi:10.1111/j.1439-0426.2006.00795.x

Cited by 17 publications

(12 citation statements)

References 25 publications

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“…Among Siluriformes, the application of such methodology has proven to be useful to distinguish species and their hybrid forms and to identify potential markers for conservation and management programs. These studies involved species of the genus Clarias (Mohindra et al, 2007), whose distinct species were easily discriminated, and each population presented some specific haplotypes that were helpful in their identification.…”

Section: Molecular Datamentioning

confidence: 99%

Comparative application of direct sequencing, PCR-RFLP, and cytogenetic markers in the genetic characterization of Pimelodus (Siluriformes: Pimelodidae) species: Possible implications for fish conservation

et al. 2014

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ABSTRACT. Pimelodus (Pimelodidae) is a genus comprising a group of South American species with complex taxonomic relationships. Cytogenetics, polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP), and sequencing data of mitochondrial genes were analyzed to characterize 4 Pimelodus species: P. fur, P. heraldoi, P. maculatus, and Pimelodus sp. All populations presented 2n = 56 chromosomes and distinct karyotypic formulae. The heterochromatin distribution pattern and the number and location of 5S and 18S rDNA sites are discussed. The application of PCR-RFLP markers and sequencing of mitochondrial DNA genes provided species-specific haplotypes, which allowed us to differentiate the species studied. The mitochondrial gene sequences presented nucleotide mutations in the restriction sites and throughout the sequences, and they were mostly related to synonymous substitutions in the coded proteins; however, they did not affect the protein and its function. Comparing the data obtained using these 3 methodologies, the existence of a species complex in P. maculatus along the basins studied might be inferred, showing that cytogenetics is an important tool in studies focusing on the conservation or management of both natural and captive populations of these fishes.

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Section: Molecular Datamentioning

confidence: 99%

Comparative application of direct sequencing, PCR-RFLP, and cytogenetic markers in the genetic characterization of Pimelodus (Siluriformes: Pimelodidae) species: Possible implications for fish conservation

et al. 2014

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“…The reports are scanty on barcoding of clariids found in India. Genetic variability in three clariid species, C. batrachus, C. gariepinus, and C. macrocephalus, were investigated using allozyme electrophoresis and mitochondrial DNA (ND 5/6) RFLP markers by Mohindra et al (2007). Mitochondrial DNA has been extensively studied in fish phylogenetics since mitochondrial 16S rRNA gene and the protein coding cytochrome C Oxidase subunit I (COI) genes are highly conserved (Lakra et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Genetic identification and phylogenetic relationships of Indian clariids based on mitochondrial COI sequences

Devassy

Kumar

Shajitha

et al. 2015

Mitochondrial DNA Part A

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Mitochondrial cytochrome C Oxidase I (COI) sequence variation among the clariid fishes of India (Clarias magur, C. dussumieri and C. gariepinus) and their relationship with other representative clariids was studied in this work. Three species were sampled and together with 23 COI sequences from GenBank were used to reconstruct phylogenetic relationships in the family Clariidae. The study revealed two clades: one consisting of the African species with C. dussumieri, and the other of Asian species suggesting the prevalence of intra-continental diversification of catfishes. This study further revealed that the genus Clarias is monophyletic. For the COI gene, the interspecies genetic divergence ranged from 0.056 to 0.182. The mean genetic difference between C. dussumieri and other selected African species in this study is 12.1%. It was also observed that the morphological similarity of C. dussumieri and C. magur was not replicated in the genetic level. Clarias dussumieri was more close to African catfish C. gariepinus thus indicating the utility of COI phylogeny to identify the well-known African-Asian relationships within catfishes. The results also showed that C. magur and C. batrachus are genetically distinct from each other.

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“…and reported a high numerical and positional variation of 5S rDNA sites, variation not observed for others heptapterids genus as Rhamdia . DNA molecular markers have been successfully applied on natural fish populations to establish genetic and evolutionary relationships within groups, besides assisting species conservation (Triantafylli, 1999;Mohindra, 2007). Amongst the molecular techniques related to mitochondrial DNA is quite useful since it provides information on levels of intra and interspecific polymorphisms, thereby allowing defining evolutionary relationships among studied groups (Moysés & Almeida-Toledo, 2002).…”

Section: Introductionmentioning

confidence: 99%

Cytogenetic and molecular analyses in troglobitic and epigean species of Pimelodella (Siluriformes: Heptapteridae) from Brazil

et al. 2012

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Samples from seven different locations of the genus Pimelodella were genetically examined, two caves (exclusively subterranean, upper Tocantins River and São Francisco River) and five epigean (from upper Paraná River basin). Cytogenetic analyses revealed the same diploid number (2n=46) for all species besides similarities in both number and location of nucleolar organizer regions and C bands. FISH with 5S rDNA probes and CMA 3 staining indicated significant differences among the studied species. Application of PCR-RFLP in ATPase 6 and 8 mitochondrial genes allowed building a minimum evolution phenogram identifying the close evolutionary relationship among groups. Both chromosomal and molecular data were useful to infer the relationships among studied Pimelodella species.Amostras de sete diferentes localidades do gênero Pimelodella foram geneticamente analisadas, duas cavernícolas (exclusivamente subterrâneas, alto rio Tocantins e rio São Francisco) e cinco epígeas (provenientes da bacia do alto Paraná). Análises citogenéticas revelaram o mesmo número diploide (2n=46) para todas as espécies, além de similaridades no número e localização das regiões organizadoras de nucléolo e bandas C. FISH com sondas de rDNA 5S e marcação com CMA 3 indicaram diferenças significativas entre as espécies estudadas. A aplicação da técnica de PCR-RFLP nos genes mitocondriais ATPase 6 e 8 permitiu a construção de um fenograma de evolução mínima identificando uma estreita relação evolutiva entre as espécies estudadas.

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Genetic identification of three species of the genus Clarias using allozyme and mitochondrial DNA markers

Cited by 17 publications

References 25 publications

Comparative application of direct sequencing, PCR-RFLP, and cytogenetic markers in the genetic characterization of Pimelodus (Siluriformes: Pimelodidae) species: Possible implications for fish conservation

Comparative application of direct sequencing, PCR-RFLP, and cytogenetic markers in the genetic characterization of Pimelodus (Siluriformes: Pimelodidae) species: Possible implications for fish conservation

Genetic identification and phylogenetic relationships of Indian clariids based on mitochondrial COI sequences

Cytogenetic and molecular analyses in troglobitic and epigean species of Pimelodella (Siluriformes: Heptapteridae) from Brazil

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